MUSTAFA KEMAL SARIBAY, GÖKHAN DOĞRUER, FİKRET KARACA, CAFER TAYYAR ATEŞ

Effects of long and short-term progestagen treatments plus GnRH followed by taı on fertility parameters in lactating hair goats during the transition period

Çalışma, geçiş döneminde laktasyondaki keçilerde 6 veya 12 gün süreli kullanılan progestagen içeren intra-vaginal sünger ugulamalarının sabit zamanlı tohumlamalardaki etkinliği aynı zamanda sabit zamanlı tohumlamaların GnRH ile kombine edilmesinin fertilite üzerine etkilerini belirlemek amacıyla yürütüldü. Araştırma yaşları 2-5 arasında değişen, en az bir doğum yapmış 104 baş Kıl Keçisi üzerinde yürütüldü. Keçilerin tamamına 30 mg fluorogeston asetat (FGA) içeren sünger uygulandı. Süngerler kısa dönem (ST, n=52) grubunda 6 gün, uzun dönem grubunda (LT, n=52) ise 12 gün süreyle vaginada tutuldu. Süngerlerin çıkartıldığı gün keçilere 400 IU PMSG ve 0.075 mg cloprostenol kas içi enjekte edildi. Keçilerin tamamı süngerler çıkartıldıktan 48 saat sonra soğutulmuş taze sperma kullanılarak tohumlandılar. Tohumlama zamanında ST grubundaki keçiler ST1 (n=24) ve ST2 (n=24), LT gruplarındaki keçiler ise LT1 (n=22) ve LT2 (n=23) olmak üzere kendi aralarında rastgele iki alt gruba ayrıldılar. ST1 ve LT1 kontrol grubu olarak bırakıldı, ST2 ve LT2 grubundaki keçilere tohumlama anında 5 mcg buserelin asetat uygulandı. ST1, ST2, LT1 ve LT2 gruplarında sırasıyla gebelik oranları %37.5, %41.6, %40.9, %47.8, ikizlik oranları %22, %30, %11, %18, yavru verimleri ise %122, %130, %111, %118 olarak saptandı. Bütün çalışma gruplarında doğum oranları %100 olarak tespit edildi. Gruplar arasında gebelik oranı, doğum oranı, ikizlik oranı ve yavru verimleri arasında farklılık önemli değildi (P>0.05). Çalışmanın sonuçları kıl keçilerinde geçiş döneminde östrüs tespiti yapılmadan uygulanan sabit zamanlı tohumlamalarda hem kısa hem de uzun dönem vaginal sünger uygulamalarının kullanılabileceğini göstermektedir. Buna ek olarak progesteron kullanılarak yapılan östrüs tespitsiz sabit zamanlı tohumlamanın geleneksel östrüs tespiti yapılarak uygulanan suni tohumlama uygulamalarına iyi bir alternatif olabileceği kanısına varıldı. Aynı zamanda tohumlama anında yapılan GnRH uygulamalarının reprodüktif parametreleri artırmadığı kanısına varıldı.

Anahtar Kelimeler:

GnRH, kızgınlık, gebelik, gebelik oranları, progesteron, üreme randımanı, dölverim performansı, üreme özellikleri, eş zamanlama, ikiz doğurma, PMSG, suni tohumlama, kloprostenol, döl verimi, flugeston, keçi, laktasyon, yavru sayısı

Geçiş döneminde laktasyondaki Kıl Keçilerinde kısa ve uzun süreli progestagen uygulamalarıyla sabit zamanlı suni tohumlamayla birlikte GnRH kullanımının fertilite parametreleri üzerine etkisi

The aim of this study was to determine the efficiency of the progestagen containing intravaginal sponges used for 6 or 12 days at timed artificial insemination (TAI) in Hair Goats during the transition period, the effect of GnRH administrations at the time of TAI on fertility was also investigated. In this study 104 lactating hair goats aged between 2-5 years old minimally primiparous was used as animal materials. All of the goats received 30 mg fluorogestone acetate (FGA) impregnated vaginal sponges. The sponges were inserted into vagina for 6 days in the short-term (ST, n=52) group also for 12 days in the long-term (LT, n=52) group. The goats received 400 IU PMSG and 0.075 mg of cloprostenol at the time of sponge removal. The goats in both the ST and LT groups timed artificially inseminated 48 h after sponge withdrawal with fresh cooled semen. At the time of artificial insemination, the subgroups ST1 (n=24) and LT1 (n=22) were maintained as controls, the subgroups ST2 (n=24) and LT2 (n=23) received 5 mcg of buserelin acetate. Pregnancy and twinning rates of the ST1, ST2, LT1 and LT2 groups were 37.5%, 41.6%, 40.9%, 47.8% and 22%, 30%, 11%, 18%, respectively. The litter sizes were 122%, 130%,111% and 118% in the ST1, ST2, LT1 and LT2 groups, respectively. The parturition rates were 100% in all treatment groups. Pregnancy rates, twinning rates and litter sizes were not statistically different among the groups (P>0.05). The results of this study indicated that, a TAI protocol without detection estrus seems to be a suitable administration for short and long progesterone sponges aplications in lactating hair goats during the transition period. In addition, progesteron and AI without estrus detection procedure may be useful alternative to traditional synchronization program using progesteron and AI after estrus detection. Also, GnRH addition to progesterone sponges did not improve reproductive paramaters.

Keywords:

GnRH, oestrus, pregnancy, pregnancy rate, progesterone, reproductive efficiency, reproductive performance, reproductive traits, synchronization, twinning, PMSG, artificial insemination, cloprostenol, fertility, flugestone, goats, lactation, litter size,

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