Ayşe EKEN, Orhan YÜCEL, İFFET İPEK BOŞGELMEZ, AYŞE BALDEMİR KILIÇ, Sezai ÇUBUK, Ali Hakan ÇERMİK, Burcu ENDİRLİK ÜNLÜ, Elçin BAKIR, Akın YILDIZHAN, Adem GÜLER, MÜBERRA KOŞAR

An Investigation on Protective Effect of Viburnum opulus L. Fruit Extract Against Ischemia/Reperfusion-Induced Oxidative Stress After Lung Transplantation in Rats

Bu çalışma, akciğer transplantasyonu sırasında iskemi/reperfüzyonun indüklediği oksidatif hasara karşı Viburnum opulus L. meyve ekstresinin koruyucu etkisini araştırmak amacıyla yapılmıştır. Bu amaçla, ilk olarak donör ve alıcı oluşturmak için 30 dişi rat rastgele seçildi. Daha sonra, ratlar kontrol, I/R ve V. opulus + I/R olarak adlandırılan üç gruba ayrıldı. Deneysel ratlara iskeminin ardından 2 saat reperfüzyon ile akciğer transplantasyonu yapıldı. Transplantasyon ile ilişkili akciğer hasarı, farklı parametrelerle incelendi. Kontrol grubu ile karşılaştırıldığında I/R grubunun akciğer doku örneklerinde süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve katalaz (CAT) enzim aktiviteleri, glutatyon ve total antioksidan durum (TAS) düzeylerinde önemli bir azalış, malondialdehit (MDA), total oksidan durum (TOS) ve protein karbonil düzeylerinde anlamlı artış belirlendi. Bununla birlikte, V. opulus meyve ekstresi ile uygulama sonrası MDA ve protein karbonil düzeylerinde önemli azalma ve antioksidan sistemde artma saptanmıştır. Sonuç olarak, akciğer transplantasyonu sırasında I/R'nin indüklediği oksidatif strese karşı V. opulus meyve ekstresinin muhtemel radikal temizleme ve antioksidan aktivitesi ile koruyucu etkilerinin olduğunu göstermiştir. Bu nedenle, bu meyve ekstresi I/R ilişkili akciğer hasarının önlenmesinde etkili olabilir

Ratlarda Akciğer Transplantasyonda Iskemi/Reperfüzyonun İndüklediği Oksidatif Strese Karşı Viburnum opulus Meyve Ekstresinin Koruyucu Etkisinin Araştırılması

The aim of this study was to investigate the protective effect of Viburnum opulus L. fruit extract against ischemia/reperfusion (I/R)-induced oxidative stress during the lung transplantation. For this purpose, 30 female rats were firstly randomized to form of donor and recipients. After then, the rats were divided into three groups named as control, I/R, and V. opulus + I/R. Experimental rats were subjected to lung transplantation with ischemia followed by 2 h of reperfusion. Transplantation-related lung injury was evaluated by multiple parameters. A significant decrease was observed in the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), and the levels of glutathione and total antioxidant status (TAS), whereas the levels of malondialdehyde (MDA), total oxidant status (TOS), and protein carbonyl were significantly increased in lung tissue samples of I/R group in comparison to the control group. However, treatment with V. opulus fruit extract resulted in significant reduction of MDA and protein carbonyl levels and increment of the antioxidant system. In conclusion, V. opulus fruit extract showed protective effects against I/R-induced oxidative stress during lung transplantation probably by the radical scavenging and antioxidant activity. Therefore, this fruit extract can be efficient in the prevention of I/R-related lung toxicity

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