Önemli Bir Ekvator Yem Bitkisi Olan Indigofera zollingerian Miq.’nın Çoklu Sürgün Rejenerasyonu

Indigofera zollingeriana , Endonezya'da yaygın olarak yetişen proteince zengin yem ve boya bitkisidir. Bitkiyi tohumdan çoğaltmak yüksek dormansi sebebiyle zor olmaktadır. Bu nedenle, I. zollingeriana 'nın in vitro ve ex vitro koşulllarda çoğaltma tekniklerinin geliştirilmesi gerekmektedir. Bu çalışmada, farklı kombinasyonlarda BAP + 0.01 mg/L NAA içeren MS ortamı kullanılarak in vitro koşullar altında bitkinin hızlı çoğaltım için bir protokol geliştirmesi amaçlanmıştır. En fazla sürgün rejenerasyonu epigeal ve kotiledon nodlarının sırasıyla 1.5 mg / L BAP + 0.01 mg / L NAA ve 1.0 mg/L BAP + 0.01 mg/L NAA içeren MS ortamında elde edilmiştir. BAP + NAA'nın tüm kombinasyonlarında rejenerasyonu yapılan sürgünler 0.5 mg/L IBA kullanılarak başarılı şekilde köklendirilmiştir. Köklü bitkiler, bir iklim dolabında dış koşullara alıştırılmıştır. Bu çalışma I. zollingeriana bitkisinin in vitro koşullar altında yetiştirilebileceğini göstermiş olması ve elde edilen sonuçların ilerideki çalışmalarda aktif bileşiklerin biyosentezi ve ekstraksiyonu ile bitki yetiştirilmesi ve çoğaltılmasına yardımcı olacağını düşünülmektedir.

Multiple Shoot Regeneration of an Important Equatorial Forage Plant Indigofera zollingeriana Miq.

Indigofera zollingeriana is an important protein rich forageanddye plant species that grows widely in Indonesia. It is difficult topropagate the plant through seeds due to high dormancy. Therefore,there is need to develop both in vitro and ex vitro propagationtechniques of I. zollingeriana . This study aimed to develop a protocolfor multiple shoot regeneration of the plant under in vitro conditionsusing different combinations of BAP+0.01 mg/L NAA . The resultsshowed that both epigeal and cotyledon node explants have highpotential to regenerate with maximum number of regeneratedshoots noted on the epigeal and the cotyledon node explants usingMS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0mg/L BAP + 0.01 mg/L of NAA in the same order. The shootsregenerated on all combinations of BAP+NAA were rooted using 0.5mg/L IBA that promoted rooting in effective manner. The rootedplants were easily acclimatized to external conditions in anenvironmental chamber. The results suggest that I. zollengrianaplant species is no more difficult to regenerate under in vitroconditions. This research will aid in future studies related tobreeding, propagation, biosynthesis and extraction of activecompounds.

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