Engin BERBER, Şükrü TONBAK, Mehmet ÇABALAR

Türkiye’nin bazı bölgelerinde 2008 yılında görülen bovine ephemeral fever virüs enfeksiyonlarının polimeraz zincir reaksiyonuyla belirlenmesi

Bovine ephemeral fever (BEF), biyokimyasal ve hematolojik değişikliklerle karakterize sığırların viral bir hastalığıdır. Süt veriminde azalmaya ve besi sığırcılığında verim kaybına neden olmasından dolayı ekonomik olarak önemli bir hastalıktır. Bu çalışmada Doğu Anadolu, Güneydoğu Anadolu ve Akdeniz bölgelerinde yer alan on vilayetten 2008 yılında 110 adet kan ve 120 adet serum örneği toplanmıştır. Toplanan örneklerden izole edilen RNA’lara tersine transkripsiyon ve polimeraz zincir reaksiyon (RT-PZR) deneyleri yapıldı. Glikoprotein genine spesifik primerlerin kullanıldığı polimeraz zincir reaksiyonunda 2008 yılında 20 örnek pozitif olarak bulundu.

Detection of bovine ephemeral fever virus infections by polymerase chain reaction in some regions of Turkey in 2008

Bovine ephemeral fever (BEF) is a viral disease of cattle which is characterized by hematological and biochemical changes. It can cause significant economic impact due to reduced milk production in dairy herds, and loss of condition in beef cattle. In the present study, a total of 110 whole blood and 120 sera were collected from cattle in 10 provinces located in Southeastern Anatolia, Eastern Anatolia, and Mediterranean region in 2008. After total RNA extraction of the samples, reverse transcriptase polymerase chain reaction (RT-PCR) was used to recover partial sequences of the BEFV glycoprotein gene. Glycoprotein gene was found in 20 samples by polymerase chain reaction by using specific primers in 2008.

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1. Nandi S, Negi BS. Bovine ephemeral fever: a review. Comp Immunolo Microbiol Infect Dis 1999; 22: 81-91.
2. Venter GJ, Hamblin C, Paweska JT. Determination of the oral susceptibility of South African livestock-associated biting midges, Culicoides species, to bovine ephemeral fever virus. Med Vet Entomol 2003; 17: 133-137.
3. Dhillon J, Cowley JA, Wang Y, Walker PJ. RNA polymerase (L) gene and genome terminal sequences of ephemeroviruses bovine ephemeral fever virus and adelaide river virus indicate a close relationship to vesiculoviruses. Virus Res 2000; 70: 87-95.
4. Kongsuwan K, Cybinski DH, Cooper J, Walker PJ., Location of neutralizing epitopes on the G protein of bovine ephemeral fever rhabdovirus. J Gen Virol 1998; 79: 2573- 2581.
5. Liao YK, Inaba Y, Li NJ, et al. Epidemiology of bovine ephemeral fever virus infection in Taiwan. Microbiol Res 1998; 153: 289-295.
6. McWilliam SM, Kongsuwan K, Cowley JA, Byrne KA, Walker PJ. Genome organization and transcription strategy in the complex GNS-L intergenic region of bovine ephemeral fever rhabdovirus. J Gen Virol 1997; 78: 1309- 1317.
7. Walker PJ, Byrne KA, Riding GA, et al. The genome of bovine ephemeral fever rhabdovirus contains two related glycoprotein genes. Virology 1992; 191: 49-61.
8. Walker PJ, Cybinski DH. Proteins of bovine ephemeral fever virüs. J Gen Virol 1991; 72: 67-74.
9. Holmes IH, Doherty RL. Morphology and Development of bovine ephemeral fever virus. J Virol 1970; 5: 91-96.
10. Yeruham I, Braverman Y, Yadin H, et al. Epidemiological investigations of outbreaks of bovine ephemeral fever in Israel. Vet Rec 2002; 151: 117-121.
11. Yeruham I, Ham MV, Bar D, Yadin H, Tiomkin D. Economic aspects of the 1999 outbreak of bovine ephemeral fever in dairy cattle herds in the Jordan Valley in Israel. Vet Rec 2003; 153: 180-182.
12. Yeruham I, Yadin H, Abramovitch I, Shama Z. Bovine ephemeral fever in a dairy cattle herd in the Jordan Valley. Vet Rec 2005; 156: 284-286.
13. Stram Y, Kuznetzova L, Levin A, Yadin H, Rubinstein-Giuni M. A real-time RT-quantative(q) PCR for the detection of bovine ephemeral fever virus. J Virol Methods 2005; 130: 1-6.
14. Yeruham I, Sharir B, Yadin H, Tiomkin D, Chai D. Bovine ephemeral fever in beef cattle herds in the Jordan Valley, Israel. Vet Rec 2003; 152: 86-88.