The present study was conducted to investigate the effectiveness of lycopene in alleviating the toxicity of cypermethrin (CYP) on haematological parameters in carp, Cyprinus carpio. Fish (totally 140 fish) were exposed to sublethal concentrations (0.202 and 0.404 μg/L) of CYP, and lycopene (10 mg per kg of fish weight) was simultaneously administered. At the end of 28 days administration, blood samples were collected and haematological changes (red and white blood cell count, haemoglobin concentration, haematocrit level, and erythrocyte indices: mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration) were determined. According to the data obtained, CYP was determined to lead to negative alterations in the haematological parameters investigated. The administration of lycopene alleviated this effect.
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Atamanalp M, Cengiz M. 2002. The effects of sublethal doses of a synthetic pyrethroid (cypermethrine) on haemoglobin, haematocrit and sediment of Capoeta capoeta capoeta (Güldenstaedt, 1772). EU J Fish Aqua Sci., 19(1-2): 169 – 175
Aydın R, Köprücü K, Dörücü M, Köprücü SŞ, Pala M. 2005. Acute toxicity of synthetic pyrethroid cypermethrin on the common carp (Cyprinus carpio L.) embryos and larvae. Aquacult Int., 13: 451–458. DOI: 10.1007/s10499-005-0615-5.
Boileau WM, Boileau AC, Erdman Jr JW. 2002. Bioavailability of all-trans and cis isomers of lycopene. Exp Biol Med 227: 914– 919.
David M, Shivakumar HB, Shivakumar R, Mushigeri B, Ganti BH. 2003. Toxicity evaluation of cypermethrin and its effect on oxygen consumption of the freshwater fish Tilapia mossambica. Indian J Environ Toxcol., 13: 99–102.
Dotta G Andrade JIA, Gonçalves ELT, Brum A, Mattos JJ, Maraschin M, Martins ML. 2014. Leukocyte phagocytosis and lysozyme activity in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis. Fish Shellfish Immun., 39: 280-284. DOI: 10.1016/j.fsi.2014.05.020.
Drabkin DL. 1946. The crystallographic and optical properties of the hemoglobin of man in comparison with those of other species. J Biol Chem., 164: 703–723.
Kumar N, Prabhu PAJ, Pal AK, Remya S, Aklakur M, Rana RS, Gupta S, Raman RP, Jadhao SB. 2011. Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pestic Biochem Physiol., 99: 45–52. DOI: 10.1016/j.pestbp.2010.10.003.
Li ZH, Velisek J, Grabic R, Li P, Kolarova J, Randak T. 2011. Use of hematological and plasma biochemical parameters to assess the chronic effects of a fungicide propiconazole on a freshwater teleost. Chemosphere 83: 572–578. DOI: 10.1016/j.chemosphere.2010.12.024.
Masud S, Singh IJ. 2013. Effect of cypermethrin on some hematological parameters and prediction of their recovery in a freshwater Teleost, Cyprinus carpio. Afr J Environ Sci Tech., 7(9): 852-856.
Mişe Yonar S. 2013. Toxic effects of malathion in carp, Cyprinus carpio carpio: Protective role of lycopene. Ecotoxicol Environ Saf., 97: 223–229. DOI: 10.1016/j.ecoenv.2013.07.020.
Mişe Yonar S, Ural, MŞ, Silici S, Yonar ME. 2014. Malathioninduced changes in the haematological profile, the immune response, and the oxidative/antioxidant status of Cyprinus carpio carpio: Protective role of propolis. Ecotoxicol Environ Saf., 102: 202–209. DOI: 10.1016/j.ecoenv.2014.01.007
Mişe Yonar S, Yonar ME, Yöntürk Y, Pala A. 2014. Effect of ellagic acid on some haematological, immunological and antioxidant parameters of rainbow trout (Oncorhynchus mykiss). J Anim Physiol An N., 98: 936-941. DOI: 10.1111/jpn.12162.
Modesto, K.A., Martinez, C.B.R., 2010. Effects of Roundup Transorb on fish: Hematology, antioxidant defenses and acetylcholinesterase activity. Chemosphere 81, 781–787. DOI: 10.1016/j.chemosphere.2010.07.005.
Natt MP, Herrick CA. 1952. New blood diluents for counting the erythrocytes and leukocytes of the chicken. Poultry Sci., 31: 735–738.
Prusty AK, Kohli MPS, Sahu NP, Pal AK, Saharan N, Mohapatra S, Gupta SK. 2011. Effect of short term exposure of fenvalerate on biochemical and haematological responses in Labeo rohita (Hamilton) fingerlings. Pestic Biochem Physiol 100: 124–129. DOI: 10.1016/j.pestbp.2011.02.010.
Ramesh M, Saravanan M. 2008. Haematological and biochemical responses in a freshwater fish Cyprinus carpio exposed to chlorpyrifos. Int J Integr Biol., 3: 80–83.
Saglam N, Ispir U, Yonar ME. 2003. The effect of therapeutic bath of malachite green on some haematological parameters of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792). Fresen Environ Bull., 12(10): 1207-1210.
Saglam N, Yonar ME. 2009. Effects of sulfamerazine on selected haematological and immunological parameters in rainbow trout (Onchorhynchus mykiss, Walbaum, 1792). Aquac Res., 40: 395–404. DOI: 10.1111/j.1365-2109.2008.02105.x.
Saravanan M, Kumar KP, Ramesh M. 2011. Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and sublethal exposure to lindane. Pestic Biochem Phys., 100: 206– 211. DOI: 10.1016/j.pestbp.2011.04.002.
Secombes CJ. 1996. The nonspecific immune system: cellular defences. In: Iwama G, Nakanishi T (eds). The fish immune system, organism, pathogen and environment. Academic Press. Toronto. pp 63–103.
Vani T, Saharan N, Mukherjee SC, Ranjan R, Kumar R, Brahmchari RK. 2011. Deltamethrin induced alterations of hematological and biochemical parameters in fingerlings of Catla catla (Ham.) and their amelioration by dietary supplement of vitamin C. Pestic Biochem Physiol., 101: 16–20. DOI: 10.1016/j.pestbp.2011.05.007.
Yonar M.E. 2013. Protective effect of lycopene on oxidative stress and antioxidant status in Cyprinus carpio during cypermethrin exposure. Environ Toxicol., 28(11): 609-616. DOI: 10.1002/tox.20757.
Yonar ME, Mişe Yonar S, Ural MŞ, Silici S, Düşükcan M. 2012. Protective role of propolis in chlorpyrifos-induced changes in the haematological parameters and the oxidative/antioxidative status of Cyprinus carpio carpio. Food Chem Toxicol., 50: 2703–2708. DOI: 10.1016/j.fct.2012.05.032.
Yonar ME, Sakin F. 2011. Ameliorative effect of lycopene on antioxidant status in Cyprinus carpio during pyrethroid deltamethrin exposure. Pestic Biochem Phys., 99: 226–231. DOI: 10.1016/j.pestbp.2010.12.008.