MAHMUT ÇINAR, Aydın GÜZELOĞLU, HÜSEYİN ERDEM

Effect of presence of corpus luteum at the beginning of Ovsynch protocol on pregnancy rates in lactating dairy cows

Bu çalışmada, laktasyondaki süt ineklerinde Ovsynch protokolüne başlarken CL varlığının gebelik oranları üzerine etkisinin araştırılması amaçlandı. Çalışmada en az 45 gün önce doğum yapmış ve reprodüktif sorunu bulunmayan 218 baş Holstein ırkı inek kullanıldı. İnekler, transrektal ultrasonografik muayenin ilk gününde (0. gün) korpus luteum (CL)’un varlığı ve yokluğuna göre iki gruba ayrıldı. CL’a sahip olan inekler (CL (+)) rastgele iki alt gruba ayrıldı. CL(+)/A grubundaki (n = 74) ineklere CL belirlenmesinin hemen ardından Ovsynch protokolüne başlanılırken, CL(+)/B grubundaki (n = 78) ineklere CL belirlenmesinin ardından PGF2α enjeksiyonu uygulanarak 7 gün sonra Ovsynch protokolüne başlanıldı. CL (-) grubundaki (n = 66) ineklere ise transrektal muayene gününden sonraki 7. günde Ovsynch protokolü başlatıldı. Gebelik oranları CL(+)/A, CL(+)/B ve CL (-) gruplarında sırasıyla %23.0, %25.6, %40.9 olarak elde edildi. CL (-) grubundaki gebelik oranının CL(+)/A ve CL(+)/B gruplarına göre önemli derecede yüksek olduğu belirlendi (P

Anahtar Kelimeler:

inek, sığır, sarı cisim, süt sığırı, prostaglandinler, laktasyon, kızgınlık, yumurtalıklar, gebelik, gebelik oranları, dölverim performansı, üreme özellikleri, eş zamanlama

Laktasyondaki süt ineklerinde Ovsynch protokolüne başlarken korpus luteum varlığının gebelik oranları üzerine etkisi

The aim of the study was to investigate the effect of presence of CL at the beginning of Ovsynch protocol on pregnancy rates in lactating dairy cows. A total of 218 lactating Holstein cows that were at least 45 days postpartum with no reproductive disorders were used in the present study. Cows were divided into two experimental groups according to the presence and absence of corpus luteum (CL) on the first day of transrectal ultrasonography (day 0). Cows having CL (CL (+)) were randomly allocated to two subgroups as CL (+)/A and CL (+)/B. Cows in CL (+)/A group (n = 74) were treated with Ovsynch protocol, while cows in CL (+)/B group (n = 78) were treated with PGF2a on the first day of examination and seven days later Ovsynch protocol was started. Cows having no CL (CL (-)) (n = 66) were left untreated on the first day of examination and Ovsynch protocol was started seven days later. Pregnancy rates were found 23%, 25.6% and 40.9% in CL (+)/A, CL (+)/B and CL (-) groups, respectively. The pregnancy rate in CL (-) group was significantly higher than those of CL (+)/A, and CL (+)/B groups (P<0.05). It is suggested that the success of Ovsynch synchronization protocol may be related to starting time of the protocol and to ovarian physiology in cyclic dairy cows.

Keywords:

cows, cattle, corpus luteum, dairy cattle, prostaglandins, lactation, oestrus, ovaries, pregnancy, pregnancy rate, reproductive performance, reproductive traits, synchronization,

PDF

___

1. Mateus L, Costa LL, Cardos JJA, Silva JR: Treatment of unobserved oestrus in a dairy cattle herd with low oestrous detection rate up to 60 days postpartum. Reprod Dom Anim, 37, 1, 57-60, 2002.
2. Yalçın C: Süt sığırcılığında infertiliteden kaynaklanan kayıplar. Lalahan Hay Araş Enst Derg, 40 (1): 39-47, 2000.
3. Robert MD: Ovulation synchronization and fixed time artificial insemination in dairy cows: Problems encountered with poor follicle welfare. http://ag.udel.edu/anfs/faculty/kung/documents/Summer08 AugDairyNewsletter.pdf. Accessed: 10 June 2011.
4. Jordan ER: Understanding Ovsynch. http://texasdairymatters.org/files/2010/06/, http://texasdairymatters.org/files/2010/06/Understanding -Ovsynch.pdf, Accessed: 10.09.2011.
5. Pursley JR, Mee MO, Wiltbank MC: Synchronization of ovulation in dairy cows using PF2α and GnRH. Theriogenology, 52, 1067-1078, 1995.
6. Moreira F, de la Sota RL, Diaz T, Thatcher WW: Effect of day of the estrous cycle at the initiation of a timed artificial insemination protocol on reproductive responses in dairy heifers. J Anim Sci, 78, 1568-1576, 2000.
7. Vasconcelos JLM, Silcox RW, Rosa GJM, Pursley JR, Wiltbank MC: Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. Theriogenology, 52 (6): 1067-1078, 1999.
8. EL-Zarkouny SZ, Cartmill JA, Hensley BA, Stevenson JS: Pregnancy in dairy cows after synchronized ovulation regimens with or without presynchronization and progesterone. J Dairy Sci, 87, 1024-1037, 2004.
9. Moreira F, Orlandi C, Risco CA, Mattos R, Lopes L, Thatcher WW: Effects of presynchronization and bovine somatotropin on pregnancy rates to a timed AI protocol in lactating dairy cows. J Dairy Sci, 84, 1646-1659, 2001.
10. Cartmill JA, El-Zarkouny SZ, Hensley BA, Lamb GC, Stevenson JS: Stage of cycle, incidence, and timing of ovulation, and pregnancy rates in dairy cattle after three timed breeding protocols. J Dairy Sci, 84, 1051-1059, 2001.
11. Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Garverick HA, Anderson LL: Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci, 80, 295-300, 1997.
12. Jemmeson A: Synchronising ovulation in dairy cows with either two treatments of gonadotropin-releasing hormone and one of prostaglandin, or two treatments of prostaglandin. Aust Vet J, 78, 108-111, 2000.
13. Stevenson JS, Kobayashi Y, Thompson KE: Reproductive performance of dairy cows in various programmed breeding systems including Ovsynch and combinations of gonadotropinreleasing hormone and prostaglandin F2α. J Dairy Sci, 82, 506-515, 1999.
14. Silva E, Sterry RA, Kolb D, Fricke PM: Assessment of a practical method for identifying anovular dairy cows synchronized for first postpartum timed artificial insemination. J Dairy Sci, 90, 3255-3262, 2007.
15. Tenhagen BA, Kuchenbuch S, Heuwieser W: Timing of ovulation and fertility of heifers after synchronization of oestrus with GnRH and prostaglandin F2α. Reprod Dom Anim, 40, 62-67, 2005.
16. Rivera H, Sterry RA, Fricke PM: Presynchronization with gonado-tropin-releasing hormone does not improve fertility in Holstein heifers. J Dairy Sci, 89, 3810-3816, 2006.
17. Yılmaz C, Yılmaz O, Uçar M: Effect of PGF2α and GnRH injections applied before Ovsynch on pregnancy rates in cows and heifers. Kafkas Univ Vet Fak Derg, 17 (4): 641-644, 2011.
18. Nak Y, Tuna B, Nak D, Karataş E, Şimşek G: The effects of Ovsynch, Ovsynch with progestin and progestin plus double TAI on pregnancy rates in unobserved oestrus dairy cows and heifers. Kafkas Univ Vet Fak Derg, 17 (6): 917-922, 2011.
19. National Research Councıl: Nutrient Requirements of Dairy Cattle. 7th ed., National Academy Press, Washington, D C, 2001.
20. Mialot JP, Laumonnier G, Fauxpoint H, Barassin E, Ponter AA, Deletang F: Postpartum subestrus in dairy cows: Comparison of treatment with prostaglandin F2 alpha or GnRH + prostaglandin F2 alpha + GnRH. Theriogenology, 52, 901-911, 1999.
21. Amiridis GS, Belibasaki S, Leontides L, Lymberopoulos A, Vainas E: Reproductive efficiency of three estrus ynchronization schemes comprising fixed time insemination in dairy cows. J Vet Med A, 47, 271-276, 2000.
22. Sartori R: Fertilization and embryonic mortality in cattle. Acta Sci Vet, 32, 35-50, 2004.
23. Lean IJ, Porter JA, Rabiee AR, Morgan WF, Tranter WP, Moss N, Rheinberger RJ: Comparison of effects of GnRH and prostaglandin in combination, and prostaglandin on conception rates and time to conception in dairy cows. Aust Vet J, 81 (8): 488-493, 2003.
24. Lopez-Gaitus F, Murugavel K, Santolaria P, Lopez-Bejar M, Yanız JL: Pregnancy rate after timed artificial insemination in early post-partum dairy cows after Ovsynch or specific synchronization protocols. J Vet Med A Physiol Pathol Clin Med, 51 (1): 33-38, 2004.
25. Santos JEP, Thatcher WW, Chebel RC, Cerri RLA, Galvao KN: The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Anim Reprod Sci, 82-83, 513-535, 2004.
26. Tenhagen BA, Surholt R, Wittke M, Vogel C, Drillich M, Heuwieser W: Use of Ovsynch in dairy herds-differences between primiparous and multiparous cows. Anim Reprod Sci, 81, 1-11, 2004.
27. Kırbaş M, Çoyan K, Bülbül B, Ataman MB, Köse M, Akman O, Dursun Ş: İnek ve düvelerde luteal aktivitenin Ovsynch protokolüne etkisi. Uludağ Univ J Fac Vet Med, 27, 47-52, 2008.
28. Cirit U, Ak K, Ileri K : New strategies to improve the efficiency of the Ovsynch protocol in primiparous dairy cows. Bull Vet Inst Pulawy, 51, 47-51, 2007.
29. Moreira F, DeLaSota RL, Diaz T and Thatcher WW: Effect of day of estrous cycle at the initiation of a timed artificial insemination protocol on reproductive responses in dairy heifers. J Anim Sci, 78, 1568-1576, 1999.
30. Souza AH, Ayres H, Ferreira RM, Wiltbank MC: A new pre-synchronization system (Double-Ovsynch) increases fertility post-partum timed AI in lactating dairy cows. Theriogenology, 70, 208-215, 2008.
31. Maiero S, Renaville B, Comin A, Marchini E, Fazzini U, Prandi A, Motta M, Marchi V: Effect of cyclicity on Ovsynch synchronization treatment efficiency in dairy cows. J Anim Vet Adv, 5 (12): 1062-1066, 2006.