Ayçiçek Küspesinden Elde Edilen Protein Hidrolizatlarının Anjiyotensin Dönüştürücü Enzim ve Dipeptidil Peptidaz–IV İnhibisyon Aktiviteleri

Bu çalışmada ayçiçek protein hidrolizatlarının anjiyotensin dönüştürücü enzim (ADE) ve dipeptidil peptidaz-IV (DPP-IV) enzimini inhibe edebilme potansiyeli saptanmıştır. Ayçiçek protein izolatı Alkalaz ve Tripsin+Kimotripsin enzimleri ile hidrolizlenmiş ve elde edilen protein hidrolizatları ultrafiltrasyon ile fraksiyonlanmıştır (5 kDa). Alkalaz ve Tripsin+Kimotripsin enzimleri ile ulaşılan hidroliz dereceleri sırasıyla %27 ve %13 olmuştur. En yüksek ADE inhibisyon aktivitesi (IC50=0,06 ± 0,01 mg/mL) Tripsin+Kimotripsin enzimi ile hidrolizlenen ve molekül ağırlığı 5 kDa’dan düşük olan fraksiyonda gözlenmiştir (P

Angiotensin Converting Enzyme and Dipeptidyl Peptidase-IV Inhibitory Activity of Protein Hydrolysates Obtained from Sunflower Meal

In this study, the potential of sunflower protein hydrolysates to inhibit angiotensin converting enzyme (ACE) and dipeptidyl peptidase-IV (DPP-IV) enzyme was determined. Sunflower protein isolate was hydrolyzed with Alcalase and Trypsin+Chymotrypsin enzymes and the obtained protein hydrolysates were fractionated by ultrafiltration (5 kDa). The degrees of hydrolysis achieved by Alcalase and Trypsin+Chymotrypsin enzymes were 27% and 13%, respectively. The most potent ACE inhibitory activity (IC50=0.06 ± 0.01 mg/mL) was observed for the fraction that has molecular weight below 5 kDa, which was hydrolyzed by Trypsin+Chymotrypsin enzyme (P

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