Distribution of different alleles of aromatase cytochrome P450 (CYP19) and melatonin receptor 1A (MTRN1A) genes among native Turkish sheep breeds

Bu çalışmada Türkiye yerli koyun ırklarından (Akkaraman, Dağlıç, Gökçeada, İvesi, Karacabey Merinosu, Karayaka, Kıvırcık, Sakız) 257 hayvan üreme özellikleri açısından önem taşıyan CYP19 ve MTRN1A genlerinde bulunan üç adet TNP bakımdan incelenmiştir. CYP19 lokusunda bulunan A→G transisyonu ile MTRN1A lokusunda bulunan C→T ve G→A transisyonları PCR-RFLP metodu kullanılarak incelenmiştir. CYP19 lokusunu analiz etmek için DraI, MTRN1A lokusunu analiz etmek için MnlI ve RsaI restriksiyon enzimleri kullanılmıştır. İncelenen tüm lokuslar için iki allel bulunmuştur. Bu alleler CYP19 lokusu için A ve G MTRN1A-MnlI lokusu için M ve m, MTRN1A-RsaI lokusu için R ve r olarak isimlendirilmiştir. CYP19 ve MTRN1A-MnlI lokusları için sırasıyla A ve m alleleri predominanttır. MTRN1A-RsaI lokusu bakımdan incelenen ırkların çoğunda R alleli yaygın iken, Sakız, Karacabey Merinosu ve Karayaka ırklarında r alleli predominanttır. UPGMA test sonuçları bu lokusların allel frekans dağılımlarının ırklar arasındaki genetik mesafenin hesaplanmasında kullanılabileceğini göstermiştir.

Aromatase cytochrome P450 (CYP19) ve melatonin receptor 1A (MTRN1A) genlerinin farklı allelerinin Türkiye yerli koyun Irkları arasındaki dağılımı

In this study, 257 animals from eight native Turkish sheep breeds (Akkaraman, Dağlıç, Gökçeada, İvesi, Karacabey Merino, Karayaka, Kıvırcık, Sakız) were investigated for three SNPs located at CYP19 and MTRN1A genes which are important for reproductive traits. The A→G transition within CYP19, the C→T and G→A transitions within MTRN1A were investigated by using PCR-RFLP method. To analyze CYP19 locus DraI, for MTRN1A locus MnlI and RsaI restriction enzymes were used. Two alleles were found for all the loci investigated. These alleles named as A and G for CYP19, M and m for MTRN1A-MnlI, and R and r for MTRN1A-RsaI. A and m alleles were predominant at CYP19 and MTRN1A-MnlI loci, respectively. While R allel was prevalent in majority of breeds investigated, r allele was predominant in Sakız, Karacabey Merino, and Karayaka breeds for MTRN1A-RsaI. UPGMA test results show that the allele frequencies distributions of these loci can be used for calculating genetic distance between breeds.

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