Hacı Ahmet DEVECİ, ABDULSAMED KÜKÜRT, ERDOĞAN UZLU, İBRAHİM SÖZDUTMAZ, OĞUZ MERHAN, MUSTAFA SİNAN AKTAŞ, MERVE ALPAY, İNAN KAYA, MAHMUT KARAPEHLİVAN

Evaluation of Paraoxonase Activity, Total Sialic Acid and Oxidative Stress in Sheep with Ecthyma Contagiosa

Zoonozlar, dünya sağlık organizasyonu tarafından omurgalı hayvanlar ve insanlar arasında doğal olarak iletilen hastalıklar ve enfeksiyonlar olarak tanımlanmaktadır. Pox virüslü hayvanların oluşturduğu zoonotik riskin saptaması ve bertaraf edilmesi hem insan hem de hayvan sağlığı için önemlidir. Bulaşıcı ektima koyun, keçi, diğer bazı evcil ve vahşi hayvanları etkileyen bulaşıcı, zoonotik, viral cilt hastalığıdır. Sunulan bu çalışmada sağlıklı ve ektima ile doğal enfekte koyunlarda plazma paraoksonaz aktivitesi (PON1), yüksek dansiteli lipoprotein (HDL), toplam sialik asit (TSA), malondialdehit (MDA), nitrik oksit (NO) ve toplam kan glutatyonu seviyeleri tespit edildi ve sonuçları değerlendirildi. Sağlıklı koyunlarda PON1 218.54±17.93 U/L, TSA 59.89±5.59 mg/dL, HDL 48.4±4.88 mg/dL, MDA 8.58±0.80 µmol/L, NO 7.78±1.02 µmol/L ve GSH 21.11±3.70 mg/dL olarak belirlendi. Ektima ile enfekte koyunlarda ise bu değerler sırasıyla 174.92±18.68 U/L, 70.1±6.56 mg/dL, 37.9±6.47 mg/dL, 11.26±1.06 µmol/L, 12.44±1.90 µmol/L, 7.79±0.90 mg/dL tespit edildi. Sonuç olarak, ektima ile enfekte koyunlarda, pro-oksidan ve antioksidan moleküller arasındaki dengesizliğe bağlı oksidatif stres oluştuğu, bu dengenin artan oksidan seviyelerine bağlı olduğu saptandı

Ecthyma Contagiosa'lı Koyunlarda Paraoksonaz Aktivitesi, Total Sialik Asit ve Oksidatif Stresin Değerlendirilmesi

Zoonoses are defined by the world health organization as diseases and infections which are transmitted naturally between vertebrate animals and human. Undestanding the zoonotic risk posed by pox viruses in companion animals is important for both human and animal health. Contagious ecthyma is highly contagious, zoonotic, viral skin disease that affects sheep, goats and some other domesticated and wild animals. In this present study was detected and evaluated levels of plasma paraoxonase activity (PON1), high-density lipoprotein (HDL), total sialic acid (TSA), malondialdehyde (MDA), nitric oxide (NO) and total blood glutathione (GSH) concentrations in healthy sheep and natural infected sheeps with ecthyma. In healthy sheep, laboratory results were determined as PON1 218.54±17.93 U/L, TSA 59.89±5.59 mg/dL, HDL 48.4±4.88 mg/dL, MDA 8.58±0.80 ?mol/L, NO 7.78±1.02 ?mol/L and GSH 21.11±3.70 mg/dL. These values were found 174.92±18.68 U/L, 70.1±6.56 mg/dL, 37.9±6.47 mg/dL, 11.26±1.06 µmol/L, 12.44±1.90 µmol/L, 7.79±0.90 mg/dL respectively in sheeps wich are infected by ecthyma. As a result, it was concluded that there is oxidative stress due to imbalance between pro-oxidant and antioxidant molecules in sheep which are infected by ecthyma, and this imbalance is shaped by increasing oxidant levels

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