Wenyan GAI, Junwei WANG, Juan WANG, Zhigang CUI, Zhina QU, Yundong WANG, Jun HONG, Jinghua CUI, Shigan YAN, Xiaoli DU, Xiumei HUANG, Jianmei ZHAO, Liping ZHU

Molecular Typing and Drug Resistance Analysis of Salmonella spp. Isolated from Pig Slaughterhouse in Shandong Province, China

Çin'in Shandong Eyaletindeki sekiz kesimhaneden Aralık 2014 ile Ekim 2015 tarihleri arasında alınan domuz örneklerinde Salmonella enteritisi için epidemiyolojik bir çalışma yürütüldü. Kesimhane örneklerinin %22.2'sinden (142/640) Salmonella spp. tespit edildi. Tüm Salmonella pozitif örnekler serotiplendirme, antimikrobiyal dirençlilik testi, değişken alanlı jel elektroforez (PFGE) ve multilokus sekans tiplendirmesi (MLST) ile karakterize edildi. En yaygını Salmonella Derby, Salmonella Typhimurium ve Salmonella Thompson olmak üzere toplam on serotip tüm izolatlarda gözlemlendi. Antimikrobiyal hassaslık testi birçok çoklu ilaç direnciyle (MDR) birlikte en yüksek antimikrobiyal direncin sulfisoxazole (%91.55) karşı olduğunu gösterdi. MLST analizi dokuz sekans tipi (ST) şeklinin olduğunu gösterdi. Bu sekans tiplerinden en yaygını ST40 (79 izolat) daha az olarak ise ST19 (26 izolat) ve ST26 (24 izolat) olarak belirlendi. PFGE yüksek benzerlik göstererek tüm izolatların Xbal makrorestriksiyon parçalarının rezolüsyonunu sağladı. Üç küme ve 31 PFGE şekli PFGE analizi ile üretildi. Elde edilen sonuçlar, sekiz kesimhaneden izole edilen Salmonella spp.'nin fenotipik ve genotipik olarak homolog olduğunu gösterdi. Bu bulgular ileriki analizler için kullanılabilir

Çin'in Shandong Eyaletinde Domuz Mezbahasından İzole Edilen Salmonella spp.'nin Moleküler Tiplendirilmesi ve İlaç Dirençliliği Analizi

An epidemiological investigation of Salmonella enteritidis in pig and pork samples from eight slaughterhouses in Shandong Province, China, was conducted from December 2014 to October 2015. A total of 22.2% (142/640), of the slaughterhouse samples were recovered positive for Salmonella spp.. All Salmonella-positive were characterized using serotyping, antimicrobial resistance testing, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Ten serotypes were shared by all isolates, with the most common serotypes being Salmonella Derby, Salmonella Typhimurium, and Salmonella Thompson. Antimicrobial sensitivity testing revealed that the highest antimicrobial resistance rate was against sulfisoxazole (91.55%) with many multidrug-resistant (MDR) isolates. MLST analysis showed that nine sequence type (ST) patterns were shared, ST40 was the most common (79 isolates) followed by ST19 (26 isolates) and ST26 (24 isolates). PFGE permitted the resolution of XbaI macrorestriction fragments of all the isolates, displaying the high similarity. Three clusters and 31 PFGE patterns were generated by PFGE analysis. Our results indicated that Salmonella spp. isolates from eight slaughterhouses were phenotypically and genetically homologous. These data could be used for further evolutionary analyses

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