YALÇIN AKBULUT, Seyit Ali BİNGÖL, Turgay DEPREM, Serap KORAL TAŞÇI 1

Şahin (Buteo buteo) İnce Bağırsak ve Karaciğerinin Anatomik ve Histolojik Yapısı İle Katalazın İmmunohistokimyasal Lokalizasyonu

Bu çalışmada şahinlerin (Buteo buteo) ince bağırsakları ile karaciğerlerinin genel yapısı ve bu dokularda katalazın immunohistokimyasal lokalizasyonunun incelenmesi amaçlandı. Histolojik incelemeler için dokulara üçlü boyama ve periyodik asit Shiff (PAS) boyaması yapıldı. Dokulardaki katalaz enziminin immunohistokimyasal lokalizasyonunun belirlenmesi için Avidin-Biotin-Peroksidaz Kompleks (ABC) tekniği uygulandı. Şahin ince bağırsağı ve karaciğerinin genel histolojik yapısı incelendiğinde diğer kanatlı türlerine benzer olduğu görüldü. Katalaz immunoreaktivitesinin ince barsaklarda lamina propria ve submukoza katmanlarında, karaciğerde ise hepatositlerin sitoplazmalarında olduğu görüldü. Antioksidan bir enzim olan katalazın ince bağırsak ve karaciğerde belirtilen yerlerde olması bu bölgelerin antioksidan savunmanın gerçekleştiği yer olduğunu düşündürmektedir.

The Anatomical and Histological Structures of Buzzard's (Buteo buteo) Small Intestine and Liver, and Immunohistochemical Localization of Catalase

This study aimed to examine the structure of the buzzard's Buteo buteo) small intestine and liver, and the localization of catalase in those organs. Crossman's modified triple stain and Periodic Acid Schiff (PAS) stain were applied to the samples for histological examination. To detect catalase localization in the tissues, the avidin-biotin-peroxidase complex (ABC) technique was used. It was observed that the general histological structure of the buzzard's small intestine and liver was similar to that of other avian species. Catalase immunoreactivity in the lamina propria and submucosa in the small intestine were also observed, as well as catalase immunoreactivity in the hepatocyte cytoplasm in the liver. Some areas in the small intestine and liver had catalase immunoreactivity, which means that an antioxidant defence occurs in those areas.

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