ÖZGECAN KORKMAZ AĞAOĞLU, ÖZKAN ELMAZ, BENGİ ÇINAR KUL, MAHİYE ÖZÇELİK METİN, MUSTAFA SAATCI, Bilal AKTÜZ, Okan ERTUĞRUL

Identification of β-lactoglobulin gene Sacıı polymorphism in Honamli, hair and Saanen goat breeds reared in Burdur vicinity

Bu çalışma Kıl (39), Saanen (41) ve Honamlı (31) keçi ırklarında β-lactoglobulin geninin 7. ekzonunun SacII RFLP polimorfizminin incelenmesi amacıyla yapılmıştır. β-lactoglobulin-SacII polimorfizmi için toplam 111 baş keçi PZR-RFLP (Polimeraz Zincir Reaksiyonu ve Restriksiyon Parça Uzunluk Polimorfizmi) ile genotiplendirilmiştir. İncelenen ırklarda SacII enzim kesimi sonucu iki allel (A ve B) ve üç genotip (AA, BB ve AB) belirlenmiştir. Kıl, Saanen ve Honamlı keçi ırkları için allel frekansları; A alleli için sırasıyla 0.42, 0.37 ve 0.53; B alleli için sırasıyla 0.58, 0.63 ve 0.47 bulunmuştur. Genotipik frekanslar ise AA genotipi için sırasıyla 0.13, 0.12 ve 0.19; BB genotipi için 0.28, 0.39 ve 0.13; AB genotipi için ise; 0.59, 0.49 ve 0.68 bulunmuştur. Çalışılan ırklarda Hardy-Weinberg dengesinden sapma görülmemiştir. Sonuç olarak, bu çalışmada üç keçi ırkında β-lactoglobulin gen polimorfizmi hakkında bilgi verilmiştir. Ayrıca, bu çalışma ile Honamlı keçisinde β-lactoglobulin gen polimorfizmi varlığı ilk defa bildirilmiştir.

Anahtar Kelimeler:

genotip, ekzon, genetik polimorfizm, genetik kararlılık, genler, gen frekansı, kıl keçisi, kısıtlı fragment uzunluğu polimorfizmi, ırk farklılıkları, beta-laktoglobulin, alleller, Saanen, DNA, DNA/RNA kesme enzimleri, polimeraz zincir reaksiyonu, keçi, tanı, keçicilik işletmeleri, keçi ırkları

Burdur ili ve civarında yetiştirilen Honamlı, kıl ve Saanen keçi ırklarında β-lactoglobulin geni Sacıı polimorfizminin belirlenmesi

This study was conducted to determine DNA-polymorphism of a SacII RFLP at exon 7 of the β-lactoglobulin (β-LG) gene in Honamli (31), Hair (39) and Saanen (41) goat breeds. A total of 111 goats were genotyped for the β-lactoglobulin-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the studied breeds, digestion of amplification product with SacII restriction enzyme revealed two alleles namely, A and B and three genotypes (AA, BB and AB). Allelic frequencies for Hair, Saanen and Honamli breeds were 0.42, 0.37 and 0.53 respectively for A allele; 0.58, 0.63 and 0.47 respectively for B allele, while genotypic frequencies were 0.13, 0.12 and 0.19 for AA, 0.28, 0.39 and 0.13 for BB, and 0.59, 0.49 and 0.68 for AB respectively. Deviation from Hardy-Weinberg equilibrium was not observed in the studied breeds. As a result, this study provided information on the polymorphism of β-lactoglobulin in three goat breeds. Additionally, this study reported the existence of a genetic polymorphism at β-LG gene in Honamli goat breed for the first time.

Keywords:

genotypes, exons, genetic polymorphism, genetic equilibrium, genes, gene frequency, hair goat, restriction fragment length polymorphism, breed differences, beta-lactoglobulin, alleles, Saanen, DNA, restriction endonucleases, polymerase chain reaction, goats, identification, goat keeping, goat breeds,

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