Testis torsiyonunda iskemi-reperfüzyon hasarını önlemede okserutin tedavisinin etkinliği

Amaç: Testis torsiyonu, nadir görülen ancak tedavi edilmediği takdirde fertilite açısından geriye dönüşümsüz değişikliklere yol açabilen acil ürolojik bir patolojidir. Testis koruyucu yaklaşımlara rağmen testis torsiyonlu hastaların uzun dönem takiplerinde subfertilite oranları yüksek seyretmektedir. Bu çalışmada mikronize saflaştırılmış flavonoid fraksiyonu olan ve Okserutin içeren Venoruton’un testis torsiyonu ardından gelişen iskemi-reperfüzyon (İR) hasarı üzerine olan etkisini araştırmayı amaçladık. Gereç ve yöntem: Çalışmada incelenecek toplam 24 Sprague-Dawley cinsi erkek rat, üç gruba randomize olarak ayrıldı. Grup 1: Sham grubu, Grup 2: 2 saat torsiyon ardından 4 saat detorsiyon uygulanan (T/D) grubu, Grup 3: Detorsiyondan 30 dakika önce nazogastrikten Okserutin verilen (T/D + Okserutin) grubu. Altı saatin sonunda tüm gruplardaki ratlara sağ orşiektomi yapıldı ve testisleri biyokimyasal ve histopatolojik incelemeler için çıkarıldı. Biyokimyasal incelemeler amacıyla malondialdehit (MDA), katalaz (CAT), superoksit dismutaz (SOD) ve nitrik oksit (NO) düzeyleri ölçüldü. Bulgular: T/D grubunda MDA düzeyi Sham grubuna göre daha yüksek (p

The effect of oxerutin treatment to prevent ischemia-reperfusion injury in testicular torsion

Objective: Testicular torsion is a rare emergency urological pathology, if not treated; it can lead to irreversible changes in terms of fertility. Subfertility rates remain high even though testis preserving management had been applied in patients with testicular torsion. In this study, we aimed to investigate the effect of Venoruton, which was micronized pure flavonoid fraction and containes Oxerutin, on the ischemia-reperfusion injury developed after testicular torsion. Materials and methods: Total of 24 Sprague-Dawley type male rat which will examined in this study were randomized into three groups: Group 1: Sham operation, Group 2: torsion/detorsion (T / D), Group 3: Oxerutin/detorsion (T/D + Oxerutin). After 2 h torsion and 4 h detorsion, in both torsion groups and after 6 h in sham group, right orchiectomy was performed to all rats and their testes were extracted for biochemical and histopathological examinations. For the biochemical analysis malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and nitric oxide (NO) levels were measured. Results: While the level of MDA in the T/D group was higher than that of the sham group (p<0,05), in the T/D + Oxerutin group the MDA level was significantly lower than T/D group (p<0,05). Also, the level of catalase and SOD was lower in the T/D group than that of the sham group, an increase was detected in the T/D + Oxerutin group according to T/D group in the level of catalase and SOD (p<0,05). Conclusion: It was found that venoprotective agent Oxerutin which also has antioxidant properties, prevents free oxygen radicals damage and strengthened antioxidant defense system when applicated before detorsion.

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