Adem ÖZARSLANDAN, Dilek DINÇER, Refik BOZBUGA, Mustafa İMREN, İbrahim Halil ELEKCIOĞLU

Nevşehir İlinde Patates Kist Nematodlarının Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 ve Globodera pallida Stone 1973 (Tylenchida: Heteroderidae) Belirlenmesi

Patates (Solanum tuberosum L.) kist nematodları (Globodera rostochiensis (Wollenweber) ve Globodera pallida (Stone) önemli verim kayıplarına neden olmakta, Türkiye de dahil olmak üzere dünyadaki birçok ülke, Avrupa ve Akdeniz Bitki Koruma Örgütü’nde (EPPO, A2) karantina zararlıları olarak listelenmiştir. Türkiye'de mevcut patates üretim alanı karantina zararlısı olarak bulaşmaya bağlı olarak azalmakta ve yeni alanlar yeni karantina organizmaları tarafından istila tehdidi altında bulunmaktadır. Bu çalışmada, Türkiye, Nevşehir ilinden 2014-2015 yıllarında bahar mevsiminde patates yetiştirme alanlarında örnekler toplanmıştır. Toprak örnekleri Fenwick aletinde yıkanarak bulaşık örneklerdeki kistler elde edilmiş ve kist popülasyonlarının moleküler olarak teşhisleri yapılmıştır. Kist popülasyonlarının moleküler düzeyde türe özgü PITSr3, PITSp4 ve ITS5 primerleri kullanılarak yapılan teşhiste söz konusu primerlerin 435 bp de bant oluşturduğu tespit edilmiştir. Bu çalışma sonucunda Nevşehir ili patates ekiliş alanlarında patates kist nematodu G. rostochiensis saptanmış olup, bu sonuç İç Anadolu Bölgesi için ilk kayıt olma özelliğini taşımaktadır. Ayrıca, sürveylerde aynı tarlaya ait iki örnekte G. rostochiensis’e rastlanılmış, 250 g toprakta ortalama 60 kist yoğunluğunda olduğu tespit edilmiştir.

Anahtar Kelimeler:

Patates, kist nematodu, moleküler teşhis, yoğunluk

A Study on the Presence of Potato Cyst Nematodes: Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 and Globodera pallida Stone 1973 (Tylenchida: Heteroderidae) in Nevşehir Province, Turkey

Potato (Solanum tuberosum L.) cyst nematodes (Globodera rostochiensis (Wollenweber) and Globodera pallida (Stone) cause significant yield losses and have been listed as quarantine pests for many countries in the world including Turkey, and the European and Mediterranean Plant Protection Organization (EPPO, A2). In Turkey, the current potato production area is decreasing due to contamination by quarantine agents, and new fields are under threat of invasion by new quarantine organisms. In this study, samples were collected from Nevşehir province, Turkey, in potato growing fields during the spring growing season in 2014-2015. Soil samples were washed using Fenwick’s can and cysts were extracted and identified using molecular markers. The species-specific molecular markers generated a 435 bp using PITSr3, PITSp4 and ITS5 primers. The potato golden cyst nematode, G. rostochiensis was identified in Nevşehir potato production areas and this is the first report for the Central Anatolia Region. In addition, G. rostochiensis was found in two samples of the same field in the surveys. The average cyst nematode density was determined as 60 cysts in 250 g soil.

Keywords:

Potato, cyst nematode, molecular identification, density,

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