Grafen Oksit-Polianilin Nanokompozit Temelli Amperometrik Glukoz Biyosensörü Geliştirilmesi

Bu çalışmada, Pt elektrot, grafen oksit-polianilin (GO-PANI) nanokompoziti ile modifiye edilerek amperometrik glukoz biyosensörü geliştirilmesinde kullanılmıştır. Modifiye edilmiş Pt elektroda glukoz oksidaz (GOD) enzimi çapraz bağlayıcı kullanılarak immobilize edilmiştir. Bu şekilde hazırlanan Pt/GO-PANI/GOD biyosensörünün optimum çalışma koşullarında amperometrik yöntemle glukoza karşı cevabı çalışılmış ve 36.11 ?A mM-1 cm-2 hassasiyet, 0.59-9.15 mM lineer çalışma aralığı ve 0.24 mM tayin limiti sergilemiştir. Geliştirilen glukoz biyosensörü üzerine askorbik asit (AA) ve ürik asidin (UA) girişim etkileri incelenmiş ve biyosensörün glukoza karşı oldukça seçici olduğu gözlenmiştir.

Grafen Oksit-Polianilin Nanokompozit Temelli Amperometrik Glukoz Biyosensörü Geliştirilmesi

In this study, Pt electrode was modified with graphene oxide-polyaniline (GO-PANI) nanocomposite and used in the fabrication of amperometric glucose biosensor. Glucose oxidase (GOD) was immobilized on the modified Pt electrode by using a cross-linking agent. The proposed Pt/GO-PANI/GOD biosensor exhibited a sensitivity value of 36.11 ?A mM-1 cm-2with a linear range of 0.59-9.15 mM and the detection limit of 0.24 mM under optimum conditions. The interference effects of ascorbic acid (AA) and uric acid (UA) on the developed glucose biosensor were studied, and it was observed that the biosensor was highly selective to glucose.

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