Yasemin Öznurlu, Tuğba Özaydın, Emrah Sur

Streptozotosin ile diyabet oluşturulan ratlarda Koenzim Q 10’un testis dokusu üzerine etkileri

Gereç ve Yöntem: Çalışmada otuz dört yetişkin erkek Wistar Albino rat kullanılmıştır. Hayvanlar kontrol grubu (K), yağ grubu (Y; günlük 0,3 ml intraperitoneal mısır yağı enjeksiyonu), CoQ10 grubu (CoQ10; günlük 10 mg/kg CoQ10'un intraperitoneal enjeksiyonu), diyabet grubu (D; günde tek doz olmak üzere iki gün 40 mg/kg STZ subkutan enjeksiyonu) ve diyabet+CoQ10 grubu (D+ CoQ10; diyabet oluşturulan ratlara günlük 10 mg/kg CoQ10'un intraperitoneal enjeksiyonu) olmak üzere beş gruba ayrılmıştır. Dördüncü haftanın sonunda hayvanlar sakrifiye edilerek testis doku örnekleri alınmış ve tartılmıştır. Alınan doku örnekleri Bouin solüsyonunda tespit edilerek rutin histolojik tekniklerle işlenmiştir. Kesitler genel histolojik yapının belirlenmesi için H&E boyama yöntemi ile boyanmıştır. PCNA reaktivitesi ise immunohistokimyasal yöntemle belirlenmiştir. Bulgular: Yapılan çalışma sonucunda diyabet ve diyabet+CoQ10 gruplarında, kontrol, yağ ve CoQ10 gruplarına göre ortalama canlı ağırlığın önemli oranda azaldığı, gonadosomatik indeksin (GSI) ise yüksek olduğu gözlenmiştir (p

The effects of Coenzyme Q10 on testes of rats with diabetes induced by streptozotocin

Materials and Methods: In this study, thirty-four adult male Wistar Albino rats were used. The animals were divided into five groups: Control group (K), oil group (Y; daily intraperitoneal injection of 0,3 ml corn oil), CoQ10 group (CoQ10; daily intraperitoneal injection of 10 mg/kg CoQ10), Diabetes group (D; subcutan injection of 40 mg/kg STZ, single daily dose for two days), and Diabetes+ CoQ10 group (D+CoQ10; Diabetic rats was injected daily intraperitoneally 10 mg/kg CoQ10). At the end of fourth week, tissue samples were obtained from sacrified animals. Testes were weighed, then fixed in Bouin's solution and processed by means of routine histological techniques. Sections were stained with H&E staining method to determine the general histological structure. Also, PCNA reactivity was determined by immunohistochemical method. Results: The mean body weight was significantly decreased in diabetes and diabetes+ CoQ10 groups when compared to control, oil and CoQ10 groups but the gonadosomatic index (GSI) was found higher in diabetes groups (p

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Aktaş C, Kanter M, Erboğa M, Timurkan H, 2011. Effects of experimental diabetes on testis proliferations and apoptosis in rats . J Exp Clin Med, 28(3), 94-98.
Altay B, Cetinkalp S, Doganavşargil B, Hekimgil M et al., 2003. Streptozotocin-induced diabetic effects on spermatogenesis with proliferative cell nuclear antigen immunostaining of adult rat testis. Fertil Steril, 80(2), 828-31.
Ballester J, Muñoz MC, Domínguez J, Rigau T et al., 2004. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J Androl, 25(5), 706- 19.
Connolly KM, Bogdanffy MS, 1993. Evaluation of proliferating cell nuclear antigen (PCNA) as an endogenous marker of cell proliferation in rat liver: a dual-stain comparison with 5-bromo-2'-deoxyuridine. J Histochem Cytochem, 41(1), 1-6.
Corona G, Bianchini S, Sforza A, Vignozzi L et al., 2015. Hypogonadism as a possible link between metabolic diseases and erectile dysfunction in aging men. Hormones (Athens), 14(4), 569-78.
Corona G, Giorda CB, Cucinotta D, Guida P et al., 2014. Gruppo di studio SUBITO-DE. Sexual dysfunction at the onset of type 2 diabetes: the interplay of depression, hormonal and cardiovascular factors. J Sex Med, 11(8), 2065-73.
Corona G, Isidori AM, Aversa A, Bonomi M et al., 2020. Male and female sexual dysfunction in diabetic subjects: Focus on new antihyperglycemic drugs. Rev Endocr Metab Disord, 21(1), 57-65.
Corona G, Monami M, Rastrelli G, Aversa A et al., 2011. Type 2 diabetes mellitus and testosterone: a meta-analysis study. Int J Androl, 34, 528-40.
D'Andrea MR, Lawrence D, Nagele RG, Wang CY et al., 2008. PCNA indexing as a preclinical immunohistochemicalbiomarker for testicular toxicity. Biotech Histochem, 83(5), 211-220.
Dias TR, Alves MG, Silva BM, Oliveira PF, 2014. Sperm glucose transport and metabolism in diabetic individuals. Mol Cell Endocrinol, 396(1-2), 37-45.
El-Khadragy M, Al-Megrin WA, AlSadhan NA, Metwally DM et al., 2020. Impact of Coenzyme Q10 Administration on Lead Acetate-Induced Testicular Damage in Rats. Oxid Med Cell Longev, 4981386.
Ernster L, Dallner G, 1995. Biochemical, physiological and medical aspects of ubiquinone function. Biochim Biophys Acta, 1271(1), 195-204.
Giacco F, Brownlee M, 2010. Oxidative stress and diabetic complications. Circ Res, 107, 1058-1070.
Kanter M, Aktas C, Erboga M, 2012. Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin- induced diabetic rat testis. Food Chem Toxicol, 50(3-4), 719-25.
Kanter M, Aktas C, Erboga M, 2013. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats. Mol Nutr Food Res, 57(9), 1578-85.
Kaplanoglu GT, Bahcelioglu M, Gozil R Helvacioglu F et al., 2013. Effects of green tea and vitamin E in the testicular tissue of streptozotocin-induced diabetic rats. Saudi Med J, 34(7), 734-43.
Khaki A, Fathiazad F, Nouri M, Khaki A et al., 2010. Beneficial effects of quercetin on sperm parameters in streptozotocin- induced diabetic male rats. Phytother Res, 24(9), 1285-91.
Khaneshi F, Nasrolahi O, Azizi S, Nejati V, 2013. Sesame effects on testicular damage in streptozotocin-induced diabetes rats. Avicenna J Phytomed, 3(4), 347-55.
Kianifard D, Sadrkhanlou RA, Hasanzadeh S, 2011. The histological, histomorphometrical and histochemical changes of testicular tissue in the metformin treated and untreated streptozotocin-induced adult diabetic rats. Vet Res Forum 2, 13-24.
Kianifard D and Rezaee F, 2015. Beneficial Effects of Coenzyme Q10 In Reduction of Testicular Tissue Alteration Following Induction Of Diabetes In Adult Rats. Rom J Diabetes Nutr Metab Dis. 22(1), 019-027.
Lass A, Forster MJ, Sohal RS, 1999. Effects of coenzyme Q10 and alpha-tocopherol administration on their tissue levels by coenzyme Q10. Free Radic Biol Med, 26(11-12), 1375- 1382.
Mazen NF, Elnegris HM, 2013. Role of coenzyme Q10 in testicular damage induced by acrylamide in weaned albino rats:a histological and immunohistochemical study. Egypt J Histol, 36 (1), 164-174.
Moloody-Tappe M, Shahrooz R, Razi M, Zarei L et al., 2018. The Effect of CoQ10 on Testicular Tissue in Rats Treating with Busulfan: Sperm Quality and Histological Changes. Iran. J. Vet. Surg, 13(1), 29-38.
Prosek M, Butinar J, Lukanc B, Fir MM et al., 2008. Bioavailability of water-soluble CoQ10 in beagle dogs. J Pharmaceut Biomed, 47, 918-922.
Ramadan LA, Abd-Allah AR, Aly HA, Saad-el-Din AA, 2002. Testicular toxicity effects of magnetic field exposure and prophylactic role of coenzyme Q10 and L-carnitine in mice. Pharmacol Res, 46(4), 363-70.
Salama M, Tsuji M, Tamura M, Kagawa S, 1998. Impact of aging and diabetes mellitus on the expression of the proliferating cell nuclear antigen in rat testicular tissue. Arch Androl, 40(2), 95-107.
Scarano WR, Messias AG, Oliva SU, Klinefelter GR et al., 2006. Sexual behaviour, sperm quantity and quality after shortterm streptozotocin-induced hyperglycaemia in rats. Int J Androl, 29(4), 482-8.
Steger K, Aleithe I, Behre H and Bergmann M, 1998. The proliferation of spermatogonia in normal and pathological human seminiferous epithelium: an immunohistochemical study using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen. Mol Hum Reprod, 4(3), 227-233.
Özcan Ö, 2017. Streptozotosin (stz) ile diyabet oluşturulmuş sıçanlarda kuersetin’in testis dokusuna etkisi. Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi Sağlık Bilimleri Enstitüsü, Eskişehir.
Ullah A, Khan A, Khan I, 2016. Diabetes mellitus and oxidative stres-A concise review. Saudi Pharm J, 24, 547-553.
Wei M, Ong L, Smith MT, Ross FB et al., 2003. The streptozotocin- diabetic rat as a model of the chronic complications of human diabetes. Heart Lung Circ,12(1), 44-50.
Vural H, Sabuncu T, Arslan SO & Aksoy N, 2001. Melatonin inhibits lipid peroxidation and stimulates the antioxidant status of diabetic rats. J Pineal Res, 31(3), 193-198.
Zeng L, Kong X-T, Su J-W, Xia T-L et al., 2001. Evaluation of germ-cell kinetics in infertile patients with proliferating cell nuclear antigen proliferating index. Asian J Androl, 3, 63-66.
Yazar Katkıları Fikir/Kavram: Yasemin Öznurlu, Tuğba Özaydın Tasarım: Yasemin Öznurlu, Tuğba Özaydın Denetleme/Danışmanlık: Emrah Sur Veri Toplama ve/veya İşleme: Yasemin Öznurlu, Tuğba Özaydın
Analiz ve/veya Yorum: Yasemin Öznurlu, Tuğba Özaydın, Emrah Sur
Kaynak Taraması: Yasemin Öznurlu, Tuğba Özaydın
Makalenin Yazımı: Yasemin Öznurlu, Tuğba Özaydın
Eleştirel İnceleme: Emrah Sur
Etik Onay Sunulan çalışma, 20.01.2015 tarihli 2015/22 karar sayılı Selçuk Üniversitesi Deneysel Tıp Uygulama ve Araştırma Merkezi Deney Hayvanları Etik Kurulu’nun onayı ve izniyle yürütüldü.