Gene Primer SEQUENCE, H. Kaan MÜŞTAK, Kaan ÖNAT, K. Serdar DIKER

Evaluation of Virulence Factors and Phylogrouping of Escherichia coli Strains Isolated from Acute Bovine Mastitis in Turkey

Çalışmanın amacı akut sığır mastitinden izole edilen 155 E. coli izolatında, yaygın olarak çalışılan virulens faktörlerinin filogenetik dağılımını belirlemekti. İzolatların filogruplandırması yapıldığında, A, B1, B2, C, D, E, F grupları ve Sınıf I tespit edildi. Kommensal suşları oluşturan, B1 ve C sırasıyla, %45.2 ve %37.4 oranla en yaygın filogruplar olarak bulundu. Bu çalışmada, diğer filogruplar, %0.6-%8.9 aralığındaki oranda F, D, A, and E olarak tespit edildi. Virulens faktörlerini belirlemek için en çok bilinen virulens genleri; clpg, F17, afa, iucC, iucD, cnf1, cnf2, kps ve traT seçildi. traT, suşların büyük çoğunluğunda (%66.4) tespit edilen virulens genidir. Filogruplar ve virulens faktörleri arasında herhangi bir ilişki belirlenmedi. Akut sığır mastitis olgularında, C, E. F filogrupları ilk kez tespit edildi

Akut Sığır Mastitislerinden İzole Edilen Escherichia coli Suslarının Filogruplandırılması ve Virulens Faktör Değerlendirmesi

The objective of this study was to determine the phylogenetic distribution of commonly studied virulence factors of 155 E. coli isolated from acute bovine mastitis. In the study, A, B1, B2, C, D, E, F phylogroups, and Clade I were defined. B1 and C were found to be the most prevalent phylogroups covering commensal strains with a ratio of 45.2% and 37.4%, respectively. The other phylogroups determined in the study were F, D, A, and E with a range of 0.6%-8.9%. Commonly known virulence genes; clpg, F17, afa, iucC, iucD, cnf1, cnf2, kps, and traT were selected to determine the virulence factors. The only virulence factor gene existing within the strains was traT (66.4%). However, any relation between phylogroups and virulence factors was not determined. Phylogroups C, E and F were found to be new phylogroups for the first time in acute bovine mastitis cases

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