Hamid Reza SEYEDABADI, Sima SAVAR SOFLA

Microsatellite Analysis for Parentage Verification and Genetic Characterization of the Turkmen Horse Population

Mikrosatellitler yaygın olarak soy tespiti amacıyla ve popülasyon çalışmalarında kullanılan bir sınıf genetik belirteçlerdir. Bu çalışma, Türkmen at popülasyonunda identifikasyon ve soy analizinde mikrosatellit belirteçlerin kullanılabilirliğini tespit etmek amacıyla yapılmıştır. Bu amaçla, 574 ergin (92 aygır, 345 beygir ve 137 kısrak) ve 174 tay (98 erkek tay ve 76 dişi tay) içeren toplam 748 Türkmen ata ait örnekler ISAG tarafından önerilen 17 mikrosatellit belirteç kullanılarak genotiplendirildi. Her bir lokusta allel sayısı 5 (HMS01 ve HTG07) ile 10 (HTG10) arasında olmak üzere ortalama 7.65 olarak tespit edildi. Gözlemlenen heterozigotluk ve beklenen heterozigotluk sırasıyla 0.365-0.953 (ortalama 0.703) ve 0.617-0.884 (ortalama 0.792) olarak belirlendi. Polimorfizm bilgi içeriği ortalama 0.767 olmak üzere 0.586 (HTG7) ile 0.873 (HTG10) arasında değişim gösterdi. 17 mikrosatellit bölgenin total dışlama olasılığı 0.9999 idi. Mikrosatellit belirteçler kullanılarak yapılan Türkmen atlarındaki soy araştırması soy kayıtlarındaki hataları tespit etmede etkiliydi. DNA tiplendirme metodu soy kayıtlarının sistemik kontrolünde yüksek potansiyele sahip olup Türkmen atlarının genetik kaynağını artırmada kullanılabilir

Türkmen At Popülasyonunda Soy Tespiti amacıyla Mikrosatellit Analiz ve Genetik Karakterizasyon

Microsatellites are a class of genetic markers commonly used for parentage verification and population studies. This study determined the efficiency of microsatellite markers for identification and pedigree analysis in horses based on the example of Turkmen horse population. For this purpose, 748 Turkmen horse samples including 574 adults (92 stallions, 345 males and 137 mares) and 174 foals (98 colts and 76 fillies),were genotyped by using seventeen microsatellite markers recommended by ISAG. The number of allele per locus varied from 5 (HMS01 and HTG07) to 10 (HTG10) with an average value of 7.65. The observed heterozygosity and the expected heterozygosity ranged 0.365-0.953 (mean 0.703), from 0.617-0.884 (mean 0.792) respectively. PIC value ranged from 0.586 (HTG7) to 0.873 (HTG10) with average 0.767. The total exclusion probability of the 17 microsatellite loci was 0.9999. The pedigree study of the Turkmen horse using microsatellite markers was efficient in detecting mistakes during genealogical records. These results suggested that the DNA typing method had high potential for systematic control of the genealogical registrations and genetic resources to improve genetic aspects in Turkmen horses

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