Huong Thi Thanh DOAN, Xuyen Thi Kim LE, Roan Thi DO, Khue Thi NGUYEN, Thanh Hoa LE

223222

Sequencing and Phylogenetic Analysis Reveal the Prevalence of Duck Hepatitis A Virus Genotype-3 in Vietnam

Ördek Hepatitis A virüsü (DHAV) dünya çapında ördek palazlarında akut ve oldukça bulaşıcı bir hastalığa neden olmaktadır. DHAV aşısının yaygın kullanımına rağmen Vietnam'da hala birçok salgınlar oluşmaktadır. Bu çalışmada, 2009 ve 2013 yıllarında enfekte ördeklerden elde edilen iki adet DAHV izolatının (NC ve NT) tüm genom sekansı belirlenmiştir ve Vietnam'da mevcut kullanılan atenüe DHAV-1 aşı suşu (VXXT) ile karşılaştırılmıştır. NC ve NT suşları virulent DHAV-3 genotipine ait olup genomları sırasıyla 7791 ve 7790 nükleotid (nt)'ten oluşmaktadır. Her iki genom 6756 nt boyutunda bir büyük açık okuma çerçevesine (open reading frame ORF), sahip olup 2251 amino asit uzunluğunda bir poliprotein kodlar ve a tipik olarak picornavirus genom organizasyona sahiptir. Poliproteinde tahmini C-terminal ayrılma bölgelerinin çoğunluğu Q/S veya Q/G olarak belirlendi. Bütün ORF'nin nükleotid sekansının filogenetik analazi tüm virülent Vietnam suşlarının Çin DHAV-3 suşu ile yakından akraba olduğunu gösterdi. Virülent Vietnam DHAV-3 suşları göreceli olarak DHAV-1 aşı suşu ile düşük nükleotid benzerliğine (%73.5) sahipti. Bu çalışma antijen uyumlu bir DHAV-3 aşısının Vietnam'da acilen kullanılması gerekliliğini göstermektedir

Sekans ve Filogenetik Analiz Vietnamda Ördek Hepatitis A Virüs Genotip-3'ün Prevalansını Ortaya Koymaktadır

Duck hepatitis A virus (DHAV) causes an acute and highly contagious disease in young ducklings worldwide. Despite the widespread use of the DHAV vaccine, many outbreaks still occur in Vietnam. In this study, we determined the full-length genome sequence of two DHAV isolates (NC and NT) obtained from infected ducks in 2009 and 2013, and compared them with the attenuated DHAV-1 vaccine strain (namely, VXXT) currently used in Vietnam. The NC and NT strains belong to the virulent DHAV-3 genotype, and their genomes consist of 7791 and 7790 nucleotides (nt), respectively. Both genomes contain one large open reading frame (ORF) of 6756 nt, encoding a polyprotein of 2251 amino acids and possess a typical picornavirus genome organization. The majority of predicted C-terminal cleavage sites in the polyprotein were Q/S or Q/G. Phylogenetic analysis of the nucleotide sequence of the full ORF revealed that all virulent Vietnamese strains are closely related to Chinese DHAV-3 strains. Noticeably, the virulent Vietnamese DHAV-3 strains had relatively low nucleotide identity with the DHAV-1 vaccine strain (73.5%). The study showed that an antigenicity-matched DHAV-3 vaccine is urgently required for use in Vietnam
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Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: 6
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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