Lohmann Kahverengi Tavuklarda Melanocortin Reseptörü Geninde Tek Nükleotid Polimorfizmi İle Yumurta Verim Özellikleri Arasındaki İlişki

Melanocortin reseptörü (MC4R) merkezi melanocortin sisteminde ve kuşlarda yem tüketiminin düzenlenmesinde önemli rol oynar. Bu çalışmada melanocortin geninin (MC4R) Lohmann Kahverengi tavuklarda yumurta verim özellikleri (sayı ve kilo) üzerine etkili bir gen olabileceği düşünülmüştür. MC4R genini amplifiye etmek amacıyla kan örneklerinden DNA ekstrakte edildi ve PCR ürünlerinin sekanslaması yapıldı. Her bir grupta sekans verisinin dizilimi MC4R-1'in nükleotid 22'sinde (T-G) (Sense mutasyon) yüksek yumurta ağırlığı için varyasyon bulunduğunu ortaya koydu. AB genotipine sahip tavuklar AA genotipine sahip tavuklar ile karşılaştırıldığında istatistiki olarak daha yüksek yumurta ağırlığına sahiptiler. Bu mutasyonun yumurta sayısı üzerine bir etkisi bulunmamaktaydı. Tespit edilen mutasyon ve ortalama yumurta üretimi analizi MC4R polimorfizmi ile yumurta ağırlığı arasında anlamlı bir ilgi bulunduğunu gösterdi. MC4R-SNP özellikle yumurta ağırlığı bakımından önemli bir belirteç olarak kullanılabilir.

Association of Single Nucleotide Polymorphism in Melanocortin Receptor Gene with Egg Production Traits in Lohmann Brown Chickens

Melanocortin receptor (MC4R) plays an important role in central melanocortin system and regulation of feed intake in birds. In this study the melanocortin gene (MC4R) was proposed as a candidate gene for egg production traits (yield and weight) in Lohmann Brown hens. DNA from blood samples was extracted to amplify the MC4R gene and the purified PCR products were sequenced. Alignment of sequence data from each group revealed that there is a variation detected in MC4R-1 at nucleotide 22 (T-G) (sense mutation) for high egg weight. Hens with the AB genotype produced significantly higher egg weight compared to hens with the AA genotype. There was no significant effect of this mutation on egg yield. The detected mutation and the analysis of egg production means revealed a significant association between MC4R polymorphism and egg weight. The MC4R-SNP could be considered as a useful marker in chicken selection especially for egg weight.

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