Horozlarda üropigi bezinde IGF-I reseptörünün immunohistokimyasal yerleşimi

Çalışmanın amacı, hücre gelişmesi ve farklılaşmasında önemli rol oynayan insülin benzeri büyüme faktör -1 reseptörünün (IGF-IR), horozların üropigi bezindeki dağılımını belirlemek ve reseptörün hücre içindeki yerleşim yerinin belirlenmesi amacıyla planlanmıştır. Üropigi bezinden elde edilen parafin bloklara, indirekt Streptavidin-Biotin Peroksidaz immunohistokimyasal yöntemi uygulandı. Çalışmada IGF-I reseptörünün üropigi bezinin bazal ve apikal bölgesindeki hücrelerde eksprese olduğu gözlendi. Özellikle bazal bölgede yer alan tubulusların bazal membran üzerine yerleşen hücrelerinde IGF-I reseptör ekspres-yonu çok yoğun olarak belirlendi. IGF-I reseptör, üropigi epitel hücrelerinin sitoplazmasında, granül halinde gözlendi. Sonuçlarımıza göre; üropigi bezinde çoğalma ve farklılaşma özelliğindeki hücrelerde IGF-IR ekspresyonunun çok yüksek olduğu, tamamen farklılaşmış hücrelerde ise bu reseptörün eksprese olmadığı sonucuna varılabilinir.

Anahtar Kelimeler:

horoz, epitel, biyokimyasal reseptörler, insulin benzeri büyüme faktörü, immünohistokimya, kümes hayvanları, deri bezleri

Immunohistochemical localization of insulin like growth Factor-I receptors in the uropygial gland of cockrell

The objective of the present study was to determine the distribution and intracellular localization of insulin-like growth factor-1 receptor (IGF-IR), that plays a very important role in the differentiation and growth of the cells, in the uropygial gland of cockrell. We applied Labelled Streptavidin Biotin immunohistochemistry to paraffin sections to show the localization of IGF-IR. IGF-I receptor was found in the cells, of both basal region and apical region. Particulary, basal cells, which lie on the basement membrane of the tubules in the basal region, showed the strongest immunoreactivity to the IGF-I receptor antibody. In our study, IGF-I receptors showed a granular localization in the cytoplasm of uropygial epithelial cells. Our results show that IGF-I receptors are widely expressed by epithelial cells with proliferative and undifferentiated and that these receptors may be largely down-regulated in highly differentiated epithelial cells.

Keywords:

cocks, epithelium, biochemical receptors, insulin-like growth factor, immunohistochemistry, poultry, skin glands,

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