Elazığ, Malatya ve Tunceli illerinde koyunlarda görülen abort vakalarında pestivirusların rolünün araştırılması

Bu çalışmada; Elazığ, Malatya ve Tunceli illerinde koyunlardan elde edilen abort örneklerinden pestivirüs (Border disease; BD ve bovine viral diarrhoea virus; BVDV) prevalansının antijen enzim linked immunosorbent assay (ELISA) ile belirlenmesi amaçlanmıştır. Toplam 160 örneğin ELISA ile çalışılması neticesinde örneklerin 36 (%22.5)’sı pestivirüs antijen varlığı yönünden pozitif tespit edilmiştir. Sonuç olarak, pestivirüslerin Elazığ, Malatya ve Tunceli illerindeki koyunlarda abortların etiyolojisinde önemli oldukları belirlenmiştir.

Investigation of the role of pestiviruses in abort cases of sheep at Elazığ, Malatya and Tunceli Provinces

The present study was aimed for determining the prevalance of pestiviruses (Border disease; BD and bovine viral diarrhoea virus; BVDV) by enzyme-linked immunosorbent assay (ELISA) method in samples collected from abort cases in sheep in Elazığ, Malatya and Tunceli. As a result of this study, 36 (22.5%) of 160 animals were found positive for pestivirus. The results inidcates that, pestiviruses are important agent in etiology of sheep abortion in Elazığ, Malatya and Tunceli provinces.

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1. García-Pérez AL, Minguijón E, Barandika JF, et al. Detection of Border disease virus in fetuses, stillbirths, and newborn lambs from natural and experimental infections. J Vet Diagn Invest 2009; 21: 331-337.
2. Givens MD, Marley MS. Infectious causes of embryonic and fetal mortality. Theriogen 2008; 70: 270-285.
3. Menzies PI. Control of important causes of infectious abortion in sheep and goats. Vet Clin North Am Food Anim Pract 2011; 27: 81-93.
4. Albayrak H, Gumusova SO, Ozan E, Yazici Z. Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey. Trop Anim Health Prod 2012; 44: 677-680.
5. Horner GW, Tham KM, Orr D, et al. Comparison of an antigen capture enzyme-linked assay with reverse transcription-polymerase chain reaction and cell culture immunoperoxidase tests for the diagnosis of ruminant pestivirus infections. Vet Microbiol 1995; 43: 75-84.
6. Hurtado A, Sanchez I, Bastida F, et al. Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny. Virol J 2009; 6: 189.
7. Kobayashi T, Yanase T, Yamakawa M, et al. Genetic diversity and reassortments among Akabane virus field isolates. Virus Res 2007; 130: 162-171.
8. Maclachlan NJ, Drew CP, Darpel KE, Worwa G. The pathology and pathogenesis of bluetongue. J Comp Path 2009; 141: 1-16
9. Burgu I, Oztürk F, Akca Y, et al. Investigations on the occurrence and impact of bovine viral diarrhea (BVD) virus infections in sheep in Turkey. Dtsch Tierarztl Wochenschr 1987; 94: 292-294.
10. Çetinkaya B, Öngör H, Muz A, et al. Detectian of brucella species DNA in the stomach content of aborted sheep fetuses by PCR. Vet Rec 1999; 144: 239-240.
11. Duman R, Durak Y. Investigations on chlamydia psittaci infections causing abortion in sheep in Konya district using complement fixation test. Turk J Vet Anim Sci 1998; 22: 511-516.
12. Erganiş O, Kaya O, Hadimli LL, Güler L. Rapid diagnosis of ovine brucella, campylobacter and salmonella infections from fetal stomach contents by coagglutination test. Small Rum Res 2002; 45: 123-127.
13. Gripshover EM, Givens MD, Ridpath JF, et al. Variation in Erns viral glycoprotein associated with failure of immunohistochemistry and commercial antigen capture ELISA to detect a field strain of bovine viral diarrhea virus. Vet Microbiol 2007; 125: 11-21.
14. Kalaycı Ş. SPSS Uygulamalı Çok Değişkenli İstatistik Teknikleri. 2. Baskı, Ankara: Asil Yayın, 2006.
15. Güler L, Hadımlı H, Erganiş O, et al. Field evaluation of a PCR for the diagnosis of chlamydial abortion in sheep. Vet Rec 2006; 159: 742-745.
16. Güler L, Gündüz K, Ok Ü. Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol 2003; 93: 53-61.
17. Gür S. A investigation of border disease virus in sheep in Western Turkey. Trop Anim Health Prod 2009; 41: 1409- 1412.
18. Muz A, Özer H, Eröksüz H, et al. Bacteriologic, serologic and pathologic studies on abortus cases of goats and sheep in Elazığ and its vicinity. Turk J Vet Anim Sci 1999; 23: 177-188.
19. Oguzoglu TC, Tan MT, Toplu N, et al. Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup? Vet Microbiol 2009; 135: 374-379.
20. Öngör H, Çetinkaya B, Açik MN, Karahan M, Bulut H. Detection of chlamydophila abortus in ovine milk by immunomagnetic separation-polymerase chain reaction. J Vet Med B 2004; 50: 43-45.
21. Sareyyüpoğlu B, Diker S, Güngördü S. Koyunlarda abortif bakteriyel infeksiyonların tanısında multiplex-PCR tekniklerinin geliştirilmesi. TÜBİTAK TOVAG Proje 104O237 2008: 1-61.
22. Hisarcioglu S, Kale M, Acar A. Investigation of pestiviruses infections in aborted sheep and goats in Burdur region. Kafkas Univ Vet Fak Derg 2009; 15: 163-167.
23. Azkur AK, Gazyagcı S, Aslan ME, Unal N. Molecular and serological characterization of pestivirus infection among sheep in Kirikkale, Turkey. Kafkas Univ Vet Fak Derg 2011; 17: 83-92.
24. İssi M, Gül Y, Gürçay M, Gök T. Elazığ yöresindeki koyunlarda saptanan pestivirus enfeksiyonu. FÜ Sağ Bil Vet Derg 2012; 26: 165-169.
25. Juliá S, Craig MI, Jiménez LS, Pinto GB, Weber EL. First report of BVDV circulation in sheep in Argentina. Prev Vet Med 2009; 90: 274-277.
26. Mishra N, Pitale SS, Rajukumar K, et al. Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India. Acta Virol 2012; 56: 209-215.