Yetiştirme Ortamlarının Hericium İzolatlarının Verim ve Şapka Özellikleri Üzerine Etkisi

Bu çalışmada, farklı yetiştirme ortamlarının 7 Hericium izolatının (HE-Ankara, HE- Denizli, HE, HE-Trabzon, HE-İzmit, HC ve HE-Amerika) misel gelişimi, verim, şapka boyutları ve rengi üzerine etkileri araştırılmıştır. Denemede, meşe talaşı (MT) ile buğday kepeği (BK), pamuk küspesi (PK) ile hazırlanan 7 farklı yetiştirme ortamı (80MT:20BK, 90MT:10PK, 80MT:20PK, 70MT:30PK, 90MT:10ZP, 80MT:20ZP, 70MT:30ZP) ele alınmıştır. Hazırlanan ortamlar 29 x 45 cm boyutlarındaki ısıya dayanıklı polipropilen torbalara doldurulmuş ve 121oC'de 90 dakika sterilize edilmiştir. Sterilize edilen ortamlara misel ekimi yapılarak, torbalar 25±2oC'deki misel gelişim odalarına yerleştirilmiştir. Misel gelişimi tamamlandıktan sonra, 20±2oC sıcaklık ve %80-90 nisbi neme ayarlanmış üretim odalarında günde 12 saat 400 lüks'lük ışıklandırma yapılmıştır. Denemelerde HE-Ankara, HE-Denizli, HE, HE-İzmit, HC, HE-Amerika izolatlarında en yüksek verim ve BE %20 ve 30 oranında PK eklenen ortamlarda alınır iken, HE-Trabzon izolatında ise en yüksek verim ve BE 70MT:30ZP ortamında belirlenmiştir. En düşük verim ise tüm izolatlarda 80MT:20BK ortamında elde edilmiştir. Ayrıca farklı yetiştirme ortamlarının Hericium izolatlarının ortalama mantar ağırlığı, şapka boyutları ve rengi üzerindeki etkileri de %1 düzeyinde önemli bulunmuştur

Effect of Growing Media on Yield and Fruitbody Properties of Hericium Isolates

In the present study, it was aimed to determinate the effects of different substrates on mycelial growth, fructification, yield, sizes and colours of fruit bodies of Hericium isolates (HE-Ankara, HE-Denizli, HE, HE-Trabzon, HE-İzmit, HC, HE-Amerika). Experiments were conducted with 7 different substrates prepared with oak sawdust (MT), wheat bran (BK), cotton seed hulls (PK) and olive press cake (ZP) (80MT:20BK, 90MT:10PK, 80MT:20PK, 70MT:30PK, 90MT:10ZP, 80MT:20ZP, 70MT:30ZP). 1 kg (wet weight) of substrates were packed in polypropylene autoclaveable bags of 25x45 cm and sterilized in autoclave at 121oC during 90 minutes. Sterilized substrate was inoculated and then carried to growing room at 25±2ºC. After full colonization, the bags were exposed to 400 lux for a 12 hours photoperiod at 20±2ºC with a humidity of 80-90% in a cropping room. The best yield and BE were detected from oak sawdust medium supplemented with 20 and 30% cotton seed hulls on HE-Ankara, HE-Denizli, HE, HE- İzmit, HC, HE-Amerika isolates while the best yield and BE were detected from 70MT:30ZP on Trabzon isolate. Significant differences were found among substrates regarding yield, BE, average mushroom weigh, fruit body size and colour of Hericium isolates.

___

  • Akdeniz H. 2012. Değişik tarımsal atıkların Hericium erinaceus mantar üretimimde kullanım olanakları, Yüksek Lisans Tezi, Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, 53 s (yayımlanmamış).
  • Carvalho CSM, Sales-Campos C, De Andrade MCN. 2010. Mushrooms of the Pleurotus Genus: A review of cultivation techniques. Interciencia, 35 (3): 177-182.
  • Chang SH, Miles PG. 2004. Mushrooms Cultivation, Nutritional Value, Medicinal Effect and Environmental Impact, 385 p.
  • Choi KY, Nair NG., Bruniges PA. 1981. The use of cotton seed hulls for cultivation of P. sajor-caju in Australia. Mushroom Science. 11: 679-690.
  • Cormican T, Stauton L. 1991. Factors in mushroom (Agaricus bisporus) compost producity. In: MAHER, M.J. (Ed.) Science and cultivation of edible fungi. Rotterdam: Balkema 1: 223-234.
  • Dhar BL. 1994. Mushroom composting for Agaricus bisporus/bitorquis. In: NAIR, M.C. (Ed.) Advances in mushroom biotechnology, Jophpur: Scientific Publishers, 84-90 p.
  • Ehlers S, Schnitzler W. 2000. Studies on the growth of the basidomycete Hericium erinaceusn (Bull.Ex.Fr.) Pers., Champignon, 415: 147-150.
  • Figlas, D, Matute, RG, Curvetto, N, 2007. Cultivation of Culinary-Medicinal Lion's Mane Mushroom Hericium erinaceus (Bull.: Fr.) Pers. (Aphyllophoromycetideae) on Substrate Containing Sunflow~r Seed Hulls. International Journal of Medicinal Mushrooms, 9: 67-73.
  • Gerrits JPG. 1988. Nutrition and compost. In: Griensven, L. J. L. D.(Ed.) The cultivation of mushrooms. Horst: Mushroom Experimental Station, 29-72 p
  • Hassan FRH. 2007. Cultivation of the Monkey Head Mushroom (Hericium erinaceus) in Egypt. Journal of Applied Sciences Research, 3 (10): 1229-1233.
  • Hernandez RG, Salmones D. 2008. Obtaining and characterizing Pleurotus ostreatus strains for commercial cultivation under warm environmental conditions, Scientia Horticulturae, 118: 106-110
  • Jadhav AB, Bagal PK, Jadhav SW. 1996. Effect of different substrates on yield of oyster mushroom. J. Maharashtra Agriculture University,21 (4): 424-426.
  • Kalyoncu F, Kalmış E. 2007. Pirinanın farklı Pleurotus türlerinin araştırılması. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9 (2): 87-92. kullanım olanaklarının
  • Khare, KB, Mutuku, JM, Achwania, OS, Otaye, DO. 2010. Production of two oyster mushrooms, Pleurotus sajor-caju and P. florida on supplemented and un-supplemented substrates. Botswana. Journal of Agriculture and Applied. Science, 6: 4-11.
  • Ko HG, Park HG, Park SH , Choi CW, Kim SH , Park WM. 2005. Comparative study of mycelial growth and basidiomata formation in seven different species of the edible mushroom genus Hericium. Bioresource Technology, 96: 1439-1444.
  • Lavie D. 1988. Production of oyster mushroom on cotton straw. Mushroom Journal, 82: 453-463.
  • Mane VJ, Patil SS, Syed AA, Baig MMV. 2007. Bioconversion of low quality lignocellulosic agricultural wastes into edible protein Pleurotus sajor-caju (Fr.) Singer J. Zhejiang. University. Science B, 8: 745-751.
  • Nout MJR, Keya SO, 1983. Cultivation of Pleurotus sajor-caju in Kenya. Mush. Newslett. Trop. 4 (2): 12-15
  • Nwanze PI, Khan AU, Ameh JB, Umoh VJ. 2004. The effect of the interaction of various spawn grains with different oil rates on carpophore wet weights and stipe and pileus diameters of Psathyrella atroumbonata. International. Journal of. Science. Technology. Research. 1(1-2): 103-111.
  • Nwanze PI, Ameh JB, Umoh VJ. 2005. The effect of the interaction of various oil types with different culture media on biomass production of Psathyrella atroumbonata Pegler, African Journal of Biotechnology, 4 (11): 1285-1289,
  • Oei P. 1996. Mushroom cultivation. Manual on mushroom cultivation. Tech. commercial application in developing countries. Tools publications. Amsterdam, 94-119 p.
  • Onyango BO, Palapala VA, Arama PF, Wagai SO, Gichumu BM. 2011. Sustainability of selected supplemented substrates for cultivation of Kenyan native wood ear mushrooms (Auricularia auricula). American. Journal of Food Technolology, 6: 395-403.
  • Oseni, TO, Dube SS, Wahome PK. 2012. Masarirambi, M.T. Earnshaw, D.M., Oyster Mushroom (Pleurotus Ostreatus) on Fermented Agriculture and Horticulture, 30-40 p Substrate. Experimental
  • Parades C, Cegarra J, Roig A, Sanchez-Monedero MA, Bernal MP. 1999. Characterization of olive mil wastewater and its sludge for agricultural purposes. Bioresource Technology, 67: 111-115
  • Pekşen A. 2001. Fındık zurufundan hazırlanan yetiştirme ortamlarının Pleurotus sajor-caju mantarının verimine ve bazı kalite özelliklerine etkisi. Bahçe 30 (1-2): 37 - 43
  • Perez JD, Esteban E, Gómez M, Gallardo-Lara F. 1986. Effects of wastewater from olive processing on seed germination and species. Journal of Environmental Science and Health, B, 21 (4): 349-357 of different vegetable
  • Ponmurugan P, Sekhar YN, Sreeshakti TR. 2007. Effect of various substrates on the growth and quality of mushrooms. Pakistan Journal of Biological Science 10: 171-173.
  • Quinio TH, Chang ST, Royce DJ. 1990. Technical guidelines for mushroom growing in the tropics. FAO plant production and protection paper, Rome, 65 p
  • Rodriguez MM, Perez J, Ramos-Cormenzana A, Martinez, J. 1988. Effect of extracts obtained from olive oil mill wastewaters on Bacillus megaterium ATCC33085. Journal of. Applied. Bacterialogy, 64: 219-226.
  • Royse DJ, Rhodes TW, Ohga S, Sánchez JE. 2004. Yield, mushroom size and time to production of Pleurotus cornucopiae (oyster mushroom) grown on switch grass substrate spawned and supplemented at various rates. Bioresource Technology, 91: 85-91.
  • Ruiz-Rodriguez A, Soler-Rivas C, Polonia I, Wichers JH. 2010. Effect of olive mill waste (OMW) supplementation to Oyster mushrooms substrates on the cultivation parameters and fruiting bodies quality. International Biodeterioration and Biodegradation, 64 (7): 638-645
  • Sampedro I, Marinari S, D´Annibale A, Grego S, Ocampo JA, García-Romera I. 2007. Organic matter evolution and partial detoxification in two-phase olive mill waste colonized by white-rot fungi. International Biodeterioration and Biodegradation, 60: 116-125.
  • Sharma AD. 1983. Effect of cropping environment on some quality parameter of oyster mushroom Pleurotus sajor- caju. MushroomScience.,4: 1-5.
  • Shen Q, Royse DJ. 2001. Effects of nutrient supplements on biological efficiency, quality and crop cycle time of maitake (Grifola frondosa). Applied Microbiology Biotechnology, 57 : 74-78
  • Siwulski M, Sobieralski K. 2005. Influence of some growing substrate additives on the Hericium erinaceus (Bull. Fr.) Pers. yield. Sodininkyste ir Darzininkyste , 24 (3): 250-253.
  • Tomati U, Galli E, Dilena G, Buffone R. 1991. Induction of lactase in Pleurotus ostreatus mycelium grown in olive mill waste waters. Agrochimica, 35: 275-279.
  • Wang Q, Li BB, Li H, Han JR. 2010. Yield, dry matter and polysaccharides content of themushroom Agaricus blazei produced on asparagus straw substrate. Scientia Horticulture, 125: 16-18.
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)