AYSEL GÜVEN, Hacı Ahmet DEVECİ, İNAN KAYA, MERYEM DEMİRCAN POYRAZ, Alpaslan Kadir DEVRİM

The investigation of yrecombinant human IGF-I (ınsulin like growth factor-I) ınjection on rats liver malondealdehyde and reduced glutathione levels

Bu çalışmada deney hayvam olarak, ağırlıkları 95±5 g arasında değişen 30 adet dişi rat (Wistar albino), kontrol ve deney olarak 15'er hayvanlık iki gruba ayrıldı ve ayrı kafeslere alındı. Deney grubundaki ratlara 7 gün boyunca her 24 saatte bir 100 ng/kg/gün dozunda rekombinat human Insulin-Like Growth Factor-I (rh IGF-I), kontrol grubuna ise sadece 0.01 M NaHCO3 subkutan olarak enjekte edildi. Yedi günlük enjeksiyonu takip eden 8, 15 ve 22. günlerde ratlar tartılarak karaciğer dokuları alındı. Enjeksiyonun 7. gününde kontrol grubundan alman karaciğer örneklerinde malondialdehit (MDA) ve redükte glutatyon (GSH) düzeylerinde istatistiksel olarak önemli bir değişim görülmedi. Onbeşinci günde MDA düzeylerinde istatistiksel olarak önemli bir artış görülürken, GSH düzeyinde aynı oranda bir azalış görüldü. Yirmiikinci günde hem MDA hem de GSH düzeylerinde önemli bir azalma kaydedildi (p

Anahtar Kelimeler:

glutatyon, insulin benzeri büyüme faktörü, lipit peroksidasyonu, karaciğer, sıçan

Recombinant human IGF-I (Insülin-like growth factor-I)Enjeksiyonu yapılan ratlarda karaciğer malondialdehit ve redükte glutatyon düzeylerinin araştırılması

The effect of insulin-like growth factor-I (IGF-I) administration on lipid peroxidation and reducted glutathione in liver of rats was studied. Total of 30 female rats (Wistar albino), weighing 95±5g, were divided into two groups and kept in two separete cages. The second group (n=15) was rh IGF-I (100 ng/kg/day) in 0.01 M NaHCO3) was subcutananeusly injected tor at for a 7 days period. Also, first group (control group (n=15)) was subcutananeusly received 0.01 M NaCHO3 in same manner with rh IGF-I treated group. At the end of injection period, at 8th, 15th and 22nd days, rats have been weighed and liver tissues were removed. At the 7th injection day, removed liver tissues from control group has been examined and has not been seen any significant changes statistically. At the 15th injection day, has seen that MDA levels are increased (P<0.01) and GSH levels are decreased (P<0.01) statistically. On the other hand at the 22nd day has been recorded that both MDA and GSH levels are decreased. These results showed that IGF-I has been important metabolic effect on the MDA and GSH.

Keywords:

glutathione, insulin-like growth factor, lipid peroxidation, liver, rats,

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