Helianthus annuus L. Yapraklarında Tuz Stresi, Bazı Bitki Hormonları ve SNP Uygulamalarının Sinyal Moleküllerine Etkisi
Bu çalışmada tuz stresi, sodyum nitroprussid ve hormonuygulamalarının Helianthus annuus L. cv. Tarsan-1018 ayciçeğibitkisi yapraklarındaki bazı sinyal moleküllerine etkileriaraştırılmıştır. Helianthus annuus L. cv. Tarsan-1018 tohumlarıEdirne Trakya Tarımsal Araştırma Enstitüsü’nden teminedilmiştir. Tohumlar kültür çözeltisi ile 5 hafta boyuncasulanmıştır. Beşinci haftanın sonunda tuz, sodyum nitroprussid vehormon uygulamaları 72 saat boyunca yapraktan uygulamaylagerçekleştirilmiştir. 24 ve 72. saatler sonunda numuneleralınmıştır. Ayçiçeği yaprak dokularının (Helianthus annuus L. cv.Tarsan-1018) tüm sinyal moleküllerinin analizi, analiz kitikullanılarak belirlenmiştir. Tuz stresi, sodyum nitroprussid vehormon uygulamaları NO miktarını artırmıştır. IAA ve GA'nınuygulanmasına devam edilmesi, cGMP ve Ca+ 2 miktarında birazalmaya neden olmuştur. Bunların yanı sıra tuz stresi, sodyumnitroprussid ve hormon uygulamaları kontrol grubundan dahayüksek NO, cGMP ve Ca+ 2 miktarları sağlamıştır. Sonuçlar,hormonlar ve sinyal molekülleri arasında sinerjik bir etki olduğunugöstermiştir. Sinyal moleküllerinin stres koşullarına karşıdüzenlendiği gözlenmiştir.
The Effect of Salts Stress, Some Plant Hormones and SNP Applications on Signal Molecules in Helianthus annuus L. Leaves
In this study, the effects of salt stress, sodium nitoprusside and hormones applications on some signal molecules [nitric oxide (NO), cyclic guanosine monofosfate (cGMP) and calcium (Ca+2)] of sunflower (Helianthus annuus L. cv. Tarsan-1018) plant leaf were investigated. Helianthus annuss L. cv. TARSAN – 1018 seeds were obtained through the Edirne Thrace Agricultural Research Institute. Seeds were irrigated with culture solution for 5 weeks. At the end of the fifth week salt, sodium nitoprusside and hormone applications were performed with foliar application for 72 hours. At the end of 24th and 72nd hours samples were taken. All of signal molecules of leaf tissues of sunflower (Helianthus annuus L. cv. Tarsan-1018) were determined by using analysis kit. Salt stress, sodium nitroprusside and hormone applications caused an increase in the level of NO. Continuation of the administration of IAA and GA resulted in a decrease in the amount of cGMP and Ca+2. Besides of these, salt stress, sodium nitroprusside and hormone applications provided higher NO, cGMP and Ca+2 levels than the control group. The results showed that there was a synergistic effect between hormones and signaling molecules. It has been observed that the signal molecules were regulated against stress conditions.
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