Epidermal büyüme faktörü reseptörünün (EGFR) horozların üropigi bezinde immunohistokimyasal yerleşimi

Epidermal büyüme faktörü (EGF), düşük moleküler ağırlıklı bir polipeptitdir. Epidermal büyüme faktörü, reseptöre (EGFR) bağlanarak hücrelerde bölünme ve farklılaşmayı uyarır. Çalışma epidermal büyüme faktör reseptörünün hücre bölünme ve farklılaşmasının fazla olduğu üropigi bezindeki dağılımı ve reseptörün hücre içi yerleşim yerinin belirlenmesi amacıyla planlandı. Üropigi bezinden elde edilen parafin bloklara indirekt Avidin-Biotin Peroksidaz immunohistokimyasaj yöntem uygulandı. Çalışmada EGFR'nin, özellikle bezin bazal bölgesinde yer alan tubulusların bazal membran üzerine yerleşen hücrelerinde fazla, lumene doğru yer alan diğer hücrelerde ise, çok az eksprese olduğu, apikal bölgedeki tubulusların duvarını oluşturan tüm hücrelerde ise EGFR ekspresyonunun orta şiddette olduğu belirlendi. Sunulan çalışmada bazal hücrelerde EGFR ekspresyonunun yüksek seviyede gözlenmesi bu hücrelerin sık mitoz aktivite gösterdiği, glikojen bölgesi olarak tanımlanan, apikal bölgedeki tüm hücrelerde EGFR ekspresyonunun orta şiddette olması, EGFR'nin bu hücrelere glukoz transportunda rol oynadığını akla getirmektedir.

Anahtar Kelimeler:

deri bezleri, doku dağılımı, hücre farklılaşması, immünohistokimya, kümes hayvanları, epidermal büyüme faktörü, biyokimyasal reseptörler, horoz

Immunohistochemical localization of epidermal growth factor receptors (EGFR) in the uropygial glands of cokrell

Epidermal growth factor (EGF) is a low molecular weight polypeptide. EGF stimulates cell proliferation and differentiation by binding to its reseptors (EGFR).The aim of the present study was to determine the distribution and intracellular localization of epidermal growth factor receptor (EGFR) in the uropygial gland in which cell proliferation and differentiation is present. We applied Labelled Avidin Biotin Peroxidase immunohistochemistry method to paraffin sections to show the localization of EGFR. In this study, particulary, the basal cells, which lie on the basement membrane of the tubules in the basal region, showed the strongest expression, the other cells towards the lumen of the tubule showed slightly expression to the EGFR antibody. Medium EGFR expression was observed in all cells of the apical region. According to our results, strong EGFR expression in the basal cells show that these cells are mitogenic. The medium EGFR expression in all of the cells of apical region which is known as glikogen region supposes us that EGFR may have effects on glucose transportation.

Keywords:

skin glands, tissue distribution, cell differentiation, immunohistochemistry, poultry, epidermal growth factor, biochemical receptors, cocks,

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