ÜMİT AKSOY, Çiler AKISÜ, Şebnem ÜSTÜN, HANDE DAĞCI

tttThe effect of human, horse and fetal bovine sera on the growth of Trichomonas vaginalis

Amaç: Farklı serumların in vitro koşullarda Trichomonas vaginalis üremesi üzerine etkisi araştırılması. Gereç ve yöntem: T. vaginalis izolatlaırın insan, at ve fetal bovin serumu (FBS) içeren 3 ayrı modifiye Diamond besiyerine ekildi. Her 3 ortamda parazitin 24., 72., 120., 168. ve 198. saatlerdeki üreme hızı araştırıldı. Bulgular: Klinik izolat 1'in 24., 72. ve 120. saatlerde üreme hızları, FBS ve at serumuna göre anlamlı düzeyde yüksek bulundu (p < 0.05). Sonuç: T. vaginalis kültüründe kullanılan farklı canlılara ait serumların farklı klinik izolatlarda değişken sonuçlar verebileceği kanısına varıldı.

Anahtar Kelimeler:

Kültür ortamı, Trichomonas vaginalis

Trichomonas vaginalis üremesine insan, at ve fetal bovin serumunun etkisi

Objective: It was detected the effect of different sera on the growth of Trichomonas vaginalis. Material and method: Isolates were cultivated in three different modified Diamond media containing human, horse and fetal bovine sera (FBS). The growth rates of the parasite in all three media were investigated after 24, 72, 120,168 and 198 h. Results: The growth rates of the clinical isolate 1 were significantly higher than the FBS and horse serum after 24, 72 and 120 h (p < 0.05). Conclusions: This has led us to believe that sera from different living beings used in the T. vaginalis culture could yield varying results in different clinical isolates.

Keywords:

Culture Media, Trichomonas vaginalis,

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