Antibacterial effects of ethanol and acetone extract of Plantago major L. on gram positive and gram negative bacteria

Çalışmamızda, Plantago major L. yapraklarından elde edilen aseton ve etil alkol ekstraktlarının Makrodilusyon sıvı (tüp) yöntemi ile Minimum inhibitör konsantrasyonları (MİK) tespit edilmiştir. Her iki ekstrakt dokuz farklı bakteri türü (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella enteritidis) üzerinde test edilmiştir. Etil alkol ekstraktının test bakterilerinden E. coli ve B. cereus dışındaki diğer bakterilere karşı antibakteriyel etkinlik göstermediği saptanırken, aseton ekstraktının ise kullanılan bakteri suşlarına karşı farklı konsantrasyonlarda etkinlik gösterdiği görülmüştür

Anahtar Kelimeler:

Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Plantago major, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, antibakteriyel özellikler, Staphylococcus epidermidis, gram negatif bakteriler, gram pozitif bakteriler, in vitro, yapraklar, tıbbi bitkiler, patojenler, farmakoloji, bitki özütü

Gram pozitif ve gram negatif bakteriler üzerine Plantago major L.’nin etanol ve aseton ekstraktlarının antibakteriyel etkisi

In this study, minimum inhibitor concentrations of acetone and ethyl alcohol extracts of Plantago major L. leaves on predetermined bacteria species was determinbed by Macrodilution liquid (tube) method. Both extracts were tested for nine bacteria species (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella enteritidis). Ethyl alcohol extract showed no antibacterial activity against all species (except for E. coli and B. cereus), but acetone extract was effective on selected bacteria species in different concentrations.

Keywords:

Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Plantago major, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, antibacterial properties, Staphylococcus epidermidis, Gram negative bacteria, Gram positive bacteria, in vitro, leaves, medicinal plants, pathogens, pharmacology, plant extracts,

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