Boz Irk Sığırın Et Gevrekliğini Belirlemede CAST ve CAPN1 Genlerindeki Kimi SNP'lerin Kullanımı

Bu çalışmanın amaçı saf Boz ırk sığırın (TGC) kimi genotipik özellikleri ile et gevreklik özelliği arasındaki ilişkiyi araştırmaktır. Kesim sonrası sığır etlerinde artan kalsiyum aktivitesi ile et gevrekliği arasında doğrusal bir ilişki vardır. Kalpastatin (CAST), memeli dokularında bulunan kalpain'in (CAPN1) nötr bir proteaz inhibitörüdür. Sığır CAST ve CAPN1 genlerinde üç polimorfik yer (UoG-CAST, CAPN1 316 ve 4751) et kalite markörü olarak bilinir. Saf TGC'ın longissimus dorsi (LD) kası gevreklik özellikleri ile 3 et kalite markörünün ilişkileri araştırılıp tartışılmıştır. Genotipleri tanımlamada PCR-RFLP ve ARMS-PCR metotları kullanılmıştır. Etin su tutma kapasitesi (WHC), pişme kaybı (CL) ve tekstürünü (SF) belirlemek için LD kası örnekleri üzerinde çalışılmıştır. Extansif koşullarda yetiştirilen iki yaşındaki TGC sığırların SF ortalama ve standart hataları düveler de 4.339±0.217 kg/cm2, erkekler de 4.689±0.569 kg/cm2 olduğu saptanmıştır. Çalışılan et örneklerinde UoG-CAST (C/G), CAPN1 316 (C/G) ve CAPN1 4751(C/T) polimorfizmlerinin her iki alleli de gözlenmiştir. Et gevrekliği üzerine olumlu etkisi olan genotiplerin, kas liflerini kesmek için ortalama CAST-CC için 3.943±0.441 kg/cm2, CAPN1 4751-CC için 4.537±1.666 kg/cm2 ve CAPN1 316-GC için 3.869±0.721 kg/cm2 SF uygulanmıştır. Örneklerde CAPN1 316-CC genotipinde sığır belirlenmemiştir. Dişi ve erkek sığırlara ait tüm örneklerin dahil olduğu WHC, CL ve SF ortalama ve standart hata değerleri sırasıyla; %11.693±0.761, %26.952±0.636 ve 4.483±0.252 kg/cm2 olarak belirlenmiştir. Sonuç olarak, safkan TGC da et gevrekliğine özgü SNP markörlerin genetik çeşitliliğin olması, daha gevrek etli TGC sığır yetiştirme sürecine katkıda bulunabilir.

The Use of Various SNPs in CAST and CAPN1 Genes to Determine the Meat Tenderness in Turkish Grey Cattle

The aim of this study is to investigate the relationship between some genotypic characteristics of purebred Turkish Grey Cattle (TGC) and beef tenderness characteristics. There is a linear relationship between meat tenderness and the increasing calcium activity after slaughtering. Calpastatin (CAST) is a neutral protease inhibitor of Calpain (CAPN1) in mammalian tissues. The three polymorphic places in the CAST and the CAPN1 genes in cattle (UoG-CAST, CAPN1 316 and 4751) are known as the markers of beef quality. The relationship between the tenderness traits of the longissimus dorsi (LD) and the 3 beef quality markers (3 SNPs) in pure TGC have been investigated and discussed. PCR-RFLP and ARMS-PCR methods were utilized to identify the genotypes. In order to determine the water holding capacity (WHC), cooking loss (CL) and the shear force (SF), samples extracted from LD were probed. The average and standard error SF of a two-year-old TCG bred in extensive conditions was 4.339±0.217 kg/cm2 for the heifers and 4.689±0.569 kg/cm2 for bulls. Both alleles of the UoG-CAST (C/G), the CAPN1 316 (C/G) and the CAPN1 4751(C/T) polymorphisms in the samples were observed. The average SF of 3.943±0.441 kg/cm2, 4.537±1.666 kg/cm2, and 3.869±0.721 kg/cm2 were used for the CAST-CC, the CAPN1 4751-CC and the CAPN1 316-GC, respectively in order to cut the muscle fibers of the genotypes that have a positive effect on tenderness. No cattle of the CAPN1 316-CC genotype was identified among the samples. The average and standart error WHC, CL and SF values for the entire sample including heifers and bulls were found as 11.693±0.761%, 26.952±0.636%, and 4.483±0.252 kg/cm2, respectively. In conclusion, the presence of genetic variation in specific SNP markers of beef tenderness in purebred TGC can contribute to the process of raising TGC with more tender meat.

Kaynakça

Miller M, Huffman K, Gilbert S, Hamman L, Ramsey C: Retail consumer acceptance of beef tenderized with calcium chloride. J Anim Sci, 73 (8): 2308-2314, 1995. DOI: 10.2527/1995.7382308x

Koohmaraie M, Whipple G, Kretchmar D, Crouse J, Mersmann H: Postmortem proteolysis in longissimus muscle from beef, lamb and pork carcasses. J Anim Sci, 69 (2): 617-624, 1991. DOI: 10.2527/1991.692617x

Smith T, Thomas M, Bidner T, Paschal J, Franke D: Single nucleotide polymorphisms in Brahman steers and their association with carcass and tenderness traits. Genet Mol Res, 8 (1): 39-46, 2009. DOI: 10.4238/vol8- gmr537

Curi RA, Chardulo LAL, Mason M, Arrigoni M, Silveira AC, De Oliveira H: Effect of single nucleotide polymorphisms of CAPN1 and CAST genes on meat traits in Nellore beef cattle (Bos indicus) and in their crosses with Bos taurus. Anim Genet, 40 (4): 456-462, 2009. DOI: 1111/j.1365-2052.2009.01859.x

Schenkel F, Miller S, Jiang Z, Mandell I, Ye X, Li H, Wilton J: Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. J Anim Sci, 84 (2): 291- , 2006. DOI: 10.2527/2006.842291x

Van Eenennaam A, Li J, Thallman R, Quaas R, Dikeman M, Gill C, Franke D, Thomas M: Validation of commercial DNA tests for quantitative beef quality traits. J Anim Sci, 85 (4): 891-900, 2007. DOI: 10.2527/jas.2006-512

Casas E, White S, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase C, Johnson D, Smith T: Effects of and ?-markers in beef cattle on tenderness traits. J Anim Sci, 84 (3): 520-525, 2006. DOI: 2527/2005.83113x

Allais S, Journaux L, Levéziel H, Payet-Duprat N, Raynaud P, Hocquette J-F, Lepetit J, Rousset S, Denoyelle C, Bernard-Capel C: Effects of polymorphisms in the calpastatin and µ-calpain genes on meat tenderness in 3 French beef breeds. J Anim Sci, 89 (1): 1-11, 2011. DOI: 2527/jas.2010-3063

Page B, Casas E, Quaas R, Thallman R, Wheeler T, Shackelford S, Koohmaraie M, White S, Bennett G, Keele J: Association of markers in the bovine gene with meat tenderness in large crossbred populations that sample influential industry sires. J Anim Sci, 82 (12): 3474-3481, 2004. DOI: 10.2527/2004.82123474x

Li X, Ekerljung M, Lundström K, Lundén A: Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden. Meat Sci, 94 (2): 153-158, 2013. DOI: 10.1016/j. meatsci.2013.01.010

Curi RA, Chardulo LAL, Giusti J, Silveira AC, Martins CL, de Oliveira HN: Assessment of GH1, CAPN1 and CAST polymorphisms as markers of carcass and meat traits in Bos indicus and Bos taurus X Bos indicus cross beef cattle. Meat Sci, 86 (4): 915-920, 2010. DOI: 10.1016/j. meatsci.2010.07.016

Casas E, White S, Riley D, Smith T, Brenneman R, Olson T, Johnson D, Coleman S, Bennett G, Chase C: Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in cattle. J Anim Sci, 83 (1): 13- , 2005. DOI: 10.2527/2006.843520x

Corva P, Soria L, Schor A, Villarreal E, Cenci MP, Motter M, Mezzadra C, Melucci L, Miquel C, Paván E: Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina. Genet Mol Biol, 30 (4): 1064-1069, 2007. DOI: 10.1590/ S1415-47572007000600006

White S, Casas E, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase C, Johnson D, Keele J, Smith T: A new single nucleotide polymorphism in extends the current tenderness marker test to include cattle of and crossbred descent. J Anim Sci, 83 (9): 2001-2008, 2005. DOI: 2527/2005.8392001x

Honikel KO: Reference methods for the assessment of physical characteristics of meat. Meat Sci, 49 (4): 447-457, 1998. DOI: 10.1016/ S0309-1740(98)00034-5

Karakaya M, Saricoban C, Yilmaz M: The effect of mutton, goat, beef and rabbit-meat species and state of rigor on some technological parameters. J Muscle Foods, 17 (1): 56-64, 2006. DOI: 10.1111/j.1745- 2006.00035.x

Rincon G, Medrano J: Assays for genotyping single nucleotide polymorphisms in the bovine CAPN1 gene. Anim Genet, 37 (3): 294-295, DOI: 10.1111/j.1365-2052.2006.01430.x

Quaas R, Li J, Thallman R, Van Eenennaam A, Fernando R, Gill C: Validation of commercial DNA tests for quantitative beef traits. 8th World Congress on Genetics Applied to Livestock Production, August 13-18, 2006,

Belo Horizonte, Brasil Reardon W, Mullen A, Sweeney T, Hamill R: Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus. Meat Sci, 86 (2): 270-275, 2010. DOI: 10.1016/j.meatsci.2010.04.013

Ağaoğlu ÖK, Akyüz B, Kul BC, Bilgen N, Ertuğrul O: Genetic polymorphism of five genes associated with meat production traits in five cattle breeds in Turkey. Kafkas Univ Vet Fak Derg, 21, 489-497, 2015. DOI: 9775/kvfd.2014.12722

Kök S, Atalay S, Eken HS, Savaşçi M: The genetic characterization of Turkish Grey Cattle with regard to UoG-CAST, CAPN1 316 and CAPN1 markers. Pakistan J. Zool, 49 (1): 281-287, 2017. DOI: 10.17582/ journal.pjz/2017.49.1.281.287

Gill JL, Bishop SC, McCorquodale C, Williams JL, Wiener P: Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired beef cattle. Genet Sel Evol, 41 (1): 36, 2009. DOI: 10.1186/1297-9686-41-36

Kök S, Atalay S, Savaşçı M, Eken HS: Characterization of calpastatin gene in purebred and crossbred Turkish Grey Steppe cattle. Kafkas Univ Vet Fak Derg, 19 (2): 203-206, 2013. DOI: 10.9775/kvfd.2012.7470

Chung H, Shin S, Chung E: Effects of genetic variants for the bovine calpain gene on meat tenderness. Mol Biol Rep, 41 (5): 2963-2970, 2014. DOI: 10.1007/s11033-014-3152-3

Calvo J, Iguácel L, Kirinus J, Serrano M, Ripoll G, Casasşs I, Joy M, Pérez-Velasco L, Sarto P, Albertí P: A new single nucleotide polymorphism in the calpastatin (CAST) gene associated with beef tenderness. Meat Sci, 96 (2): 775-782, 2014. DOI: 10.1016/j. meatsci.2013.10.003

Frylinck L, Van Wyk G, Smith T, Strydom P, van Marle-Köster E, Webb E, Koohmaraie M, Smith M: Evaluation of biochemical parameters and genetic markers for association with meat tenderness in South African feedlot cattle. Meat Sci, 83 (4): 657-665, 2009. DOI: 10.1016/j. meatsci.2009.07.016

Lee SH, Kim SC, Chai HH, Cho SH, Kim HC, Lim D, Choi BH, Dang CG, Sharma A, Gondro C: Mutations in calpastatin and ?-calpain are associated with meat tenderness, flavor and juiciness in Hanwoo (Korean cattle): Molecular modeling of the effects of substitutions in the calpastatin/?-calpain complex. Meat Sci, 96 (4): 1501-1508, 2014. DOI: 1016/j.meatsci.2013.11.026

Soysal D: Determination of fattening performance, carcass characteristics and meat quality in Turkish Grey Cattle. PhD Thesis, Namik Kemal University, Institute of Science, 2012 (in Turkish).

Yüksel S, Yanar M, Aksu MI, Kopuzlu S, Kaban G, Sezgin E, Oz F: Effects of different finishing systems on carcass traits, fatty acid composition, and beef quality characteristics of young Eastern Anatolian Red bulls. Trop Anim Health Prod, 44 (7): 1521-1528, 2012. DOI: 10.1007/ s11250-012-0098-0

Özlütürk A, Esenbuğa N, Yanar M, Uenlue N, Macit M, Kopuzlu S: The effect of duration of finishing period on the performance, slaughter, carcass, and beef quality characteristics of Eastern Anatolian Red bulls. Turk J Vet Anim Sci, 32 (6): 441-448, 2008.

Preziuso G, Russo C, D'Agata M: Quality of carcass and meat in bullock of different genotypes raised under organic system. Annali Della Facoltà di Medicina Veterinaria, 57, 265-274, 2004.

Christensen M, Ertbjerg P, Failla S, Sañudo C, Richardson RI, Nute GR, Olleta JL, Panea B, Albertí P, Juárez M: Relationship between collagen characteristics, lipid content and raw and cooked texture of meat from young bulls of fifteen European breeds. Meat Sci, 87 (1): 61- , 2011. DOI: 10.1016/j.meatsci.2010.09.003

Costello S, O'Doherty E, Troy D, Ernst C, Kim K-S, Stapleton P, Sweeney T, Mullen A: Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus dorsi. Meat Sci, 75 (4): 551-557, 2007. DOI: 10.1016/j. meatsci.2006.06.021

Ciobanu DC, Bastiaansen JW, Lonergan SM, Thomsen H, Dekkers JC, Plastow GS, Rothschild MF: New alleles in calpastatin gene are associated with meat quality traits in pigs. J Anim Sci, 82 (10): 2829-2839, DOI: 10.2527/2004.82102829x

Kaynak Göster

  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995

2.3b 1.8b