Keklik (alectoris chukar) harder bezi üzerine histolojik ve histokimyasal çalışmalar

Bu çalışmada keklik (Alectoris chukar) Harder bezinin histolojik yapı ve histokimyasal özelliklerinin belirlenmesi amaçlandı. Servikal dislokasyon yöntemiyle ötenazi uygulanan 4 adet erişkin kekliğin (Alectoris chukar) Harder bezleri alındı. Uygulanan histokimyasal boyama yöntemleri sonucunda korpus glandulayı oluşturan hücrelerde karboksilatlı, O- sülfat esterli ve sülfatlı mukosubstansın güçlü yoğunlukta reaksiyon verdiği gözlendi. Primer ve merkezi kanal hücrelerinde asidik, karboksilatlı ve nötral mukosubstansın orta yoğunlukta olduğu belirlendi. Merkezi kanal mukus hücrelerinin mukosubstans içeriğinin ise asidik karakterde olduğu saptandı.

Histological and histochemical studies on the harderian gland of partridge (alectoris chukar)

In this study, it was aimed to determine histological structure and histochemical features of Harderian gland of partridge (Alectoris chukar). The Harderian glands of 4 adult partridge were taken after euthanasia by cervical dislocation method. As a result of the applied histochemical methods, it was observed that carboxylated, O-sulfated esters and sulfated mucosubstances gave strong reaction in the corpus glandula epithelial cells. While acidic, carboxylated and neutral mucosubstance were moderate in the primary and central. Mucosubstance content was acidic character in the central canal mucus cells.

PDF

___

1. Watanabe M. An autoradiographic biochemical and morphological study of the Harderian gland of Mouse. J Morph 1980; 163: 349-365.
2. Bayraktaroğlu AG, Ergün E. Histomorphology of the Harderian gland in the Angora rabbit. Anat Histol Embryol 2010; 39: 494-502.
3. Chieffi BG, Dimatteo L, Minucci S. The orbital glands of the chelonians Pseudemys scripta and Testudo graeca: comparative histology, histochemical and ultrastructural investigations. J Anat 1992: 180: 1-13.
4. Chieffi G, Baccari GC, Dimatteo L, et al. Cell biology of the Harderian gland. Int Rev Cytol 1992; 168: 1-80.
5. Brobby GW. On the Harderian gland of the duck (Anas platyrhynchus). Zeitsch Zellforsch 1992; 133: 223-230.
6. Burns RB, Maxwell MH. The structure of the Harderian gland and lacrimal gland ducts of the turkey, fowl and duck. A light microscope study. J Anat 1979; 128: 285-292.
7. Kozlu T, Akaydın Bozkurt Y, Altunay H, Karadağ Sarı E. Histological and histochemical studies on the Harderian gland of the osprey (Pandion haliaetus). J Anat 2010; 9: 1875-1879.
8. Rehorek SJ, Smith TD. The primate Harderian gland: Does it really exist. Ann Anat 2006; 188: 319-327.
9. Payne AP. The Harderian gland: a tercentennial review. J Anat 1994; 185: 1-49.
10. Olcese J, Wesche A. The Harderian gland. Comp Biochem Physiol 1989; 93: 655-665.
11. Sakai T, Yohro T. A histological study of the Harderian gland of Mongolian gerbils (Meriones meridianus). Anat Rec 1981; 100: 259-270.
12. Russel PH, Koch G. Local antibody forming cell responses to the Hitcher and Ulster strains of Newcastle disease virus. Vet Immunol Immunopathol 1989; 37: 165-180.
13. King AS, McLelland J. Birds their structure and function. 2nd Edition. London, Philadelphia: Bailliere Tindall, 1989.
14. Liman N. Duyu Sistemi. Özer A. (Editör). Veteriner Özel Histoloji. 3. Baskı, Ankara: Nobel Yayıncılık, 2011: 319- 320.
15. Altunay H, Kozlu T. The fine structure of the Harderian gland in the ostrich (Struthio camelus). Anat Histol Embryol 2004; 33: 141-145.
16. Dimitrov DS, Nikiforov IP. Histological and histochemical studies of harderian gland, lacrimal gland and bursa fabricius in mallard ducks (Anas sterilis) with chlamydial infection. Bulg J Vet Med 2005; 8: 119-127.
17. Kozlu T, Altunay H. Light and electron microscopic studies of the quail (Coturnix coturnix) Harderian gland. J Anat 2011; 10: 932-938.
18. Culling CFA, Reid PE, Dunn WL. A new histochemical method for the identification and visualization of both side chain acylated and non-acylated sialic acids. J Histochem Cytochem 1976; 24: 1225-1230.
19. McManus JFA. Histological and histochemical uses of periodic acid. Stain Technol 1948; 23: 99-108.
20. Lev R, Spicer SS. Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem 1964; 12: 309.
21. Mowry RW. Alcian blue tecniques fort he histochemical study of acidic carbohydrates. J Histochem Cytochem 1956; 4: 407-408.
22. Gomari G. Gomari’s aldehyde fuchsin stain. In: Culling CFA, Allison RT, Barr WT (Editors). Cellular Pathology Tecnique. London: Butterworths 1952: 238.
23. Spicer SS, Mayer DR. Aldehyde fuchsin/Alcian blue. In: Culling CFA, Allison RT, Barr WT (Editors). Cellular Pathology Tecnique. London: Butterworths 1960: 233.
24. Djeridane Y. The Harderian gland and its excretory duct in the Wistar rat. A histological and ultrastructural study. J Anat 1994; 184: 553-566.
25. Liman N, Gülmez N. Fransız Beyazı (Anser anser) ırkı kazlarda Harderian bezinin gelişimi üzerinde ışık mikroskobik incelemeler. Ankara Üniv Vet Fak Derg 1996; 43: 25-30.
26. Yaren FM. “Dişi sülün (Phasianus colchicus)’lerin Harder bezi üzerinde histolojik ve histokimyasal çalışmalar”. http://www.bcltechnologies. com/allpdf/ 15.12.2011.