Mustafa ÖZKARACA, Aydın SAĞLIYAN, Cihan GÜNAY, Mehmet Cengiz HAN

Effect of autologous serum on healing of corneal endothelium in experimentally-ınduced alkaline burns of the cornea in rabbits

Bu çalışmada korneanın alkali yanıklarında otolog serumun kornea endoteli üzerindeki etkinliği araştırılmıştır. Alkali yanıklar korneada çok şiddetli travmalar oluştururlar. Bu nedenle iyileşmesi oldukça zor ve komplikasyonlu olur. Endotel tabaka korneanın şeffaflığının korumasında görevlidir. Endotel tabakada oluşacak hücre hasarının onarılması çevredeki sağlam hücrelerin hasarlı alana migrasyonu ile olmaktadır. Otolog serum kologenaz aktivitesini inhibe ederek endotel tabakasındaki hasarı azaltır ve hücrelerin migrasyonunu hızlandırır. Çalışmada 3 grupta toplam 21 adet Yeni Zelanda tavşanı kullanıldı. Genel anestezi altında II. ve III. gruplarda 2 N NaOH ile sağ gözlerde korneada alkali yanık oluşturuldu ve 21 gün süreyle II. grupta serum fizyolojik, III. grupta ise otojen serum günde 4 kez uygulandı. I. grupta ise alkali yanık oluşturulmadan 21 gün süreyle günde 4 kez otojen serum uygulandı. Yirmibirinci günün sonunda ötenazi edilen tavşanların korneaları çıkartılarak histopatolojik muayeneleri yapıldı. III. gruptaki endotel defekt alanları ve endotel hücre kaybının II. gruba göre daha az olduğu tespit edildi. Bu çalışmada otojen serumun kornea alkali yanıklarında endotel iyileşmesi üzerine yararlı etkisinin olduğu görüldü.

Tavşanlarda deneysel oluşturulan kornea alkali yanıklarında otojen serumun kornea endotel iyileşmesi üzerine etkisi

In this study, the effectiveness of autologous serum on corneal endothelium in alkaline burns of the cornea was investigated. Alkaline burns lead to severe trauma on the cornea. Cornea healing is quite difficult and complicated. Endothelial layer is responsible for preserving transparency of the cornea. Repair of the cell injury in the endothelial layer is achieved through migration of healthy cells to the injured area. Autologous serum reduces injury of the endothelial layer through inhibiting collagenase activity and accelerating cell migration. In the study, a total of 21 New Zealand rabbits were divided into 3 groups, 7 in each group. Alkaline burn was created in the right eyes with 2N NaOH in groups II and III under general anesthesia and saline solution was administered in group II and autologous serum was administered in group III four times a day for 21 days. In group I, autologous serum was administered four times a day for 21 th days without creating alkaline burn. At the end of 21 day, corneas of the decapitated rabbits were removed and histopathologically examined. Endothelial defect areas and endothelial cell loss were lower in group III compared to group II. In this study, it is suggested that the use of autologous serum was seen to be effective on the healing of the endothelium in the alkaline induced corneal burns.

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