Lisozim-Kitosan Bazlı Antimikrobiyal Kaplama Uygulamasının Taze Yumurtanın Mikrobiyolojik Kalitesi Üzerine Etkisi

Yumurta, kabuk gibi doğal bir ambalaja sahip olmasına karşın hızla bozulabilen gıdalardandır. Yumurtalarda depolama boyunca mikrobiyal gelişme hızla artmakta ve iç kalite bozulmaktadır. Bu çalışmada, taze yumurtalar farklı konsantrasyonlarda (%0, 10, 20 ve 60 (w/w)) lisozim içeren kitosan kaplama ile kaplanarak yumurtaların mikrobiyal kalitesine etkinliği; Enterobactericeae, koagülaz pozitif Staphylococcus aureus, Listeria monocytogenes, Salmonella spp., toplam mezofilik aerobik bakteri (TMAB) ve küf-maya sayım değerleri oda şartlarında 5 hafta boyunca belirlenmiştir. Depolamanın sonunda, kontrol grubu yumurtalarda Enterobactericeae, Staphylococcus aureus, toplam aerobik mezofilik bakteri sayımı ve küf-maya değerleri sırası ile 2.35, 2.33, 2.61 ve 2.76 log kob/yumurta olarak tespit edilmiştir. Aynı şekilde kitosan-lisozim antimikrobiyal kaplanan yumurtalarda sırası ile 0.91-1.6, 0.89-1.43, 1.07-2.07 ve 2.17-2.46 log kob/yumurta sonuçları tespit edilmiştir. Ayrıca antimikrobiyal madde içeren kaplamalarla kontrol grubu arasında aerobik mezofilik toplam bakteri, Enterobactericeae, Staphylococcus aureus ve küf-maya sayımları bakımından fark istatistiksel olarak önemli bulunmuştur. Çalışma sonucunda yumurtaların hiç birisinde Salmonella spp. ve Listeria monocytogenes varlığına rastlanılmamıştır.

Effect of Lysozyme-Chitosan Based Coatings on Microbial Quality of Fresh Eggs

Hen eggs are perishable, and their quality may change during storage. Eggs are susceptible to microbial degradation, and interior quality may reduce during storage. In this research, fresh eggs were coated with chitosan and chitosan coatings containing lysozyme (0, 10, 20 and 60% (w/w)) and stored at room temperature for 5 weeks. Enterobactericeae, coagulase positive Staphylococcus aureus, Listeria monocytogenes, Salmonella spp., total mesophilic aerobic bacteria (TMAB) and yeast-mold counts were used for the evaluation of microbial quality of fresh eggs coated with different coatings. At the end of 5 weeks, Enterobactericeae, Staphylococcus aureus, total mesophilic aerobic bacteria and yeast-mold counts of the control samples were 2.35, 2.33, 2.61 and 2.76 log cfu/egg respectively. Similarly, the ranges of Enterobactericeae, Staphylococcus aureus, total mesophilic aerobic bacteria and yeast-mold counts for eggs coated with lysozyme-chitosan antimicrobial coatings were 0.91-1.6, 0.89-1.43, 1.07-2.07, and 2.17-2.46 log cfu/egg, respectively. In addition, the differences in the logarithmic counts of total aerobic mesophilic bacteria, Enterobactericeae, Staphylococcus aureus and mold-yeasts between control and treated fresh eggs were statistically significant. Salmonella spp. and Listeria monocytogenes were not detected in any of the eggs.

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