Probiyotik Yoğurda Böğürtlen ve Yulaf Kepeği İlavesinin Lactobacillus acidophilus Canlılığı ve Antioksidan Aktivite Üzerine Etkisi

Bu çalışmada yulaf kepeği ve böğürtlen ayrı ayrı ve birlikte probiyotik yoğurt (Lactobacillus acidophilus) üretiminde kullanılmıştır. Yoğurtların hem soğukta depolanma süreleri hem de in vitro gastrointestinal sindirim aşamalarında probiyotik canlılığı, toplam fenolik madde miktarı ve antioksidan aktivite düzeyleri (DPPH, ABTS, CUPRAC yöntemleri) üzerine yulaf kepeği ve/veya böğürtlenin etkisinin tespiti amaçlanmıştır. 21 günlük depolama süresi sonunda en yüksek ve en düşük L. acidophilus canlılığının sırasıyla böğürtlen içeren (B) (107 kob/g) ve yulaf kepeği içeren (Y) yoğurdun (105 kob/g) sahip olduğu belirlenmiştir. Depolama süresinin başlangıcında ve sonunda en yüksek ve en düşük toplam fenolik madde düzeyine sırasıyla yulaf kepeği ve böğürtlen içeren YB yoğurdu ile kontrol yoğurdu sahip olmuştur. DPPH yöntemiyle saptanan antioksidan aktivite sonuçlarına göre B yoğurdu, ABTS ve CUPRAC yöntemine göre YB yoğurdu en yüksek antioksidan aktiviteye sahip olmuştur. Sonuç olarak yulaf kepeği ve böğürtlen ilavesiyle probiyotik yoğurdun fonksiyonel özelliklerinin daha da geliştirilebileceği ve böğürtlenin L. acidophilus üzerinde prebiyotik etki gösterebileceği ortaya konmuştur.

Effect of Blackberry and Oat Bran Addition on Lactobacillus acidophilus Viability and Antioxidant Activity of Probiotic Yogurt

In this study, oat bran and blackberry were used separately and together in the production of probiotic yogurt (Lactobacillus acidophilus). It was aimed to determine the effect of oat bran and/or blackberry on probiotic viability, total phenolic content and antioxidant activity levels (DPPH, ABTS, CUPRAC methods) of yoghurts during both cold storage times and in vitro gastrointestinal digestion stages. At the end of the 21 day of storage period, it was determined that the highest and lowest L. acidophilus viability was found in yoghurt containing blackberry (B) (107 cfu/g) and oat bran (Y) (105 cfu/g), respectively. Oat bran and blackberry containing yogurt YB and control yogurt had the highest and lowest total phenolic content at the beginning and end of the storage period, respectively. According to the antioxidant activity results determined by the DPPH method, B yogurt had the highest antioxidant activity, while according to the ABTS and CUPRAC methods, YB yogurt had the highest antioxidant activity. As a result, it has been revealed that the functional properties of probiotic yogurt can be further improved with the addition of oat bran and blackberry, and blackberry can have a prebiotic effect on L. acidophilus.

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Akan E. 2021. An evaluation of the in vitro antioxidant ve antidiabetic potentials of camel and donkey milk peptides released from casein and whey proteins. Journal of Food Science and Technology, 58(10): 3743-3751. doi: 10.1007/s13197-020-04832-5.
Alberto WD, Marıa del Pilar Da, Marıa Valeria A, Fabiana PS, Cecilia HA, Marıa De Los Ángeles B. 2001. Pattern recognition techniques for the evaluation of spatial ve temporal variations in water quality. A case study: Suquıa river basin (Córdoba–Argentina). Water Research, 35: 2881- 2894, doi: 10.1016/S0043-1354(00)00592-3
Angelov A, Gotcheva V, Kuncheva R, Hristozova T. 2006. Development of a new oat based probiotic drink. International Journal of Food Microbiology, 112(1): 75-80.
Anuyahong T, Chusak C, Adisakwattana S. 2020. Incorporation of anthocyanin-rich riceberry rice in yogurts: Effect on physicochemical properties, antioxidant activity ve in vitro gastrointestinal digestion. LWT-Food Science and Technology, 129: 109571.
AOAC, 2003. Official methods of analysis’’, (17th edition). Washington, DC, USA: Association of Analytical. Bakkalbasi E, Mentes Ö, Artik N. 2009. Food ellagitannins- occurrence, effects of processing ve storage. Critical Reviews in Food Science and Nutrition, 49: 283-298.
Barat A, ozcan T. 2018. Growth of probiotic bacteria and characteristics of fermentes milk containing fruit matrices, International Journal of Dairy Technology, 71(S1): 120-129.
Chait YA, Gunenc A, Bendali F, Hosseinian F. 2021. Functional fermented carob milk: Probiotic viability and polyphenolic profile. Journal of Food Bioactives, 14: 114–125.
Chen C, Wang L, Wang R, Luo X, Li Y, Li J. 2018.Phenolic contents, cellular antioxidant activity and antiproliferative capacity of different varieties of oats. Food Chemistry, 239: 260-267.
Coman I, Pola-Bibián B, Barranco P, Vila-Nadal G, Dominguez- Ortega J, Romero D, Villasante C, Quirce S. 2018.
Bronchiectasis in severe asthma: Clinical features ve outcomes. Annals of Allergy, Asthma and Immunology,120(4): 409-413. doi: 10.1016/j.anai.2018.02.0 16.
Darwish AA. 2021. Using in vitro digestion method in assesing the vialability of Lactobacillus spp. in White soft cheese-like products. Journal of Food and Dairy Sciences, 12(3): 65-71.
De Carvalho MW, Arriola NA, Pinto S, Verruck S, Fritzen-Freire CB, Prudêncio ES, Amboni RD. 2019. Stevia-fortified yoghurt: Stability, antioxidant activity ve in vitro digestion behaviour. International Journal of Dairy Technology, 72: 57-64.
Demirci T, Aktaş K, Sözeri D, Öztürk H, Akın N. 2017. Rice bran improve probiotic viability in yoghurt ve provide added antioxidative benefits. Journal of Functional Foods, 36: 396-403.
Dimitrellou D, Solomakou N, Kokkinomagoulos E, Kandylis P. 2020. Yogurts supplemented with juices from grapes ve berries. Foods, 9(9): 1158.
Espírito Santo AP, Cartolano NS, Silva TF, Soares FA, Gioielli LA, Perego P, Converti A, Oliveira MN. 2011. Fibers from fruit by-products enhance probiotic viability ve fatty acid profile ve increase CLA content in yoghurts. International Journal of Food Microbioly, 154(3):135-44. doi: 10.1016/j.ijfoodmicro.2011.12.025.
Gibson G, Hutkins R, Sanders M, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, Verbeke K, Reid G. 2017. Expert consensus document: The International Scientific Association for Probiotics ve Prebiotics (ISAPP) consensus statement on the definition ve scope of prebiotics. Nature Reviews Gastroenterology and Hepatology, 14: 491–502. doi: https://doi.org/10.1038/nrgastro.2017.75
Güler-Akın M, Ferliarslan I, Akın M. 2016. Apricot Probiotic Drinking Yoghurt Supplied with Inulin ve Oat Fiber. Advances in Microbiology, 6: 999-1009.
Helal, A, Tagliazucchi D. 2018. Impact of in-vitro gastro- pancreatic digestion on polyphenols ve cinnamaldehyde bioaccessibility ve antioxidant activity in stirred cinnamon- fortified yogurt. LWT-Food Science and Technology, 89: 164-170.
Hasani S, Khodadadi I, Heshmati A. 2016. Viability of Lactobacillus acidophilus in rice bran-ennriched stirred yoghurt ve the physicochemical ve sensory characteristics of product during refrigerated storage. International Journal of Food Science and Technology, 51: 2485–2492.
Hasani S, Sari AA, Heshmati A, Karami M. 2017. Physicochemical ve sensory attributes assessment of functional low-fat yogurt produced by incorporation of barley bran ve Lactobacillus acidophilus. Food Science and Nutrition, 5(4): 875-880.
Heshmati A, Hasani S, Sari A, Karami M. 2016. Effect of Prebiotics Oat ve Rice. Food Technology, 11(2): 105- 112.
Heshmati A, Hasani S, Sari AA, Karami M. 2015. Effect of Prebiotics Oat ve Rice Bran on Lactobacillus acidophilus in Low-fat Yogurt. Iranian Journal of Nutrition Sciences and Food Technology, 11(2): 105-112.
Hitayezu R, Baakdah MM, Kinnin, J, Henderson K, Tsopmo A. 2015. Antioxidant activity, avenanthramide and phenolic acid contents of oat milling fractions. Journal of Cereal Science, 63: 35-40.
Jastrebova J, Skoglund M, Nilson J, Dimberg LH. 2006. Selective and sensitive LC-MS determination of avenanthramides in oats. Chromatographia, 63: 419-423
Kamber U, Harmankaya S. 2019. The effect of fruits to the characteristics of fruit yoghurt. Pakistan Journal of Agricultural Research, 56(2): 495-502.
Kilic GB, Akpinar D. 2013. The effects of different levels of β- glucan on yogurt manufactured with Lactobacillus plantarum strains as adjunct culture. Journal of Food, Agriculture and Environment, 11(1): 281-287.
Kilic GB, Kankaya DA. 2016. Assessment of technological characteristics of non-fat yogurt manufactured with prebiotics ve probiotic strains. Journal of Food Science and Technology, 53(1): 864-871.
Lamothe M, Boujut E, Zenasni F, Sultan S. 2014. To be or not to be empathic: the combined role of empathic concern ve perspective taking in understanding burnout in general practice. BMC Famiy Practice, 15: 15. doi: https://doi.org/10.1186/1471-2296-15-15
Landete JM. 2011. Ellagitannins, ellagic acid ve their derived metabolites: A review about source, metabolism, functions ve health. Food Research International. 44 (5): 1150-1160
Landete JM. 2012. Updated Knowledge about Polyphenols: Functions, Bioavailability, Metabolism, ve Health. Critical Reviews in Food Science ve Nutrition, 52: 936-948.
Lazaridou A, Serafeimidou A, Biliaderis C, Moschakis T, Tzanetakis N. 2014. Structure development ve acidification kinetics in fermented milk containing oat b-glucan, a yogurt culture ve a probiotic strain. Food Hydrocolloids, 39: 204- 214.
Lee J, Dossett M, Finn CE. 2012. Rubus fruit phenolic research: The good, the bad, and the confusing. Food Chemistry, 130: 785-796.
Lee MJ, Kim K, Kim Y, Park J, Kim H, Choi J, Kim K. 2013. Quality characteristics ve antioxidant activity of yogurt added with whole barley flour. Korean Journal of Food Science and Technology, 45: 6.
Liu RH. 2013. Health-promoting components of fruits ve vegetables in the diet. Advances in Nutrition, 4(3): 384-392.
Mabrouk R, Berrezouga L, Frih N. 2021. The Accuracy of CBCT in the Detection of Dens Invaginatus in a Tunisian Population. International Journal of Dentistry, 8826204.
Minekus M, Alminger M, Alvito P, Ballance S, Bohn T, Bourlieu C, Carrière F, Boutrou R, Corredig M, Dupont D, Dufour C, Egger L, Golding M, Karakaya S, Kirkhus B, Le Feunteun, S, Lesmes U, Macierzanka A, Mackie A, Marze S, McClements DJ, Ménard O, Recio I, Santos CN, Singh RP, Vegarud GE, Wickham MS, Weitschies W, Brodkorb A. 2014. A standardised static in vitro digestion method suitable for food–an international consensus. Food and Function, 5(6): 1113-1124.
Petrova P, Petrov KK. 2020. Lactic acid fermentation of cereals and pseudocereals: ancient nutritional biotechnologies with modern applications. Nutrients, 12(4): 1118. https://doi.org/10.3390/nu12041118
Pojer E, Mattivi F, Johnson D, Stockley CS. 2013. The Case for Anthocyanin Consumption to Promote Human Health: A Review. Comprehensive Reviews in Food Science ve Food Safety, 12(5): 483- 508.
Rodríguez-Costa S, Cardelle-Cobas A, Roca-Saavedra P, Porto- Arias JJ, Miranda JM, Cepeda A. 2018. In vitro evaluation of the prebiotic effect of red ve white grape polyphenolic extracts. Journal of Physiology ve Biochemistry, 74(1): 101–110.
Rosburg V, Boylston T, White P. 2010. Viability of Bifidobacteria Strains in Yogurt with Added Oat Beta-Glucan ve Corn Starch during Cold Storage. Journal of Food Science, 75(5): 439-444.
Ryhänen EL, Mantere-Alhonen S, Salovaara H. 1996. Effects of oat bran ve rye bran diet on intestinal Lactobacillus ve Bifidobacterium flora on Wistar rats: Dietary Fiber ve Fermentation in the Colon. 55-57 p. Ed: Y. Mälkki JH.
Cummings. Luxembourg: Office for Official Publications of European Communities Saccaro DM, Hirota CY, Tamime AY, Oliveira MGD. 2011. Evaluation of different selective media for enumeration of probiotic micro-organisms in combination with yogurt starter cultures in fermented milk. African Journal of Microbiology Research, 5(23): 3901–3906.
Savaş BS, Akan E. 2021. Oat bran fortified raspberry probiotic dairy drinks: Physicochemical, textural, microbiologic properties, in vitro bioaccessibility of antioxidants ve polyphenols. Food Bioscience, 43:101223.
Senaka Ranadheera C, Evans CA, Adams MC, Baines SK. 2012. Probiotic viability ve physico-chemical ve sensory properties of plain ve stirred fruit yogurts made from goat’s milk. Food Chemistry, 135(3): 1411–1418.
Terzaghi BE, Sandine W. 1975. Improved medium for lactic streptococci ve their bacteriophages. Applied Microbiology, 29(6): 807-813.
Yousef M, Mateghi L, Azadi E. 2013. Effect of different concentration of food additives on some physicochemical properties of yoghurt during storage. Annals of Biological Research, 4: 244-249.
Tharmaraj N, Shah NP. 2003. Selective Enumeration of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus ve Propionibacteria. Journal of Dairy Science, 86: 2288–2296.
Tomas M, Rocchetti G, Ghisoni S, Giuberti G, Capanoglu E, Lucini L. 2020. Effect of different soluble dietary fibres on the phenolic profile of blackberry puree subjected to in vitro gastrointestinal digestion ve large intestine fermentation. Food Research International, 130: 108954.