DNA Komet ve Gaz Kromatografisi/Kütle Spektrometrisi Analizleri ile Işınlanmış Hamburger Köftelerin Tespitinde Depolama Süresi ve Sıcaklığın Etkisi

Bu çalışmada ışınlanmış hamburgerler gaz kromatografisi / kütle spektrometresi ile 2-dodesilsiklobütanon (2-DSB) oluşumu açısından ve DNA Komet analizi ile incelenmişlerdir. Hamburgerler 60Co kaynağı kullanılarak 0.4, 0.8 ve 1.2 kGy olmak üzere hedeflenen 3 farklı dozda ışınlanmışlardır. Örnekler ışınlamadan hemen sonra ve ışınlama sonrası +4°C'de 2, 5 ve 8 gün, -12°C'de 2, 5, 8, 15 ve 30 gün depolanarak analiz edilmişlerdir. Parçalanmış DNA anoda doğru yayılma eğilimindedir ve hasar görmüş hücreler ışınlanmış örneklerde kuyruklu yıldız görüntüsü vermektedirler. Işınlanmamış hamburgerlerde ise DNA parçalanması iki farklı sıcaklıkta (+4° ve -12°C) depolama sırasında görülmektedir. Bu çalışmadan elde edilen sonuçlar 1.2 kGy'lik dozda ışınlanmış hamburgerlerde 2-DSB varlığının ışınlanmamış örneklerde oluşmaması nedeniyle, ışınlanmanın bir indikatörü olarak kullanabileceğini göstermektedir. 2-DSB'nin oluşumu ve tespitine 1.2 kGy'lik dozda ışınlanan hamburgerlerde depolama sıcaklığının etkisi önemli bulunmamıştır.

Effects of Storage Time and Temperature on Detection of Irradiated Hamburgers with DNA Comet and Gas Chromatography/Mass Spectrometry Analyses

This study investigated the formation 2-dodecycylobutanone (2-DCB) with gas chromatography/mass spectrometry (GC/MS) and DNA Comet Assay in irradiated hamburgers. Hamburgers were irradiated using a 60Co source at three targeted absorbed doses of 0.4, 0.8, and 1.2 kGy. The samples were analyzed immediately after irradiation and post irradiation on days 2, 5 and 8 during storage at +4°C, and on days 2, 5, 8, 15 and 30 during storage at -12°C. Fragmented DNA stretched towards the anode and the damaged cells appeared as a comet in irradiated samples. DNA fragmentation in non-irradiated hamburger occurred during storage at two different temperature (+4° and -12°C). The results indicated that the presence of 2-DCB may be used as an irradiation indicator in 1.2 kGy irradiated hamburgers since it does not occur in non-irradiated samples. The effect of storage temperatures on formation and diagnosis of 2-DCB of 1.2 kGy irradiated hamburgers was insignificant.

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