Abdolmohammad 3 KAJBAFZADEH, Pargol Ghavam 2 MOSTAFAVI, ? Nikta 1 MANSOURI, Hamidreza FATTAHIAN, Kimia 1 MANSOURI

Tavşanlarda Mürekkepbalığı Kabuğu ve Kan Pulcuğundan Zengin Plazma İle Birlikte Mürekkep Balığı Kaynaklı Hidroksiapatitin Tibial Kemik Hasarının İyileştirilmesindeki Rolü: Deneysel Bir Çalışma

Günümüzde deniz ürünleri kaynaklı doku yapı iskeleleri, biyouyumlu ve yüksek yenilenme potansiyeline sahip olmaları nedeniyle popülerdirler. Mürekkep balığının iç kabuğu olan Mürekkepbalığı kabuğundan elde edilmiş hidroksiapatit ile yapılmış önceki çalışmalarda kabuğun biyouyumluluğu ve biyoaktivitesi özellikle in vivo olmak üzere tam olarak çalışılmamıştır. Bu çalışmanın amacı, mürekkep balığı kaynaklı hidroksiapatitin in vivo potansiyelini ve bu doku iskelesi ile kan pulcuğundan zengin plazmanın kemik iyileşmesini etkilemedeki sinerjik etkisini araştırmaktır. Hidroksiapatit mürekkepbalığı (Sepia officinalis) kabuğundan hidrotermal transformasyon ile elde edildi. Hazırlanan materyalin dönüştürme ve mikroyapısı Tarayıcı elektron mikroskop (SEM) ve x ışını kırımı (XRD) analizi ile kontrol edildi. On beş erkek beyaz Yeni Zelanda tavşanı rastgele olarak her birinde 5 bacak olacak şekilde toplam 6 gruba ayrıldı. Hayvan sayısını azaltmak adına sağ ve sol arka bacakları ayrı gruplar olarak kullanıldı. Proksimal tibiada bilateral olarak tüm katman biyokortikal hasar oluşturuldu. Negatif kontrol grubunda hasar tedavi edilmeksizin bırakıldı. Oluşturulan hasar I'den V'e kadar olan deneysel gruplarda sırasıyla kan pulcuğundan zengin plazma, işlenmemiş mürekkepbalığı kabuğu, işlenmemiş mürekkepbalığı kabuğu ile birlikte kan pulcuğundan zengin plazma, mürekkepbalığı kaynaklı hidroksiapatit ve mürekkepbalığı kaynaklı hidroksiapatit ile birlikte kan pulcuğundan zengin plazma ile dolduruldu. 56. günde alınan örneklerde histopatolojik inceleme gerçekleştirildi. Kemik iyileşmesi birleşme, spongioz, korteks ve kemik iliği belirteçleri göz önüne alınarak değerlendirildi. Elde edilen sonuçlar, göz önüne alınan belirteçler yönünden Grup I'in negatif kontrole göre daha üstün olduğunu gösterdi. Grup IV ve V'de grup II ve III ile karşılaştırıldığında birleşme ve korteks belirteçleri bakımından tercih edilebilir sonuçlar oluştuğu gözlemlendi. Tüm gruplarda kabul edilebilir düzeyde spongioz oluşumun gerçekleştiği gözlemlendi. Mürekkepbalığı kaynaklı hidroksiapatit kemik oluşumunu uyarmak ve kemik rejenerasyonunu geliştirmede uygun bir biyomateryal olabilir. Ayrıca kan pulcuğundan zengin plazmanın kemik iliği oluşumunu geliştirmede başarılı olduğu gözlemlendi.

The Role of Cuttlebone and Cuttlebone Derived Hydroxyapatite with Platelet Rich Plasma on Tibial Bone Defect Healing in Rabbit

Today marine-derived biologic scaffolds are popular due to their biocompatibility and high regenerative potential. Previous studies prepared hydroxyapatite from cuttlebone, the internal shell of cuttlefish; However, its biocompatibility and bioactivity has not been fully studied especially in-vivo. The aim of this study was to evaluate cuttlebone-derived hydroxyapatite in-vivo potential and possible synergistic effect of platelet rich plasma with this scaffold in promoting bone healing. Hydroxyapatite was prepared from cuttlebone (Sepia officinalis) via hydrothermal transformation. The conversion and microstructure of prepared material was assessed by scanning electron microscopy (SEM) and x-ray diffraction (XRD) analysis. Fifteen male white New Zealand rabbits were randomly divided to 6 groups each containing 5 limbs. In order to reduce sample size right and left pelvic limbs of rabbits were used as separate groups. Full thickness bi-cortical defects were created bilaterally in proximal tibia. The defect was left untreated in negative control group. In experimental group I to V the defect was filled with platelet rich plasma, raw cuttlebone, raw cuttlebone combined with platelet rich plasma, cuttlebone derived hydroxyapatite and cuttlebone derived hydroxyapatite combined with platelet rich plasma, respectively. Histopathological evaluation was performed on specimens received on day 56. Bone healing was assessed according to union, spongiosa, cortex and bone marrow indices. Our results demonstrated that Group I was superior to negative control group in defined indices. Groups IV and V showed preferable outcomes regarding to union and cortex indices in comparison to groups II and III. Also acceptable degree of spongiosa formation was observed in all groups. Cuttlebone derived hydroxyapatite could be an appropriate biomaterial to stimulate bone formation and enhance bone regeneration. Furthermore platelet rich plasma was successful in advancement of bone marrow formation.

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