BUKET BAKIR, Ebru KARADAG SAR, Sevda ELİŞ YILDIZ, HASAN ASKER

Effects of Thymoquinone Supplementation on Somatostatin Secretion in Pancreas Tissue of Rats

Bu çalışmada, Nigella sativa (Çörek otu) çekirdeğinin biyoaktif fitokimyasal bileşeni olan timokinonun (TQ), rat pankreas dokusundaki somatostatin salgısı üzerine etkisinin, immünohistokimyasal yöntem kullanarak incelenmesi amaçlandı. Hayvanlar (n=30) kontrol, sham ve timokinon olmak üzere üç gruba ayrıldı. TQ grubuna, 8 mg/kg TQ 14 gün boyunca enjekte edilirken sham grubuna aynı süre sadece serum fizyolojik enjekte edildi. Kontrol grubuna ise herhangi bir uygulama yapılmadı. Histolojik incelemeler için doku kesitlerine Crosman'ın üçlü boyama yöntemi uygulandı. Pankreas dokusunda somatostatin immunoreaktivitesini incelemek için Streptavidin-Biotin-Peroxidase Complex metodu kullanıldı. Spesifik somatostatin immünoreaktivitesi tüm grupların endokrin hücrelerinde (langerhans adacıkları) tespit edildi. Somatostatin sekresyonunun kontrol ve sham grupları ile karşılaştırıldığında thymoquinone grubunda arttığı tespit edildi. Sonuç olarak, TQ uygulamasının, büyüme hormonu, insulin, glukagon, sekretin, ve gastrin gibi hormonların salınımını düzenlediği bilinen somatostatinin salgısını arttırdığı sonucuna varıldı

Timokinon Uygulamasının Ratların Pankreas Dokusunda Somatostatin Salgısı Üzerine Etkileri

In this study, it was aimed to investigate the effects of thymoquinone (TQ), which is the bioactive phytochemical constituent of the seeds oil of Nigella sativa, on somatostatin secretion in the rat pancreatic tissue by immunohistochemical method. Animals (n=30) were divided into 3 groups as follows: control, sham and thymoquinone. While Thymoquinone group received 8 mg/kg of TQ which injected for 14 days, only isotonic saline was injected to the sham group for same time. The control group received nothing. Crossman's triple staining was applied to tissue sections to examine histology. Streptavidin-Biotin-Peroxidase Complex method was used to investigate somatostatin immunoreactivity in the pancreatic tissue. Specific somatostatin immunoreactivities were observed in endocrine cells (islets of Langerhans) in all groups. It was determined that somatostatin secretion increased in the thymoquinone group compared to control and sham. In conclusion, thymoquinone administration was concluded to increase the secretion of somatostatin that is known to regulate certain hormones such as growth hormone, insulin, glucagon, secretin and gastrin

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