Effects of thymoquinone on plasma leptin, insulin, thyroid hormones and lipid profile in rats fed a fatty diet

Bu çalışmanın amacı standart ve yağlı diyetle beslenen ratlarda Timokinon (TQ)’nun, enerji metabolizmasını düzenleyen plazma hormon seviyeleri ve lipid profiline etkilerini araştırmaktır. Çalışma 5-6 aylık, 200-260 g Wistar Albino rat kullanılarak 6 haftalık sürede 4 deneysel grupla ve her bir grupta 10 rat ile gerçekleştirildi. 1) Kontrol Grubu: Ratlar standart yem ile beslendi ve gastrik gavaj ile 1 ml 0.9% NaCl/kg canlı ağırlık/gün olarak verildi 2) TQ grubu: Ratlar standart yem ile beslendi ve gastrik gavaj ile 1 ml 0.9% NaCl’de çözülmüş 50 mg TQ/kg canlı ağırlık/gün olarak verildi 3) Yağlı Yem (YY) grubu: Ratlar kg’na %50 hayvansal yağ ilavesi yapılmış deneysel diyetle beslendi ve gastrik gavaj ile 1 ml 0.9% NaCl/kg canlı ağırlık/gün verildi 4) YY+TQ grubu: Ratlar kg’na %50 hayvansal yağ ilavesi yapılmış deneysel diyetle beslendi ve gastrik gavaj ile 1 ml 0.9% NaCl’de çözülmüş 50 mg TQ/kg canlı ağırlık/gün olarak verildi. Deneme süresi sonunda kontrol gruba göre TQ grupta plazma insulin, T4, HDL ve LDL seviyeleri azalırken, plazma glikoz seviyesi arttı (P

Yağlı diyet ile beslenen ratlarda timokinonun plazma leptin, insulin, tiroid hormonları ve lipid profiline etkileri

The purpose of this study was to investigate the effects of thymoquinone (TQ) on plasma hormone levels, which regulate energy metabolism, and on the lipid profile of rats fed a standard and fatty diets. The study was performed on four experimental groups for 6 weeks using Wistar Albino rats weighing 200-260 g, aged 5-6 months, with 10 rats in each group. 1) The control group: Rats were fed a standard diet and given 1 ml 0.9% saline /kg body weight/day by gastric gavage. 2) The TQ group: Rats were fed a standard diet and given 50 mg TQ/kg body weight/day dissolved in 1 ml 0.9% saline, by gastric gavage. 3) The fatty diet (FD) group: Rats were fed an experimental diet containing 50% animal fat and given 1 ml 0.9% saline/kg body weight/day by gastric gavage. 4) The FD+TQ group: Rats were fed an experimental diet containing 50% animal fat and given 50 mg TQ/kg body weight/day dissolved in 1 ml 0.9% saline by gastric gavage. At the end of the experimental period, plasma insulin, thyroxine (T4), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels were significantly decreased in the TQ group compared with the control group while the plasma glucose level was increased (P<0.01). In the FD group compared to the control group, HDL and carnitine levels were significantly decreased while plasma leptin, glucose, total triiodothyronine (T3), T4, free triiodothyronine (FT3), free thyroxine (FT4), trigliseride (TG), LDL and very low-density lipoprotein (VLDL) levels were significantly increased. Compared to the FD group, in particular, leptin, T3, FT4, TG, LDL and VLDL levels were significantly decreased in the FD+TQ group except for increased glucose. The study concludes that TQ may be a natural source for the provention of the harmful effects of a fatty diet, due to the dropped levels of leptin, TG, LDL, VLDL and could be considered for use in the field of preventive medicine.

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1. Gilani AH, Jabeen Q, Khan MSAU: A review of medicinal uses and pharmacological activities of Nigella sativa. Pak J Biol Sci, 7, 441-451, 2004.
2. Lautenbacher LM: Schwarzkümmelöl. Dtsch Apoth Ztg, 137, 68-69, 1997.
3. Badary OA, Taha RA, Gamal El-Din AM, Bdel-Wahab MH: Thymoquinone is a potent superoxide anion scavenger. Drug Chem Toxicol, 26, 87-98, 2003.
4. Al-Naqeep G, Ismail M, Yazan LS: Effects of thymoquinone rich fraction and thymoquinone on plasma lipoprotein levels and hepatic low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase genes expression. J Funct Foods, 1, 298-303, 2009.
5. Abu-Abid S, Szold A, Klausner J: Obesity and cancer. J Med, 33, 73-86, 2002.
6. Wouters K, Van Gorp PJ, Bieghs V, Gijbels MJ, Duimel H, Lutjohann D, Kerksiek A, Van Kruchten R, Maeda N, Staels B, Van Bilsen M, Shiri- Sverdlov R, Hofker MH: Dietary cholesterol, rather than liver steatosis, leads to hepatic inflammation in hyperlipidemic mouse models of nonalcoholic steatohepatitis. Hepatology, 48, 474-486, 2008.
7. Özdamar K: SPSS ile Biyoistatistik. 5. Basım, Kaan Kitabevi, Eskişehir, 2003.
8. Zaoui A, Cherrah Y, Alaoui K, Mahassıne N, Amarouch H, Hassar M: Effects of Nigella sativa fixed oil on blood homeostasis in rat. J Ethnopharmacol, 79, 23-26, 2002.
9. Amin KA, Nagy MA: Effect of carnitine and herbal mixture extract on obesity induced by high fat diet in rats. Diabetol Metab Syndr, 1, 17, 2009.
10. Woods SC, D’alessio DA, Tso P, Rushing PA, Cleqq DJ, Benoit SC, Gotoh K, Liu M, Seeley RJ: Consumption of a high-fat diet alters the homeostatic regulation of energy balance. Physilogy Behav, 83, 573-578, 2004.
11. Le PM, Benhaddou-Andaloussi A, Elimadi A, Settaf A, Cherrah Y, Haddad PS: The petroleum ether extract of Nigella sativa exerts lipid- lowering and insulin-sensitizing actions in the rat. J Ethnopharmacol, 94, 251-259, 2004.
12. Considine RV, Sinha MK, Heiman Ml, Kriaugiunas A, Stephens TwW Nyce MR, Ohannesian JP, Marco CC, Mckee LJ, Bauer Tl, Caro JF: Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med, 334, 292-295, 1996.
13. Schwartz MW, Seeley RJ: Neuroendocrine responses to starvation and weight loss. N Engl J Med, 19, 1807-1811,1997.
14. Saladin R, De Vos P, Guerre-Millo M, Leturque A, Girard J, Staels B, Auwerx J: Transient increase in obese gene expression after food intake or insulin administration. Nature, 377, 527-529, 1995.
15. Havel PJ: Peripheral signals conveying metabolic information to the brain: Short-term and long-term regulation of food intake and energy homeostasis. Exp Biol Med, 226, 963-977, 2001.
16. Seeley RJ, Schwartz MW: Neuroendocrine regulation of food intake. Acta Paediatr Suppl, 1999, 428, 58-61, 1999.
17. Yıldız S, Blanche D, Celebi F, Kaya I, Saatçi M, Cenesiz M, Güven B: Effects of short-term high carbohydrate or fat intakes on leptin, growth hormone and luteinizing hormone secretions in prepubertal fat-tailed Tuj lambs. Reprod Dom Anim, 38, 182-186, 2003.
18. Kanter M: Protective effects of thymoquinone on β-cell damage in streptozotocin-induced diabetic rats. TAD, 7, 64-70, 2009.
19. Kanter M, Coşkun Ö, Korkmaz A, Oter Ş: Effects of nigella sativa on oxidative stress and beta-cell damage in streptozotocin-induced diabetic rats. Anat Rec Part A, 279A, 685-691, 2004.
20. Buettner R, Scholmerich J, Bollheimer LC: High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity, 15, 798- 808, 2007.
21. Pari L, Sankaranarayanan C: Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin-nicotinamide induced diabetic rats. Life Sci, 85, 830-834, 2009.
22. Zimmermann-Belsing T, Brabant G, Holst JJ, Feldt-Rasmussen U: Circulating leptin and thyroid dysfunction. Eur J Endocrinol, 149, 257-271, 2003.
23. Kozlowska L, Rosolowska-Huszcz D: Leptin, thyrotropin, and thyroid hormones in obese/overweight women before and after two levels of energy deficit. Endocrine, 24, 147-153, 2004.
24. Brito PD, Ramos CF, Passos MCF, Moura EG: Adaptive changes in thyroid function of female rats fed a high-fat and low-protein diet during gestation and lactation. Braz J Med Biol Res, 39, 809-816, 2006.
25. Avcı G, Küçükkurt I, Küpeli Akkol E, Yesilada E: Effects of escin mixture from the seeds of aesculus hippocastanum on obesity in mice fed on high-fat diet. Pharm Biol, 48, 247-252, 2010.
26. Pelletier P, Gauthier K, Sideleva O, Samarut J, Enrique Silva JE: Mice lacking the thyroid hormone receptor-gene spend more energy in thermogenesis, burn more fat, and are less sensitive to high-fat diet- induced obesity. Endocrinology, 149, 6471-6486, 2008.
27. Thomas-Moya E, Gianotti M, Proenza AM, Llado I: Paraoxonase 1 response to a high-fat diet: Gender differences in the factors involved. Mol Med, 13, 203-209, 2007.
28. Shih DM, Gu L, Hama S, Xia YR, Navab M: Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model. J Clin Invest, 97, 1630-39, 1996.
29. Bell FP, Delucia A, Bryant LR, Patt CS, Greenberg HS: Carnitine metabolism in Macaca arctoides: The effects of dietary change and fasting on serum triglycerides, unesterifled carnitine, esterifled (acyl)carnitine, and b-hydroxybutyrate. Am J Clin Nutr, 36, 15-21, 1982.
30. Ahmad S, Beg ZH: Elucidation of mechanisms of actions of thymoquinone-enriched methanolic and volatile oil extracts from Nigella sativa against cardiovascular risk parameters in experimental hyperlipidemia. Lipids Health Dis, DOI:10.1186/1476-511X-12-86, 2013.
31. Cederblad G: Effect of diet on plasma carnitine levels and urinary carnitine excretion in humans. Am J Clin Nutr, 4, 725-729, 1987.
32. Seccombe DW, James L, Hahn P, Jones E: L-carnitine treatment in the hyperlipidaemic rabbit. Metabolism, 36, 1192-1196, 1987.