Bazı Gıdalarda Kafein ve Teobromin Düzeyleri

Araştırmada, piyasadan satın alınan çözünür kahve, çözünür kakao tozu ve kolalı içecekler kullanılmıştır. Çözünür kahveden 11 kafeinli örnek, 6 kafeini azaltılmış örnek; çözünür kakao tozundan 8 örnek; normal kolalı içecekten 14 örnek, diyet kolalı içecekten 5 örnek olmak üzere toplam 44 örnek, diyot arraydedektörlüHPLC ile analiz edilmiştir. Çözünür kahvenin kafeinli örneklerinde kütlece % 2.687-5.400, kafeini azaltılmış örneklerinde kütlece % 0.110-0.207 arasında kafein bulunmuş olup, teobromin bulunamamıştır. Toz kakao örneklerinde kütlece % 0.140-0.177 arasında kafein, kütlece % 1.857 - 2.190 arasında teobromin saptanmış olup, teobromin/kafein oranı 10.98:1 - 15.30:1 arasında bulunmuştur. Kolalı içeceklerin normal örneklerinde 52,23 ile 117,82 mg/L arasında, diyetlerde 94,080 -139,02 mg/L arasında kafein saptanmış, teobromin her iki kolalı örnekte de bulunamamıştır.

Anahtar Kelimeler:

Kafein, teobromin, HPLC, çözünür kahve, çözünür kakao, kolalı içecekler

Caffeine and Theobromine Levels in Selected Foods (Turkish with English Abstract)

In this study, 11 samples of regular instant coffee, 6 samples of decaffeinated instant coffee, 8 samples of instant cocoa powder, 14 samples of carbonated beverage and 5 samples of diet carbonated beverage, totally 44 samples, purchased from the markets, were analysed for caffeine and theobromine contents using HPLC-DAD. No theobromine werededected in instant and decaffeinated coffee samples while caffeine was determined in the range of 2,689-5,400 % and 0,110-0,207 % respectively. In instant cocoa samples, 0,140-0,177 % caffeine and 1,857-2,190 % theobromine were dedected and the theobromine/caffeine rate was calculated between 10,98:1-15,30:1. Caffeine contents were between 52,23-117,82 mg/L in carbonated beverage samples and between 94,080-139,02 mg/L in diet carbonated beverage samples whereas no theobromine was dedected in both.

Keywords:

Caffeine, theobromine, HPLC, instant coffee, instant cocoa, carbonated beverages. ,

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