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Hardaliyenin HT-29, DU-145, HeLa Kanser Hücreleri ve CF-1 (Fare Embriyonik Fibroblast) Hücresi FoxM1 Gen Expresyon Seviyelerine Etkisi

Kemoterapötik ajanlar hem kanser hücrelerini hem de sağlıklı hücreleri etkilemektedir bu nedenle alternatif terapötik ajanlar veya hedefler kanser tedavisinin kilometre taşı olmuştur. Forkhead box M1 (FoxM1) transkripsiyonel regülatördür ve kanser hücrelerinde daha fazla eksprese olması nedeniyle yeni kanser terapi hedefi olmuştur. Fox M1 inhibitörleri, kanser gelişimini engelleyen potansiyel terapötik ajanlardır. Bu nedenle, çalışmada, Hardaliye'nin kanser hücreleri ve sağlıklı hücre hattı üzerine etkisi MTT yöntemiyle öncelikle belirlenmiş ve sonrasında, FoxM1 gen ekspresyon seviyeleri tayin edilmiştir. Hardaliye, özellikle on ve yirmi-kat seyreltildiğinde bütün kanser hücrelerinin yüzde canlılığını düşürmüş ancak sağlıklı hücreleri etkilememiştir. FoxM1 seviyeleri sağlıklı hücre hatlarında istatistiksel olarak değişmezken, kanser hücrelerinde özellikle HT-29 hücrelerinde epeyce düşmüştür.

Effect of Hardaliye on FoxM1 Gene Expression Level of HT-29, DU-145, HeLa Cancer Cells and CF-1 (Mouse Embryonic Fibroblast)

Chemotherapeutic agents may influence both cancer cells and healthy cells, therefore alternative therapeutic agents or targets have become a milestone of cancer cure. Forkhead box M1 (FoxM1) is a transcriptional regulator and a novel cancer therapy target as overexpressed in cancer cells, and its inhibitors are considered as potential therapeutic agents to halt cancer progression. Therefore, in this study, the effect of "Hardaliye" on cancer cells and a healthy cell line was primarily investigated by MTT (3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide) and later on, FoxM1 gene expression levels were determined. Hardaliye, especially when diluted ten and twenty-fold, decreased viability percentage of all cancer cells and did not affect healthy cells. FoxM1 levels of cancer cells drastically decreased especially in HT-29 cells while did not statistically change their levels in the healthy cell line.

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