Pınar ÖZIŞIK, Engin YILDIRIM, Mustafa EMİR, Kamer KILINÇ, Erkan YILDIRIM, Kanat ÖZIŞIK

Protective effects of erythropotein and methylprednisolone on lung damage after experimental head injury

Amaç: Sıçanlarda kafa travması sonrası gelişen akciğer hasarında, eritropoetin ve metilprednizolonun akciğer lipid peroksidasyonu ve miyeloperoksidaz seviyesine etkileri araştırıldı. Çalışma planı: Yetmiş altı adet 180-220 gr ağırlığında, dişi Wistar-Albino sıçan 10 gruba ayrıldı. Kafa travması oluşturmak için ağırlık düşürme yöntemi kullanıldı. Örnekler, hasar oluşturulduktan 24 saat sonra sol akciğerden alındı. Akciğer doku miyeloperoksidaz aktivitesi ve lipid peroksidasyon seviyeleri ölçüldü. Lipid peroksidasyon seviyelerindeki ve miyeloperoksidaz aktivitesindeki gruplar arası farklılıkları analiz etmek için tek yönlü varyans analizi (ANOVA) kullanıldı. Daha sonra, post-hoc karşılaştırma yapıldı. Bulgular: Öncelikle, şiddetli travma grubunda lipid peroksidasyon seviyesi ve miyeloperoksidaz aktivitesi önemli ölçüde yüksek bulundu (p

Deneysel kafa travması sonrası gelişen akciğer hasarında, eritropoetin ve metilprednizolonun koruyucu etkisi

Background: The effects of erythropoietin and methylprednisolone on pulmonary lipid peroxidation and myeloperoxidase activity in lung injury following experimental head trauma in rats. Study design: Seventy-six female Wistar-Albino rats, weighing 180-220 gr, were evenly allocated into ten groups. A weight-drop method was used to achieve head trauma. Samples were obtained from the left lung 24-h after the injury. Lung tissue-associated myeloperoxidase activity and lipid peroxidation levels were measured. A one-way analysis of variance (ANOVA) was applied to test the differences in the lipid peroxidation levels and myeloperoxidase activities between groups. Then, post-hoc comparison was performed. Results: Firstly, head trauma substantially elevated lipid peroxidation and myeloperoxidase activity in lung tissue in the severe trauma group (p

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