DİDEM PEKMEZCİ, Aziz Arda SANCAK, Orhan PINAR, Duygu ÇAKIROĞLU, SERHAT ARSLAN

Allergen determination in thoroughbred stallions via detecting serum specific IgE

Atlarda alerjik hastalıklar atopik dermatitis, tekrarlayan ürtiker ve tekrarlayan havayolu hastalıklarının patofizyolojisinde rol oynamaktadır. Bu çalışma ile İngiliz aygırlarında serum spesifik IgE tespiti ile alerjenlerin tayini hakkında bilgi verilmesi amaçlanmıştır. Yaşları ortalama 11 olan, 18 İngiliz aygır çalışma materyalini oluşturmuştur. On beş tek ve beş karışık alerjene serum spesifik IgE yönünden bakılmıştır. Toplam altı İngiliz aygırda English plantain (Plantago lanceolata), Birch/Alder/Hazel ve Micropolyspora faeni/Thermoactinomyces oldukça alerjik olarak tespit edilmiştir. Sonuç olarak, atlara özgü IgE’yi belirleyen serolojik testler alerjenlerin tespiti bakımından zahmetsiz bir yöntem olarak değerlendirilebilir.

İngiliz aygırlarında serum spesifik IgE tespiti ile alerjen tayini

In horses, allergic disease has been implicated in the pathophysiology of atopic dermatitis, recurrent urticaria, and recurrent airway obstruction. With the present study, it is aimed to give information about allergens which determined in Thoroughbred stallions with detecting serum IgE. 18 Thoroughbred stallions averaged 11 years old were included in the study. Fifteen single and five mix allergens were checked for allergen-specific IgE. English plantain (Plantago lanceolata), Birch/Alder/Hazel and Micropolyspora faeni/Thermoactinomyces were detected as quite allergic to six Thoroughbred stallions at total. Consequently, assays detect equine IgE, can be an effortless method in determining allergens in horses.

PDF

___

1. Lorch G, Hillier A, Kwochka KW, Saville WA, Kohn CW, Leroy BE: Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergo sorbent test and two ELISA in horses with and without atopy. JAVMA, 218, 1314-1322, 2001.
2. Kalina WV, Pettigrew HD, Gershwin LJ: IgE ELISA using antisera derived from epsilon chain antigenic peptides detects allergen-specific IgE in allergic horses. Vet Immunol Immunopathol, 92, 137-147, 2003.
3. Wagner B, Miller WH, Morgan EE, Hillegas JM, Erb HN, Leibold W, Antzakc DF: IgE and IgG antibodies in skin allergy of the horse. Vet Res, 37, 813-825, 2006.
4. Wilson AD, Harwood LJ, Björnsdottir S, Marti E, Day MJ: Detection of IgG and IgE serum antibodies to Culicoides salivary gland antigens in horses with insect dermal hypersensitivity (sweet itch). Equine Vet J, 33, 707-713, 2001.
5. White DS: Advances in equine atopic dermatitis, serologic and intradermal allergy testing. Clin Tech Equine Pract, 4, 311-313, 2005.
6. Famoye F, Singh K: Zero-inflated generalized poisson regression model with an application to domestic violence data. J. Data Sci, 4, 117- 130, 2006.
7. Lambert D: Zero-inflated poisson regression models with an application to defects in manufacturing. Technometrics, 34, 1-14, 1992.
8. Rendle DI, Durham AE, Wylie CE, Newton JR: Results of intradermal testing for the investigation of atopic dermatitis and recurrent urticaria in 50 horses in the south of England. Equine Vet Educ, 22 (12): 616-622, 2010.
9. Scott DW, Miller WH: Skin immune system and allergic skin diseases. In, Scott DW, Miller WH (Eds): Equine Dermatology. 395-374, St Louis: WB Saunders, 2003.
10. Negrini AC, Arobba D: Allergenic pollens and pollinosis in Italy: Recent advances. Allergy, 47, 371-379, 1992.
11. Robinson NE, Derksen FJ, Olszewski M, Buechner-Maxwell V: The pathogenesis of chronic obstructive pulmonary disease. Br Vet J, 152, 283- 306, 1996.
12. Halliwell RE, Mcgorum BC, Irving P, Dixon PM: Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease. Vet Immunol Immunopathol, 38, 201-215, 1993.
13. Littlewood JD: Incidence of recurrent seasonal pruritus (“sweet itch”) in British and German shire horses. Vet Res, 142, 66-67, 1998.