ESİN GÜVEN, HAMZA AVCIOĞLU, İbrahim BALKAYA, ARMAĞAN HAYIRLI, Sirri KAR, Zafer KARAER

Prevalence of cryptosporidiosis and molecular characterization of cryptosporidium spp. in calves in Erzurum

Bu çalışma, Erzurum yöresindeki sütten kesim öncesi dönemdeki buzağılarda cryptosporidiosisin prevalansının ve Cryptosporidium türlerinin moleküler karekterizasyonunun ortaya konması amacıyla yapılmıştır. Bu amaçla beş süt işletmesinden, bir aydan küçük 307 buzağının dışkı örnekleri toplanmış ve DNA ekstraksiyonu (QIAamp DNA Stool kit) yapılarak genomik DNA elde edilmiştir. Nested PCR protokolü ile Cryptosporidium SSU rRNA gen bölgesinin kısmi amplifikasyonu yapılmış ve cryptosporidiosis prevalansı %3.9 olarak belirlenmiştir. Geleneksel yöntemlerle yetiştirilen, dişi, 2 haftalık yaşta ve sulu dışkıya sahip buzağıların modern işletmelerde yetiştirilen, erkek, 3 haftadan büyük ve katı kıvamlı dışkıya sahip olanlara göre hastalıktan daha çok etkilendiği saptanmıştır. PCR pozitif örneklerin SSU rRNA gen bölgesi hedef alınarak yapılan DNA dizi analizleri sonuçlarına göre C. parvum’un hayvanlarda bulunan tek tür olduğu anlaşılmıştır. Sonuç olarak, yörede ekonomik kayıplara neden olan buzağı ishalleri ve ölümlerinde Cryptosporidium türleri ile diğer hastalık etkenlerinin etkileşimlerinin ortaya konması amacıyla daha kapsamlı çalışmaların yapılmasının gerekliliği sonucuna varılmıştır.

Erzurum yöresinde buzağılarda cryptosporidiosisin prevalansı ve cryptosporidium türlerinin moleküler karekterizasyonu

This study was conducted to determine the prevalence of cryptosporidiosis and to identify Cryptosporidium species found in preweaned calves, in Erzurum, Turkey. Fecal samples were collected from 307 calves up to one month old from 5 dairy farms. Genomic DNA was obtained by DNA extraction (QIAamp DNA Stool kit). The prevalence of cryptosporidiosis was determined based on identification through a nested PCR protocol to amplify fragments of the Cryptosporidium SSU rRNA gene. 3.9% of calves were positive for Cryptosporidium. Calves that were subjected to traditional herd management, were female, aged 2 weeks, and had watery feces were affected by the disease at a greater incidence than those were subjected to planned herd surveillance program, were males, were older than 3 weeks, and had firm feces. DNA sequence analysis of the SSU rRNA gene on all of the PCR positive samples ascertained that C. parvum was the only species present. Further studies should be performed comprehensive fecal analysis for other causative agents for association Cryptosporidium species in calf diarrhea and mortality resulting in economic loss in the region.

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