Funda KIRAL, Gamze Sevri 2 EKREN AŞICI, Merve KAYKI, Murat YILMAZ

Farklı Yaşlardaki Saanen Keçilerinin Farkli Mevsimlerdeki HSP 60 ve HSP70 İçin mRNA Gen Ekspresyon Profilleri

Bu çalışmada, farklı yaşlardaki Saanen keçilerinin bahar mevsimine göre yaz, sonbahar ve kış mevsimlerindeki HSP60 ve HSP70 mRNA gen ekspresyon düzeyleri karşılaştırılmıştır. Hayvan materyali olarak sağlıklı 18 baş Saanen keçisi kullanılmıştır. Hayvanlar yaşlarına göre üç gruba ayrılmıştır. Birinci grup hayvanlar; 1-2 yaşında, ikinci grup; 3-4 yaş, üçüncü grup hayvanlar; 5-6 yaşındadır. Genel olarak, bölgede keçiler için en uygun mevsim olan ilkbahardaki HSP60 ve HSP70 mRNA gen ekspresyon düzeyleri yaz, sonbahar ve kış aylarında yaşlara göre karşılaştırılmıştır. Tüm gruplar için HSP60 ve HSP70 mRNA ekspresyon düzeyleri yaz için istatistiksel olarak önemsiz bulunmuştur. Kış mevsimde HSP60 mRNA gen ekspresyon düzeyleri gruplar arasında karşılaştırıldığında birinci grup ve diğer iki grup arasındaki fark istatistiki olarak önemli bulunmuştur (P

The mRNA Gene Expression Profiles for HSP60 and HSP70 in Various Aged Saanen Goats in Different Seasons

In this study, the mRNA gene expression levels for HSP60 and HSP70 in various aged Saanen Goats in the spring were compared with the summer, fall and winter levels. The animal material was constituted by healthy 18 Saanen Goats at various ages. The animals were divided into three groups so that group I contained goats at 1-2 years old; group II 3-4 year- old-ones and group III 5-6 year-olds. In general, HSP60 and HSP70 mRNA expression levels were determined to have increased in all groups in the summer, fall and winter when compared with the levels in the spring which is known to be the most convenient season for goats in the region. The differences of mRNA expression levels for HSP60 and HSP70 among groups in the summer were found statistically insignificant. When the HSP60 expression levels in the winter in all groups were compared, the difference between Group I and the other two groups, Group II and III, was found statistically significant (P<0.05). The levels were seen to be higher in the young goats than in the old ones. This finding was that most significant aspect of this study. In other words, these levels decreased as the age increased. In resisting heat stres, to be able to define the roles and the functional mechanisms of mRNA gene expression levels of HSP60 and HSP70 in goats having high adaptation capacities to the environment particularly according to ages could be regarded as facilitators for the studies to be done on all species. In the following years, HSPs are believed to play significant roles in the selection of resistant animals particularly to the environmental conditions and to be one of the significant physiological parameters which will focus on farm animals.

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