Hatice YILDIRAN, GÜLDEN BAŞYİĞİT KILIÇ, AYNUR GÜL KARAHAN

Probiyotik Mayalar ve Özellikleri

Probiyotikler tüketicinin sağlığı üzerinde olumlu etkileri olan mikroorganizmalardır. Probiyotik uygulamalarının sağlık üzerine etkileri hakkında yapılmış pekçok araştırma bulunmaktadır. En yaygın kullanılan probiyotik bakteriler; bifidobakteriler ve laktobasiller, laktokoklar ve streptokoklar gibi laktik asit bakterileridir. İnsanlar için probiyotik olarak kullanılan mikroorganizmalar arasında ayrıca mayalar, basiller ve enterokoklar da bulunmaktadır. Probiyotik mayalar son yıllarda bilim insanları için ilgi çekici bir alan olmuştur. Yapılan bazı çalışmalar Saccharomyces cinsine ait türlerin antibakteriyel ve probiyotik özelliklere sahip olabileceklerini göstermiştir. Saccharomyces boulardii yıllardır insanları farklı gastrointestinal rahatsızlıklarından korumak veya hastalıklardan iyileştirmek amacıyla probiyotik olarak kullanılan, patojenik olmayan bir mayadır. S. boulardii özellikle ilaç endüstrisinde genellikle liyofilize formda kullanılmaktadır. Yapılan bu derleme çalışmasında probiyotikler, mayaların probiyotik olma özellikleri ve probiyotik mayaların kullanım alanları hakkında bilgiler verilmiş ve bu alanda yapılan araştırmalardan örnekler sunulmuştur.

Probiotic Yeasts and Their Properties

Probiotics are a group of organism those confer health benefit to consumers. There are lots of studies about health benefits of probiotic treatments. The more commonly used probiotic bacteria are bifidobacteria and lactic acid bacteria, such as lactobacilli, lactococci and streptococci. Microorganisms that are probiotic to humans also include yeasts, bacilli and enterococci. Probiotic yeasts have become a field of interest to scientists in recent years. Several previous studies showed that members of Saccharomyces genus can possess anti-bacterial and probiotic properties. Saccharomyces boulardii is non-pathogenic yeast used for many years as a probiotic agent to prevent or treat a variety of human gastrointestinal disorders. S. boulardii is commonly used in lyophilized form especially in the pharmaceutical industry. In this review, information about the probiotics, properties of probiotic yeasts, their usage fields is provided and the results of researches in this area has been presented.

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Anonim. 2011. Türk Gıda Kodeksi Etiketleme Yönetmeliği. 29.12.2011. Resmi Gazete 28157-3.mükerrer.
Arora T, Singh S, Sharma RK. 2013. Probiotics: Interaction with gut microbiome and antiobesity potential. Nutrition. 29: 591-596.
Badel S, Bernardi T, Michaud P. 2011. New perspectives for Lactobacilli exopolysaccharides. Biotechnol Adv. 29: 54-66.
Berg R, Bernasconi P, Fowler D, Gautreaux M. 1993. Inhibition of Candida albicans translocation from the gastrointestinal tract of mice by oral administration of Saccharomyces boulardii. J Infect Dis. 168 (5): 1314-1318.
Binetti, A., Carrasco, M., Reinheimer, J., Sua´rez, V., 2013. Yeasts from autochthonal cheese starters: Technological and functional properties. J Appl Microbiol. 115: 434-444.
Bourlioux P, Koletzko B, Guarner F, Braesco V. 2003. The intestine and its microflora are partners for the protection of the host: report on the Danone Symposium ‘The Intelligent Intestine’. Am J Clin Nutr. 78: 675-683.
Brassart D, Schiffrin EJ. 1997. The use of probiotics to reinforce mucosal defence mechanisms. Trends Food Sci Technol. 8: 321-326.
Buzzini P, Martini A. 2002. Extracellular enzymatic activity profiles in yeast and yeast-like strains isolated from tropical environments. J Appl Microbiol. 93: 1020-1025.
Coman MM, Cecchini C, Verdenell, MC, Silvi S, Orpianes C, Alberto C. 2012. Functional foods as carriers for SYNBIO® , a probiotic bacteria combination. Int J Food Microbiol. 157: 346-352.
Czerucka D, Dahan S, Mograbi B, Rossi B, Rampal P. 2000. Saccharomyces boulardii preservesv the barrier function and modulates the signal transduction pathway induced in enteropathogenic Escherichia coli infected T84 cells. Infect Immun. 68: 5998-6004.
Czerucka D, Piche T, Rampal P. 2007. Review article: yeast as probiotics- Saccharomyces boulardii. Aliment Pharmacol Ther. 26: 767-778.
Czerucka D, Rampal P. 2002. Experimental effects of Saccharomyces boulardii on diarrheal pathogens. Microbes Infect. 4: 733-739.
Çakır İ. 2003. Laktobasillus ve Bifidobakterilerde bazı özelliklerin belirlenmesi. Doktora Tezi. Ankara Üniversitesi Fen Bilimleri Enstitüsü. Ankara, Türkiye. 84 sayfa.
Douradinha B, Reis VC, Rogers MB, Torres FA, Evans JD, Marques ET Jr. 2013. Novel insights in genetic transformation of the probiotic yeast Saccharomyces boulardii. Bioengineered. 5 (1): 21-29.
Edwards-Ingram L, Gitsham P, Burton N, Warhurst G, Clarke I, Hoyle D, Oliver SG, Stateva L. 2007. Genotypic and physiological characterization of Saccharomyces boulardii, the probiotic strain of Saccharomyces cerevisiae. Appl Environ Microbiol. 73 (8): 2458-2467.
FAO. 2002. Food and Nutrition Paper, Probitics in food. Health and Nutritional Properties of Probiotics and Guidelines for Evaluation, World Health Organization, Food and Agriculture Organization of the United Nations, Rome, ftp://ftp.fao.org/es/esn/food/wgreport2.pdf.
Fietto JLR, Araújo RS, Valadão FN, Fietto LG, Brandão RL, Neves MJ, Gomes FCO, Nicoli JR, Castro IM . 2004. Molecular and physiological comparisons between Saccharomyces cerevisiae and Saccharomyces boulardii. Can J Microbiol. 50: 615-621.
Fortina MG, Ricci G, Borgo F, Manachini PL, Arends K, Schiwon K, Abajy MY, Grohmann E. 2008. A survey on biotechnological potential and safety of the novel Enterococcus species of dairy origin, E. İtalicus. Int. J. Food Microbiol.,123: 204-211.
Furukawa S, Watanabe T, Toyama H, Morinaga Y. 2013. Significance of microbial symbiotic coexistence in traditional fermentation. J Biosci Bioeng. 116 (5): 533-539.
Galdeano CM, de LeBlanc AM, Vinderola G, Bonet MEB, Perdigon G. 2007. Proposed model: mechanisms of immunomodulation induced by probiotic bacteria. Clin Vaccine Immunol. 14 (5): 485-492.
Ghada, SI, Manal GM, Mohsen MSA, Eman AG. 2012. Production and biological evaluation of exopolysaccharide from isolated Rhodotorula glutinins. Aust J Basic Appl Sci. 6 (3): 401-408.
Gientka I, Bzducha-Wróbel A, Stasiak-Różańska L, Bednarska AA, Błażejak S. 2016. The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources. Electron J Biotechnol. 22: 31-37.
Hasan MN, Sultan MZ, Mar-E-Um M. 2014. Significance of fermented food in nutrition and food science. J Sci Res. 6 (2): 373-386.
Hassanein SM, Soliman NK. 2010. Effect of probiotic (Saccharomyces cerevisiae) adding to diets on intestinal microflora and performance of Hy-line layers hens. J Am Sci. 6 (11): 159-169.
Hatoum R, Labrie S, Fliss I. 2012. Antimicrobial and probiotic properties of yeasts: from fundamental to novel applications. Frontiers in Microbiology. 3: 1-11.
Hirimuthugoda NY, Chi Z, Wu L. 2007. Probiotic yeasts with phytase activity identified from the gastrointestinal tract of sea cucumbers. SPC Beche de Mer Information Bulletin. 26: 31-34.
Hod K, Ringel Y. 2016. Probiotics in functional bowel disorders. Best Pract Res Clin Gastroenterol. 30: 89-97.
Im E, Pothoulakis C. 2010. Recent advances in Saccharomyces boulardii research. Gastroenterol Clin Biol. 34 (1): 62-70.
Isolauri E, Sütas Y, Kankaanpää P, Arvilommi H, Salminen S. 2001. Probiotics: effects on immunity. Am J Clin Nutr. 73: 444-450.
Klaenhammer TR, Kullen MJ. 1999. Selection and design of probiotics. Int J Food Microbiol. 50: 45-57.
Kobyliak N, Conte1 C, Cammarota G, Haley AP, Styriak I, Gaspar L, Fusek J, Rodrigo L, Kruzliak P. 2016. Probiotics in prevention and treatment of obesity: a critical view. Nutr Metabol. 13: 14.
Ksonzeková P, Bystrickyb P, Vlckováb S, Pätoprst´yb, V, Pulzováa L, Mudroˇnováa D, Kubaskováa T, Csanka T, Tkáciková L. 2016. Exopolysaccharides of Lactobacillus reuteri: Their influence on adherence of E. coli to epithelial cells and inflammatory response. Carbohydr Polym. 141: 10-19.
Kumari S, Panesar PS, Penesar R. 2011. Production of β- Galactosidase using novel yeast isolate from whey. Int J Dairy Sci. 6 (2): 150-157.
Kumura H, Tanoue Y, Tsukahara M, Tanaka T, Shimazaki K. 2004. Screening of dairy yeast strains for probiotic applications. J Dairy Sci. 87: 4050-4056.
Lauranne AAP, Derikx LA, Dieleman FH. 2016. Probiotics and prebiotics in ulcerative colitis. Best Pract Res Clin Gastroenterol. 30: 55-71.
Lichtenstein L, Irit AB, Ofer BB. 2016. Probiotics and prebiotics in Crohn's disease therapies. Best Pract Res Clin Gastroenterol. 30: 81-88.
Lourens-Hattingh A, Viljoen BC. 2001. Growth and survival of a probiotic yeast in dairy products. Food Res Inter. 34: 791- 796.
Mahdhi A, Hmila Z, Behi A, Bakhrouf A. 2011. Preliminary characterization of the probiotic Properties of Candida famata and Geobacillus thermoleovorans. Iran J of Microbiol. 3 (3): 129-134.
McFarland LV. 2010. Systematic review and meta-analysis of Saccharomyces boulardii in adult patients. World J Gastroenterol. 16 (18): 2202-2222.
Moslehi-Jenabian S, Pedersen LL, Jespersen L. 2012. Beneficial effects of probiotic and food borne yeasts on human health. Nutrients. 2: 449-473.
Nagpal R, Kumar A, Kumar M, Behare PV, Jain S, Yadav H. 2012. Probiotics, their health benefits and applications for developing healthier foods: a review. FEMS Microbiol Lett. 334: 1-15.
Nimrat S, Vuthiphandchai V. 2011. In vitro evaluation of commercial probiotic products used for marine shrimp cultivation in Thailand. Afr J Biotechnol. 10 (22): 4643- 4650.
Ogunremi OR, Sanni AI, Agrawal R. 2015. Hypolipidaemic and antioxidant effects of functional cereal-mix produced with probiotic yeast in rats fed high cholesterol diet. J Funct Foods. 17: 742-748.
Palma ML, Zamith-Miranda D, Martins FS, Bozza FA, Nimrichter L, Montero-Lomeli M, Marques ET Jr, Douradinha B. 2015. Probiotic Saccharomyces cerevisiae strains as biotherapeutic tools: is there room for improvement? Appl Microbiol Biotechnol. 99: 6563-6570.
Patel AK, Michaud P, Singhania RR, Soccol CR, Pandey A. 2010. Polysaccharides from probiotics: New developments as food additives. Food Technol Biotechnol. 48 (4): 451- 463.
Pedersen LL, Owusu-Kwarteng J, Thorsen L, Jespersen L. 2012. Biodiversity and probiotic potential of yeasts isolated from Fura, a West African spontaneously fermented cereal. Int J Food Microbiol. 159: 144-151.
Pennacchia C, Blaiotta G, Pepe O, Villani F. 2008. Isolation of Saccharomyces cerevisiae strains from different food matrices and their preliminary selection for a potential use as probiotics. J Appl Microbiol. 105: 1919-1928.
Prajapati P, Patel M, Krishnamurthy R. 2013. Saccharomyces boulardii - A Probiotic of Choice. CIBTech J Biotech. 2 (2): 1-6.
Profir AG, Buruiana CT, Vizireanu C. 2015. Effects of S. cerevisiae var. boulardii in gastrointestinal disorders. J Agroaliment Proc Technol. 21 (2): 148-155.
Rajakala P, Selvi PK. 2006. The effect of pH, temperature and alkali metal ions on the hydrolsis of whey lactose catalysed by β-Galactosidase from Kluyveromyces marxianus. Int J Dairy Sci. 1: 167-172.
Rajkowska A, Kunicka-Styczynska A. 2010. Probiotic properties of yeasts isolated from chicken feces and kefirs. Pol J Microbiol. 59 (4): 257-263.
Ramirez MAJ. 2016. Characterization and safety evaluation of exopolysaccharide produced by Rhodotorula minuta BIOTECH 2178. Int J Food Eng. 2 (1): 31-35.
Rashid M, Sultana M. 2016. Role of probiotics in human and animal health review. J Probiotics Health. 4: 148.
Romanin DE, Llopis S, Genovés S, Martorell P, Ramón VD, Garrote GL, Rumbo M. 2015. Probiotic yeast Kluyveromyces marxianus CIDCA 8154 shows antiinflammatory and anti-oxidative stress properties in in vivo models. Benef Microbes. 7 (1): 83-93.
Roy CC, C Kien L, Bouthillier Lb, Levy E. 2006. Short-Chain Fatty Acids: Ready for Prime Time. Nutr Clin Pract. 21: 351-366.
Saad N, Delattre C, Urdaci M, Schmitter JM, Bressollier P. 2013. An overview of the last advances in probiotic and prebiotic field. Food Sci Technol. 50: 1-16.
Salminen S, Ouwehand AC, Benno Y, Lee YK. 1999. Probiotics: how should they be defined? Trends Food Sci Technol. 10: 107-110.
Salminen S, Ouwehand AC, Isolauri E. 2002. Probiotics: an overview of beneficial effects. Antonie van Leeuwenhoek. 82 (1-4): 279-89.
Sarıkaya H., Aslım B., Yüksekdağ ZN. 2016. Assessment of anti-biofilm activity and bifidogenic growth stimulator (BGS) effect of lyophilized exopolysaccharides (l-EPSs) from lactobacilli strains. Int J Food Prop, 20 (2): 362-371.
Selim ShM, Galal GF, Sharaf MS, Zayed MS. 2013. Evaluation of probiotic bacteria exo-polysaccharides on immune system. Life Sci J. 10 (1): 2719-2725.
Smith IM, Baker A, Arneborg N, Jespersen L. 2015. NonSaccharomyces yeasts protect against epithelial cell barrier disruption induced by Salmonella enterica subsp. enterica serovar Typhimurium. Lett Appl Microbiol. 61: 491-497. Stanton C, Ross RP, Fitzgerald GF, Sinderen DW. 2005. Fermented functional foods based on probiotics and their biogenic metabolites. Curr Opin Biotechnol. 16: 198-203.
Strompfova V, Laukova A, Ouwehand AC. 2004. Selection of enterococci for potential canine probiotic additives. Veterinary Microbiol. 100: 107-114.
Syal P, Vohra A. 2013. Probiotic potential of yeasts isolated from traditional Indian fermented foods. Int J Microbiol Res. 5 (2): 390-398.
Tareb R, Bernardeau M, Gueguen M. Vernoux J-P. 2013. In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni. J Med Microbiol. 62: 637-649.
Upadrasta A, Madempudi RS. 2016. Probiotics and blood pressure: current insights. Integr Blood Press Control. 9: 33- 42.
Vaughan-Martini A. 2003. Reflections on the classification of yeasts for different end-users in biotechnology, ecology, and medicine. Int Microbiol. 6: 175-182.
Verma N, Kumar K, Kaur G, Anand S. 2007. L-asparaginase: a promising chemotherapeutic agent. Crit Rev Biotechnol. 27 (1): 45-62. Vohra A, Syal P, Madan A. 2016. Probiotic yeasts in livestock sector. Anim Feed Sci Technol. 219: 31-47.
Vohra A, Satyanarayana T. 2003. Phytases: Microbial sources, production, purification, and potential biotechnological applications. Crit Rev Biotechnol. 23 (1): 29-60.
Vuyst DL, Degeest B. 1999. Heteropolysaccharides from lactic acid bacteria. FEMS Microbiol Rev. 23 (2): 77-153.
Wang H, Zhang X, Cheng FS, Luo YJ, Dong PC. 2012. Effects of yeast polysaccharide on immune enchancement and production performance of rats. J Anim Vet Adv. 11 (11): 1800-1804.
Yıldıran H, Başyiğit Kılıç G, Karahan AG. 2016. The comparison of the yeast isolates from different sources with the commercial Saccharomyces boulardii preparates for probiotic properties. 1st Black Sea Association of Food Science and Technology Congress. 22-24 Eylül 2016. Ohrid Macedonia. Book of Abstracts, 27-28.
Zhang YJ, Li S, Gan RY, Zhou T, Xu DP, Li HB. 2015. Impacts of gut bacteria on human health and diseases. Int J Mol Sci. 16: 7493-7519.