Tunceli Dağ Sarımsağının ( Allium tuncelianum ) Rat Kalp Dokusu Antioksidan Enzim Düzeylerine Etkisi ve Fenolik Bileşenlerinin Karakterizasyonu
Bu çalışmada, Allium tuncelianum'un su ekstraktlarının, fenolikbileşikleri LC-MS/MS ve HPLC ile belirlenmiştir. Antioksidan enzimdüzeylerine etkisini belirlemek için Wistar albino tipi dişi sıçanlarkullanılmıştır. Her grupta 8 adet (n= 8) hayvan içeren 5 grupoluşturuldu.Birincigrupkontrol,ikincigrup7,12Dimethylbenz[a]anthracene (DMBA) , üçüncü ve dördüncü grupDMBA + 250 ve 500 mg/kg/gün A.tuncelianum, beşinci grup DMBA +200 mg/kg E vitamini (haftada iki kez) verilmiştir. Deney hayvanlarıbir ay süresince beslenmiş ve dekapite edilerek kalp dokusualınmıştır. Allium tuncelianum'un su ekstraktının kalp dokusuüzerine antioksidan ve oksidatif stres markırı olan; katalaz (CAT) vesüpeoksit dismutaz (SOD), malondialdehit (MDA) ve total glutatyon(GSH) parametreleri incelenmiştir. Bu çalışma sonucunda Alliumtuncelianum'un su ekstraktının fenolik bileşik çeşitliliği açısındanfakir ancak diğer bazı bileşenler (pirokatekol, kainik asit, fumarik asitve malik asit) açısından oldukça zengin bir içeriğe sahip olduğubulunmuştur. Kalp dokusunda antioksidan enzim (CAT, SOD)düzeylerinin oksidatif stres oluşturulan gruplarda, kontrol gruplarınagöre anlamlı bir şekilde (p
Effect of Tunceli Mountain Garlic ( Allium tuncelianum ) on Rat Heart Tissue Antioxidant Enzyme Levels and Characterization of Phenolic Components
In this study, the phenolic compounds of Allium tuncelianum water extracts were determined by LC-MS / MS and HPLC. Wistar albino female rats were used to determine the effect on antioxidant enzyme levels. Five groups of female rats, each containing eight individuals were studied. The first group was control without any treatments. The second group was treated 7,12-DMBA, third, and fourth groups were treated with DMBA + 250, and 500 mg/kg/day of Allium tuncelianum water extracts, fifth group was treated DMBA+ 200 mg/kg of E vitamin (twice a week) for one month, respectively. The rats were feed for one month, slaughtered afterwards and the cardiac tissue was taken for the experiments. Catalase (CAT) and superoxide dismutase (SOD), Malondialdehide (MDA) and Glutatyon (GSH) parameters, which are antioxidant and oxidative stress markers on the heart tissue of Allium tuncelianum water extract were investigated. Result of this study indicated that Allium tuncelianum water extract was poor in phenolic compound diversity but had a very rich content in terms of some other components including pyrocatechol, kainic acid, fumaric acid and malic acid. Antioxidant enzymes (CAT, SOD) levels in heart tissue decreased significantly (p
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- Akerboom TPM, Sies H 1981. Assay of Glutathione,
Glutathione Disulfide, and Glutathione Mixed
Disulfides in Biological Samples. Methods in
Enzymology, 77(C): 373-382.
- Aras A 2016. Türkiye’de Yetişen Endemik ( Nepeta
nuda Subsp. L.) Bitkisine Ait Farklı Ekstrelerin
Antioksidan Aktivitelerinin Belirlenmesi ve
Fenolik Bileşikiçeriklerinin LC-MS/MS ile Analizi.
D.Ü. Fen Bil. Ens., Kimya ABD, Doktora Tezi, 129
s.
- Banerjee SK, Sood S, Dinda, AK, Das TK, Maulik SK
2003. Chronic oral administration of raw garlic
protects against isoproterenol-induced myocardial
necrosis in rat, Comparative Biochemistry and
Physiology-C. Toxicology and Pharmacology,
136(4): 377-386.
- Bilici M, Efe H, Köroğlu MA, Uydu HA, Bekaroğlu M,
Değer O 2001. Antioxidative enzyme activities and
lipid peroxidation in major depression: alterations
by antidepressant treatments. Journal of Affective
Disorders, 64(1): 43-51.
- Borek C 2001. Antioxidant health effects of aged garlic
extract. The Journal of Nutrition, 131(3): 1010-5.
- Bradford MM 1976. A rapid and sensitive method for
the quantitation of microgram quantities of protein
utilizing the principle of protein-dye binding.
Analytical Biochemistry, 72(1-2): 248-54.
- Dinis TC, Madeira VM, Almeida LM 1994. Action of
phenolic derivatives (acetaminophen, salicylate,
and 5-aminosalicylate) as inhibitors of membrane
lipid peroxidation and as peroxyl radical
scavengers. Archives of Biochemistry and
Biophysics, 315(1): 161-169.
- Eşrefoğlu M, Gül M, Ateş B, Batçıoğlu K, Selimoğlu
MA 2006. Antioxidative effect of melatonin,
ascorbic acid and N-acetylcysteine on caerulein-
induced pancreatitis and associated liver injury in
rats. World Journal of Gastroenterology, 12(2): 259.
- Evans C, Miller N, Paganga G 1997. Antioxidant
properties of phenolic compounds. Trends in Plant
Science, 2(4): 152-9.
- Farag MA, Ali SE, Hodaya RH, El-Seedi HR, Sultani
HN, Laub A, Eissa TF, Abou-Zaid FO, Fratianni F,
Ombra MN, Cozzolino A, Riccardi R, Spigno P,
Tremonte P, Coppola R, Nazzaro F 2016. Phenolic
constituents, antioxidant, antimicrobial and anti-
proliferative activities of different endemic Italian
varieties of garlic ( A. sativum L.). Journal of
Functional Foods, 21: 240-8.
- Geleijnse JM, Launer LJ, Hofman A, Pols HA, Witteman JC 1999. Tea flavonoids may protect
against atherosclerosis: the Rotterdam Study.
Archives of Internal Medicine, 159(18): 2170-4.
- Gülçin I, Küfrevioǧlu Öİ, Oktay M, Büyükokuroǧlu ME
2004. Antioxidant, antimicrobial, antiulcer and
analgesic activities of nettle ( Urtica dioica L.).
Journal of Ethnopharmacology, 90(2-3): 205-15.
- Halliwell B, Chirico S 1993. Lipid peroxidation: its
mechanism, measurement, and significance. The
American Journal of Clinical Nutrition, 57(5): 715-
24.
- Işık M, Korkmaz M, Bursal E, Gulcin I, Koksal E,
Tohma H 2015. Determination of antioxidant
properties of Gypsophila bitlisensis bark.
International Journal of Pharmacology, 11(4): 366-
71.
- İzol E 2016. Bazı Allium (Yabani Sarımsak) Türlerinin
Aflatoksin, Ağır Metal ve Sekonder Metabolit
İçeriklerinin
ICP-MS
ve
LC-MS/MS
İle
Belirlenmesi
ve
Biyolojik
Aktivitelerinin
İncelenmesi. D.Ü. Fen Bil. Ens., Kimya ABD,
Yüksek Lisans Tezi, 129 s.
- Karaoğul E, Kirecci E, Alma MH 2016. Determination
of phenolic compounds from Turkish kermes oak
( Quercus coccifera L.) roots by high performance
liquid chromatography; its antimicrobial activities.
Feb-Fresenius Environmental Bulletin, 25(7):
2356–2363.
- Köksal E, Tohma H, Kılıç Ö, Alan Y, Aras A, Gülçin İ,
Bursal E 2017. Assessment of antimicrobial and
antioxidant activities of Nepeta trachonitica :
analysis of its phenolic compounds using HPLC-
MS/MS. Scientia Pharmaceutica, 85(2): 1-14.
- Kumar VM, Henley AK, Nelson CJ, Indumati O, Rao
YP, Rajanna S, Rajanna B 2017. Protective effect of
Allium sativum (garlic) aqueous extract against
lead-induced oxidative stress in the rat brain, liver,
and kidney. Environmental Science and Pollution
Research, 24(2):1544-52.
- Küçükkurt İ, Acaröz DA, Demirel HH, Sinan İN,
Eryavuz A 2017. Ratlarda Gentamisin İle
İndüklenmiş Oksidatif Streste Borun Muhtemel
Koruyucu Etkisinin Dokularda Araştırılması.
Kocatepe Veteriner Dergisi, 10(3):172-9.
- Landis GN, Tower J 2005. Superoxide dismutase
evolution and life span regulation. Mechanisms of
Ageing and Development, 126(3):365-79.
- Martins N, Petropoulos S, Ferreira IC 2016. Chemical
composition and bioactive compounds of garlic ( Allium sativum L.) as affected by pre-and post-
harvest conditions: A review. Food Chemistry,
211(3):41-50.
- Pandey M, Debnath M, Gupta S, Chikara SK 2011.
Phytomedicine: An ancient approach turning into
future potential source of therapeutics. Journal of
Pharmacognosy and Phytotherapy, 3(1):113-7.
- Solmaz, FÖK 2011. 7,12-DMBA ile İndüklenen Rat
Karaciğer
Dokusunda
Çeşitli
Sarimsak
Ekstrelerinin Koruyucu Etkilerinin İncelenmesi.
İ.Ü. Sağ. Bil. Ens., Biyokimya ABD, Bitirme Tezi,
113 s.
- Takım K 2015. Tunceli Dağ Sarımsağı'nın ( Allium
tuncelianum ) in vitro Antioksıdan Kapasitesinin
Ölçülmesi,
Ratlarda
Antioksidan
Enzim
Aktiviteleri Üzerine Etkisi Ve Antikanser
Özelliğinin Belirlenmesi. İ.Ü. Fen Bil. Ens., Kimya
ABD, Doktora Tezi, 113 s.
- Tello S, Halifeoğlu İ, Bozkurt M, Bulmuş Ö 2008.
Meme Kanseri Oluşturulmuş Ratlarda Isırgan
Otunun Total Antioksidan Durumu Üzerine Etkisi.
Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi. 22 :
179-83.
- Vlase L, Parvu M, Parvu EA, Toiu A 2012. Chemical
constituents of three Allium species from Romania.
Molecules, 18(1):114-27.
- Vardi N, Parlakpinar H, Ozturk F, Ates B, Gul M,
Cetin A, Erdogan A, Otlu A 2008. Potent protective
effect of apricot and β-carotene on methotrexate-
induced intestinal oxidative damage in rats. Food
and Chemical Toxicology, 46(9): 3015-22.
- Yılmaz MA 2015. Bazı Achillea türlerinin LCMS-
IT/TOF ve LC-MS/MS ile metabolik profillerinin
çıkarılması ve biyolojik activitelerinin belirlenmesi.
D.Ü. Fen Bil. Ens., Kimya ABD, Doktora Tezi, 314
s.
- Yünlü S 2011. Soğan ( Allium cepa ) Ve Sarımsaktaki
( Allium sativum ) Fenolik Bileşiklerin HPLC
Yöntemiyle Tayin Edilmesi. S.D.Ü. Fen Bil. Ens.,
Kimya ABD, Yüksek Lisans Tezi, 101 s.
- Wessjohann LA 2017. Phytochemical profiles and
antimicrobial activities of A. cepa red cv. and A.
sativum subjected to different drying methods: a
comparative MS-based metabolomics. Molecules,
22(5): 761.
- Wu CC, Sheen LY, Chen HW, Tsai SJ, Lii CK 2001.
Effects of organosulfur compounds from garlic oil on
the antioxidation system in rat liver and red blood
cells. Food and Chemical Toxicology, 39(6):563-9