Efe Kurtdede, Erdal Kara, Ufuk Kaya, Taha Burak Elifoğlu

Plasental retansiyonlu süt ineklerinde hepatokinlerin, proinflamatorik sitokinlerin, oksidatif stres ve enerji ile ilgili metabolizma analitlerinin ve hematolojik parametrelerin değerlendirilmesi

Amaç: Bu çalışmada, plasenta retansiyonlu (PR) ineklerde serum ANGPTL4, FGF21, IL-1β, IL-6, SOD, MDA düzeylerinin ve enerji ile ilgili metabolizma analitleri ve hematolojik parametrelerin araştırılması amaçlandı. Gereç ve Yöntem: Çalışmada 10 PR'li ineğin ve 10 sağlıklı kontrol ineğin (CC) koksigeal venlerinden kan örnekleri alındı. Tüm örnekler serum glikoz, esterleşmemiş yağ asitleri (NEFA), trigliserid, HDL, toplam protein, albümin, aspartat aminotransaminaz (AST) süper oksit dismutaz (SOD) ve malondialdehit (MDA) bakımından analiz edildi. Kan serumu hepatokinleri (anjiyopoietin benzeri protein 4 (ANGPTL4) ve fibroblast büyüme faktörü 21 (FGF21)) ve proinflamatuar sitokin yanıtına (IL-1 beta ve IL-6) ilişkin parametrelerin düzeyleri değerlendirildi. Bulgular: PR’li ineklerin karaciğer fonksiyonu ile ilgili olarak serum AST (p < 0.05) düzeyinde yükselme saptandı. Negatif enerji dengesine adaptasyon sürecinde serum NEFA düzeyinde yükselme ve serum HDL düzeyinde azalma ortaya çıktı (p < 0.05). Antioksidan sistemin durumunu yansıtan serum SOD (p < 0.001) ve MDA (p < 0.05) düzeylerinde önemli yükselme belirlendi. Serum IL-6 (p < 0.05), SOD ve MDA düzeylerindeki önemli artış ineklerde metabolik, inflamatorik ve oksidatif stresin etkin olarak ortaya çıktığının; serum ANGPTL4 ve FGF21 (p < 0.001) değerlerinin yüksek bulunmasının ise karaciğerin adaptasyonla başedebildiğinin göstergesi olarak yorumlandı. PR'li ineklerde monosit sayısı anlamlı olarak daha düşüktü (p

Evaluation of hepatokines, proinflammatory cytokines, oxidative stress and energy related metabolism analytes and hematological parameters in dairy cows with placental retention

Aim: To investigate the serum levels of angiopoietin-like protein 4 (ANGPTL4), fibroblast growth factor 21 (FGF21), interleukin 1 beta (IL-1β), IL-6, superoxide dismutase (SOD), malondialdehyde (MDA), and energy-related metabolism analytes and hematological parameters in cows with placental retention (PR). Materials and Methods: In the presented study, blood samples were taken from the coccygeal veins of 10 cows with PR and 10 healthy control cows. All of the samples were analyzed to determine the serum glucose, unesterified fatty acid (NEFA), triglyceride, high-density lipoprotein (HDL), total protein, albumin, aspartate aminotransaminase (AST), SOD, and MDA levels. Blood serum hepatokines, ANGPTL4, FGF21 levels, proinflammatory cytokine response parameters, IL-1 beta, and IL-6 levels were evaluated. Results: As a result, the increase in serum AST (p < 0.05) indicated altered liver function. During negative energy balance, the serum NEFA levels (p < 0.05) increased, and serum HDL levels (p < 0.05) decreased as indicators of an increased risk of metabolic pathology. Increases in the serum ANGPTL4, FGF21, CRP, and SOD (p < 0.001) and IL-6, MDA, creatine kinase levels (p < 0.05) were evaluated as indicators of the development of effective metabolic, inflammatory, and oxidative stress. The monocyte count was significantly lower (p < 0.05) in the cows with PR. Conclusion: It was concluded that significant increases in the serum ANGPTL4 and FGF2 levels were associated with energy balance, effective cytokine responses, and oxidative stress in dairy cows with PR.

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