SERPİL SARIÖZKAN, FAZİLE CANTÜRK TAN, ARZU HANIM YAY, AYTAÇ AKÇAY

The effect of different storage temperature on sperm parameters and dna damage in liquid stored new zealand rabbit spermatozoa

İki farklı sıcaklığın (4oC ve 15oC), kısa süreli saklamanın 0. ve 24. saatinde tavşan spermatozoasının motilite, plazma membran bütünlüğü, akrozom anomalisi ve DNA hasarı üzerine etkileri değerlendirildi. Altı Yeni Zelanda erkek tavşandan suni vagina yardımıyla alınan ejakülatlar değerlendirmeyi takiben 37°C’de birleştirildi. Birleştirilmiş ejakülat iki eşit kısıma ayrıldı ve Eppendorf plastik tüpde final yoğunluğu yaklaşık 40x106 sperm/ml olacak şekilde Tris bazlı sperma sulandırıcısı ile sulandırıldı. Kısa süreli saklamanın başlangıcında (0. saat’te) 4oC ve 15oC arasında yukarıda bahsedilen parametrelerin oranında önemli bir farklılık yoktu. Saklamanın 24. saatinde, 15oC’de motilite (%75.0±1.83) ve plazma membran fonksiyonel bütünlüğü oranı (%71.2±1.14), 4oC’de kısa süreli saklanan spermanınkinden (%67.9±1.01 ve %65.3±1.38, P

Anahtar Kelimeler:

depolama, tavşan, akrozom, akrozom reaksiyonu, DNA, plazma membranı, semen, semen özellikleri, semen sulandırıcı katkıları, semen sulandırıcısı, semen koruma, spermatozoa, sıcaklık, hücre zarı

Farklı saklama ısılarının kısa süreli saklanan yeni zelanda tavşan spermatozoasının dna hasarı ve sperm parametreleri üzerine etkisi

The effect of two different temperatures (4oC and 15oC) on motility, plasma membrane integrity, acrosome abnormality and DNA damage of rabbit spermatozoa was evaluated at 0 and 24 h of liquid storage. Ejaculates collected from six New Zealand male rabbits by artificial vagina and pooled at 37oC following evaluation. Pooled ejaculate was divided into two equal aliquots and diluted with the Tris based semen extender at a final concentration of approximately 40x106 sperms/ml in a Eppendorf plastic tube. There were no significant differences in the percentage of above mentioned parameters between 4oC or 15oC at the beginning of liquid storage (0 h). The percentages of motility (75.0±1.83%) and plasma membrane functional integrity (71.2±1.14%) at 15oC was significantly better than that of liquid stored semen at 4oC (67.9±1.01% and 65.3±1.38%, P<0.05, respectively) at 24 h of storage. The percentage of acrosome abnormality at 24 h wasn’t affected by the different storage temperature. The influence of storage temperature and the length of time on spermatozoa DNA damage was found statistically significant (P<0.001). The storage period for up to 24 h lead to an increase in the percentage of spermatozoa DNA damage (P<0.001). The percentages of DNA damage at 4oC was statistically higher than 15oC (P<0.001). In conclusion, 15oC may be prefered when liquid stored rabbit semen are used for 24 h.

Keywords:

storage, rabbits, acrosome, acrosome reaction, DNA, plasma membranes, semen, semen characters, semen diluent additives, semen diluents, semen preservation, spermatozoa, temperature, cell membranes,

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