Yusuf Kenan DAĞLIOĞLU, CAN ACIPAYAM, Işılay Gökçe BENC, FİLİZ KİBAR, Fatih KÖKSAL

The effectiveness of hesperidin in the prevention of bacterial translocation caused by methotrexate in the Gastrointestinal tract

Metotreksatı (MTX) çocukluk çağı kanserlerinde yaygın olarak kullanılan antimetabolittir. Oksidatif hasardan kaynaklanan gastrointestinal toksisite MTX için önemli bir sınırlayıcı faktördür. MTX’in ince barsak mukozasında morfolojik hasar ve ciddi bariyer fonksiyon bozukluğuna neden olur. Barsak bariyer fonksiyonları bozulduğu zaman, bakteriyel translokasyon görülebilir. Bu çalışmanın amacı, MTX’in neden olduğu bakteriyel translokasyonun önlenmesinde güçlü bir antioksidan olan hesperidinin etkisini değerlendirmektir. Sıçanlara MTX tek doz intraperitoneal yoldan 20 mg/kg/gün verildi. İntestinal bakteriyel translokasyonu önlemek için ardışık 5 gün hesperidin 200 mg/kg/gün gavaj ile uygulandı. Çalışmanın 6. günü ileoçekal bölge, asendan kolon ve mesenterial lenf nodlarından biyopsi örnekleri kültür vasatlarına alındı. MTX grubunda, barsakta daha fazla üreme ve aynı zamanda belirgin bakteriyel translokasyon saptandı (P

Metotreksatın neden olduğu Gastrointestinal kanaldan bakteriyel translokasyonun önlenmesinde hesperidinin etkinliği

Methotrexate (MTX) is an antimetabolite that it is widely used in childhood cancers. Gastrointestinal toxicity stemming from oxidative damage is an important factor limiting its use. MTX causes morphological damage in the mucosa of the small intestine and serious barrier function disorder. Bacterial translocation can be seen when intestinal barrier functions are deteriorated. The aim of this study was to investigate the effect of hesperidin, a powerful antioxidant, in the prevention of bacterial translocation caused by MTX. Rats were given a single intraperitoneal dose of MTX at 20 mg/kg body weight. Hesperidin was given with oral gavage at 200 mg/kg body weight through 5 days. On the 6th day, biopsy specimens from the ileocecal region, ascending colon and mesenteric lymph nodes were placed in culture media. Increased intestinal bacteria growth was found and prominent bacterial translocation were determined in the MTX group (P<0.05). Hesperidin significantly reduced the growth load and bacterial translocation. This study showed that hesperidin protects against translocation by preventing damage caused by MTX.

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