ŞAHVER EGE HİŞMİOĞULLARI, ÖZLEM YAVUZ, Cemal KARA, ADNAN ADİL HİŞMİOĞULLARI, Kamil SEYREK, ARMAĞAN HAYIRLI, İsmail YAMAN, ÖMÜR KARACA SAYGILI

The effect of amniotic membrane on serum biochemical parameters in experimentally ınduced non-sterile clean wound inflammation

Deneysel olarak indüklenmiş steril olmayan temiz yara inflammasyonuna cevap olarak, amniyotik membranın, biyokimyasal parametreler üzerine etkisi incelendi. Ayrıca insan plasenta ekstraktının biyokimyasal bileşenleri de araştırıldı. Ağırlıkları 250-300 g arasında değişen, 30 adet, erkek Wistar-Albino sıçanları kullanıldı ve rastgele 10’arlı 3 grup oluşturuldu. Kontrol grubu hiçbir operasyon geçirmezken, sham-operasyonlu grup, deneysel temiz yara inflammasyonuna maruz kaldı ve amniyotik membran grubu da deneysel temiz yara inflammasyonuna maruz kalıp HAM sağaltımı aldı. Sıçanların hepsi, standart laboratuvar diyeti ve suya serbest ulaşıma sahiptiler. Sham-operasyonlu ve amniyotik membran gruplarında, yara oluşturmak için median hat boyunca 4 cm’lik bir ensizyon yapıldı. Sonrasında, amniyotik membran grubunun abdominal fasiyası, amniyotik membran ile kapatılırken, sham-operasyonlu grubun abdominal fasiyası amniyotik membran eklenmeden kapatıldı. Ondördüncü günün sonunda, hayvanlar öldürüldü ve kan numuneleri alınarak biyokimyasal parametreler yönünden analiz edildi. Tüm plasenta ekstraktlı amniyotik membran; laktat dehidrojenaz (LDH), alanin aminotransferaz (ALP) ve aspartate aminotransferaz; (AST) enzimleri ile Fe, Na ve Cl elementleri yönünden zengindi. Amniyotik membran grubunun C-reactive protein (CRP), LDH ve AST serum ortalama değerleri, belirgin olarak düşük iken, yine bu grubun albumin ve demir serum ortalama konsantrasyonları, sham-operasyonlu grubun değerleriyle karşılaştırıldığında, belirgin olarak daha yüksekti (P

Anahtar Kelimeler:

sıçan, karın, alanin aminotransferaz, antienflamatuar ajanlar, aspartat aminotransferaz, kan kimyası, C-reaktif protein, klorür, enzim aktivitesi, iyileşme, enflamasyon, demir, laktat dehidrogenaz, plasenta, serum albumin, sodyum, doku iyileşmesi, yara

Amniyotik membranın deneysel olarak indüklenmiş steril olmayan temiz yara inflamasyonunda serum biyokimyasal parametreler üzerine etkisi

The effect of amniotic membrane on biochemical parameters was investigated in response to experimentally induced non-sterile clean wound inflammation. In addition, the effort was made to find out the biochemical compounds of human placenta extract. Thirty male Wistar-Albino rats weighing 250-300 g were used and randomly divided into three groups of 10. The control group, subjected to no operation; sham-operated group, subjected to experimental clean wound inflammation; and amniotic membrane group, subjected to experimental clean wound inflammation and received Human Amniotic Membrane (HAM) treatment. All rats had free access to standard laboratory diet and water. In the sham-operated and amniotic membrane groups, a 4 cm-incision was made on the median line in order to create a wound. The abdominal fascia of the amniotic membrane group was then covered with amniotic membrane whilst the abdominal fascia of the sham-operated group was uncovered with a material. At the end of 14 days, the animals were sacrificed and blood samples were obtained and analyzed for biochemical parameters. Amniotic membrane with whole placenta extracts that were rich in enzymes as lactate dehydrogenase (LDH), alanine aminotransferase (ALP) and aspartate aminotransferase (AST), and elements including Fe, Na and Cl. The amniotic membrane group had significantly lower in the mean values of serum C-reactive protein (CRP), LDH and AST whilst this group had significantly higher in the mean concentration of serum albumin and iron compared to sham-operated values (P<0.05). Similarly, the mean values of serum albumin, iron, AST were significantly lower in amniotic membrane group whereas the activity of GGT was higher in this group compared to control values. These results may indicate that amniotic membrane exerts anti-inflammatory effect and may decrease severity of tissue damage and accelerate healing process in rats with experimentally induced non-sterile clean wound inflammation.

Keywords:

rats, abdomen, alanine aminotransferase, antiinflammatory agents, aspartate aminotransferase, blood chemistry, C-reactive protein, chloride, enzyme activity, healing, inflammation, iron, lactate dehydrogenase, placenta, serum albumin, sodium, tissue repair, wounds,

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