BELMA ASLIM, Dilşad ONBAŞILI, Zehra Nur YÜKSEKDAĞ

Determination of lactic acid production and antagonistic activity against Helicobacter pylori of L. delbrueckii subsp. bulgaricus and S. thermophilus strains

Bu çalışmada, toplam 32 adet lactobacilli and streptococci suşlarının laktik asit üretimleri belirlenmiştir. Laktik asitin farklı miktarları, çalışmadaki suşlar tarafından üretilmiştir, fakat laktik asit düzeyleri Lactobacillus delbrueckii subsp. bulgaricus suşları için 13.4-18.6 mg/ mL, Streptococcus thermophilus suşları için 0.6-11.5 mg/mL arasındadır. Antimikrobiyal aktivite, Helicobacter pylori ATCC 43504’ ye karşı belirlenmiştir. Bazı suşlar H. pylori’yi inhibe etmiştir. Baktriyosin benzeri maddeler üreten suşlar organik asitlerin etkilerini ortadan kaldıran koşullar altında belirlenmiştir. Sonuç olarak sadece on suşun inhibitor aktivitesinin bakteriyosin benzeri maddelerden dolayı olduğu görülmüştür.

Anahtar Kelimeler:

Streptococcus thermophilus, agonistler, Helicobacter pylori, laktik asit, Lactobacillus delbrueckii subsp. bulgaricus

L. delbrueckii subsp. bulgaricus ve S. thermophilus suşlarının Helicobacter pylori’ye karşı antogonistik aktivitesi ve laktik asit üretimlerinin belirlenmesi

In this study, lactic acid production was determined of a total of 32 strains of lactobacilli and streptococci. Different amounts of lactic acid were produced by strains studies; however, lactic acid levels were 13.4 to 18.6 mg/mL for Lactobacillus delbrueckii subsp. bulgaricus strains and 0.6 to 11.5 mg/mL for Streptococcus thermophilus strains. Antimicrobial activity was tested against Helicobacter pylori ATCC 43504. The some strains were able to inhibit H. pylori. Bacteriocin-like substances (BLS) producing strains were determined under conditions eliminating the effects of organic acids. As a result, it was observed that inhibitory activities of only ten strains were due to bacteriocin-like substances.

Keywords:

Streptococcus thermophilus, agonists, Helicobacter pylori, lactic acid, Lactobacillus delbrueckii subsp. bulgaricus,

PDF

___

1. Huang HY, Huang SY, Chen PY, King VAE, Lin YP, Tsen JH: Basic Characteristics of Sporolactobacillus inulinus BCRC 14647 for potential probiotic properties. Curr Microb, 54, 396-404, 2007.
2. Aslim B, Beyatli Y, Yuksekdag ZN: Productions and monomer compositions of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains isolated from traditional home-made yoghurts and raw milk. Int J Food Sci Tech, 41, 973-979, 2006.
3. Kim P, Young JM, Young-Hyo C, Saehun K, Seong-Jae K, Yong-Ha P: Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. Appl Microb Biotechnol, 74, 1103-1111, 2007.
4. Panesar PS, Kennedy JF, Knill CJ, Kosseva MR: Applicability of pectate-entrapped Lactobacillus casei cells for L(+) lactic acid production from whey. Appl Microb Biotechnol, 74, 35-42, 2007.
5. Rojo-Bezares B, Sáenz Y, Zarazaga M, Torres C, Ruiz-Larrea F: Antimicrobial activity of nisin against Oenococcus oeni and other wine bacteria. Int J Food Microb, 116, 32-36, 2007.
6. Juven BJ, Schived F, Lindner P: Antagonistic compounds produced by a chicken intestinal strain of Lactobacillus acidophilus. J Food Protec, 55, 157-161, 1992.
7. Yuksekdag ZN, Beyatli Y, Aslim B: Metabolic activities of Lactobacillus spp. strains isolated from kefir. Nahrung/Food, 48, 218-220, 2004.
8. Aslim B, Yuksekdag ZN, Sarikaya E, Beyatli Y: Determination of the bacteriocin-like substances produced by some lactic acid bacteria isolated from Turkish dairy products. LWT/Food Sci Tech, 38, 691-694, 2005.
9. Weber H: Dry sausage manufacture, the importance of protective cultures and their metabolic products. Fleischwirtschaft, 74, 278-282, 1994.
10. Tsai CC, Huang LF, Lin CC, Tsen HY: Antagonistic activity against Helicobacter pylori infection in vitro by a strain of Enterococcus faecium TM39. Int J Food Microb, 96, 1-12, 2004.
11. Lorca GL, Wadström T, De Valdez GF, Ljungh A: Lactobacillus acidophilus autolysins inhibit Helicobacter pylori in vitro. Curr Microb, 42, 39-44, 2001.
12. Hamilton-Miller JMT: The role of probiotics in the treatment and prevention of Helicobacter pylori infection. Int J Antimicrob Agents, 22, 360-366, 2003.
13. Takahashi T, Matsumoto T, Nakamura M, Matsui H, Tsuchimoto K, Yamada H: L-Lactic acid secreted from gastric mucosal cells enhances growth of Helicobacter pylori. Helicobacter, 12, 532-540, 2007.
14. Tunail N, Ayhan K, Akçelik M, Durlu-Özkaya F, Doğan HB, Tükel Ç, Kaleli D, Acar E: Yoğurt fabrikalarında faj probleminin çözümüne yönelik araştırmalar. TÜBİTAK PROJE. NO: TARP-2106, 162s, 2002.
15. Tilsala-Timisjarvi A, Alatossava T: Enzymatic amplification of 16s rRNA gene sequences from dairy Lactobacillus species. Milchwissenschaft, 48, 123-125, 1993.
16. Ventura M, Casas IA, Morelli L, Callegari M: Rapid amplified ribosomal DNA restriction analysis (ARDRA) identification of Lactobacillus spp. isolated from fecal and vaginal samples. Syst Appl Microb, 23, 504- 509, 2000.
17. Roy D, Sirois S, Vincent D: Molecular discrimination of Lactobacilli used as starter and probiotic cultures by amplified ribosomal DNA restriction analysis. Curr Microb, 42, 282-289, 2001.
18. Steinholt K, Calbert HE: A rapid colorimetric method for the determination of lactic acid in milk and milk products. Milchwissenschaft, 15, 7-11, 1960.
19. Reinheimer JA, Demkow MR, Condioti MC: Inhibition of coliform bacteria by lactic cultures. Aust J Dairy Tech, May, 5-9, 1990.
20. Attaie R, Whalen PJ, Shahani KM, Amer, MA: Inhibition of growth of Staphylococcus aureus during production of acidophilus yoghurt. J Food Protect, 50, 224-228, 1987.
21. Okereke A, Montville TJ: Bacteriocin inhibition of Clostridium botulinum spores by lactic acid bacteria. J Food Protect, 54, 349-353.
22. Tagg JR, Dajani AS,Wannamaker LW: Bacteriocins of Gram- positive bacteria. Bacteriol Rev, 40, 722-756, 1976.
23. Zhu WM, Liu W, Wu DQI: Isolation and characterization of a new bacteriocin from Lactobacillus gasseri KT7. J Appl Microb, 88, 877-886, 2000.
24. Temmermanan R, Pot B, Huys G, Swings J: Identification and antibiotic susceptibility of bacterial isolates from probiotic products. Int J Food Microbiol 81, 1-10, 2003.
25. Boonmee M, Leksawasdi N, Bridge W, Rogers PL: Batch and continuous culture of Lactococcus lactis NZ133: experimental data and model development. Biochem Eng J, 14, 127-135, 2003.
26. Gursoy A, Durlu-Özkaya F, Yıldız F, Aslim B: Set type yoghurt production by exopolysaccharide producing Turkish origin domestic strains of Streptococcus thermophilus (W22) and Lactobacillus delbrueckii ssp. Bulgaricus (B3). Kafkas Univ Vet Fak Derg, 16, 81-86, 2010.
27. Ozbaş ZY, Aytaç SA: Behavior of Yersinia enterocolitica and Aeromonas hydrophila in skim milk during fermentation by various lactobacilli. Z Lebensm Unters Forsch, 202, 324-328, 1996.
28. Aroutcheva A, Gariti D, Simon M, Shott S, Faro J, Simoes JA, Gurguis A, Faro S: Defense factors of vaginal lactobacilli. Am J Obstet Gynecol, 185, 375-379, 2001.
29. Yuksekdag ZN, Beyatli Y, Aslim B: Determination of some characteristics coccoid forms of lactic acid bacteria isolated from Turkish kefirs with natural product. LWT/Food Sci Tech, 37, 663-667, 2004b.
30. Kim TS, Hur JW, Yu M, Chenigh CI, Kim KN, Hwang JK, Pyun YR: Antagonsim of Helicobacter pylori by bacteriocins of lactic acid bacteria. J Food Protect, 66, 3-12, 2003.
31. Midola PD, Korman MG, Turdinge JD, Lambert JR: Helicobacter pylori resistance to tetracycline. Lancet, 347, 1194-1195, 1996.